Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples

Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway

1990 • 721 citations

Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell

1999 • 621 citations

Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle.

1995 • 454 citations

Advances in ultrarapid freezing for the preservation of cellular ultrastructure

1986 • 432 citations

Cellular origin and ultrastructure of membranes induced during poliovirus infection

1996 • 376 citations

Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography

2001 • 352 citations

High‐pressure freezing for the preservation of biological structure: Theory and practice

1989 • 327 citations

High pressure freezing comes of age.

1989 • 324 citations

Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe.

1993 • 320 citations

High-Voltage Electron Tomography of Spindle Pole Bodies and Early Mitotic Spindles in the YeastSaccharomyces cerevisiae

1999 • 283 citations

Freeze substitution of high‐pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water

2002 • 283 citations

High‐pressure freezing of cell suspensions in cellulose capillary tubes

1994 • 237 citations

Nuclear Pore Complex Number and Distribution throughout theSaccharomyces cerevisiaeCell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes

1997 • 217 citations

The potential of cryofixation and freeze substitution: observations and theoretical considerations

1991 • 164 citations

Transmission electron microscopy of yeast

2000 • 162 citations

Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p

2002 • 157 citations

Three-dimensional Organization of Basal Bodies from Wild-Type and δ-Tubulin Deletion Strains ofChlamydomonas reinhardtii

2003 • 153 citations

The influence of high pressure freezing on mammalian nerve tissue

1980 • 149 citations

Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles

1999 • 142 citations

ɛ-Tubulin is required for centriole duplication and microtubule organization

2002 • 125 citations

Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe.

2002 • 113 citations

Freeze Substitution and Low-Temperature Embedding

1992 • 98 citations

Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis

2001 • 95 citations

Cryomethods for thin section electron microscopy

2002 • 91 citations

High-Pressure Freezing Provides New Information on Human Epidermis: Simultaneous Protein Antigen and Lamellar Lipid Structure Preservation. Study on Human Epidermis by Cryoimmobilization

2000 • 65 citations

Basal bodies and centrioles: Their function and structure

1999 • 63 citations

Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze‐substitution

2001 • 60 citations

Advantages of fast‐freeze fixation followed by freeze‐substitution for the preservation of cell integrity

1991 • 57 citations

Electron tomography of yeast cells

2002 • 52 citations

Extragenic Bypass Suppressors of Mutations in the Essential Gene BLD2 Promote Assembly of Basal Bodies With Abnormal Microtubules in Chlamydomonas reinhardtii

2001 • 49 citations

Ultrastructure of Growing Epiphyseal Cartilage Processed by Rapid Freezing and Freeze-Substitution

1983 • 45 citations

Improved specimen support cups and auxiliary devices for the Balzers high pressure freezing apparatus

1987 • 35 citations

Preservation of Cell Ultrastructure by Freeze-Substitution

1991 • 28 citations

Improved method for visualizing coated pits, microfilaments, and microtubules in cryofixed and freeze-substituted plant cells

2002 • 24 citations

Yeast Nuclear Pore Complex Assembly Defects Determined by Nuclear Envelope Reconstruction

2000 • 23 citations

A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution

2001 • 22 citations