Preparation and biological properties of native and recombinant ciliary neurotrophic factor

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Interleukin-1 regulates synthesis of nerve growth factor in non-neuronal cells of rat sciatic nerve

1987 • 1,000 citations

Ciliary neurotrophic factor prevents the degeneration of motor neurons after axotomy

1990 • 840 citations

Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.

1987 • 819 citations

A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence.

1990 • 770 citations

Differential regulation of mRNA encoding nerve growth factor and its receptor in rat sciatic nerve during development, degeneration, and regeneration: role of macrophages.

1987 • 711 citations

Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.

1985 • 710 citations

Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor

1989 • 624 citations

The Receptor for Ciliary Neurotrophic Factor

1991 • 604 citations

Survival effect of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture: comparison with other neurotrophic factors and cytokines

1990 • 591 citations

Nerve growth factor in sympathetic ganglia and corresponding target organs of the rat: correlation with density of sympathetic innervation.

1983 • 564 citations

Neovascularization is associated with a switch to the export of bFGF in the multistep development of fibrosarcoma

1991 • 523 citations

Purification of the Chick Eye Ciliary Neuronotrophic Factor

1984 • 519 citations

Control of Embryonic Motoneuron Survival in Vivo by Ciliary Neurotrophic Factor

1991 • 448 citations

Purification, Cloning, and Expression of Ciliary Neurotrophic Factor (CNTF)

1989 • 429 citations

Growth factors are released by mechanically wounded endothelial cells.

1989 • 420 citations

Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis

1986 • 408 citations

Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture.

1989 • 347 citations

Regional distribution, developmental changes, and cellular localization of CNTF-mRNA and protein in the rat brain.

1991 • 346 citations

Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture

1988 • 343 citations

Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: Inhibition by antibodies to NGF

1991 • 284 citations

Nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section

1985 • 268 citations

Purification of adult rat sciatic nerve ciliary neuronotrophic factor

1986 • 253 citations

Nerve growth activities in rat peripheral nerve

1982 • 235 citations

Two components from eye tissue that differentially stimulate the growth and development of ciliary ganglion neurons in cell culture

1981 • 235 citations

Ciliary neurotrophic factor prevents neuronal degeneration and promotes low affinity NGF receptor expression in the adult rat CNS

1992 • 213 citations

Cell-type-specific regulation of nerve growth factor (NGF) synthesis in non-neuronal cells: comparison of Schwann cells with other cell types

1991 • 211 citations

Prominent expression of acidic fibroblast growth factor in motor and sensory neurons

1991 • 189 citations

Ciliary neurotrophic factor enhances neuronal survival in embryonic rat hippocampal cultures.

1991 • 182 citations

Modes of FGF release in vivo and in vitro

1990 • 169 citations

Histochemical characterization of sensory neurons with high-affinity receptors for nerve growth factor

1989 • 165 citations

Ciliary neurotrophic factor enhances neuronal survival in embryonic rat hippocampal cultures

1991 • 164 citations

Acidic and basic fibroblast growth factors in the nervous system: distribution and differential alteration of levels after injury of central versus peripheral nerve

1991 • 161 citations

Binding characteristics of brain-derived neurotrophic factor to its receptors on neurons from the chick embryo

1988 • 142 citations

Cholinergic Neuronotrophic Factors: Fractionation Properties of an Extract from Selected Chick Embryonic Eye Tissues

1980 • 133 citations

Uptake of nerve growth factor along peripheral and spinal axons of primary sensory neurons

1984 • 125 citations

Non-equivalence of conditioned medium and nerve growth factor for sympathetic, parasympathetic, and sensory neurons

1978 • 123 citations

Influence of nerve growth factor on neurofilament gene expression in mature primary sensory neurons

1990 • 123 citations

Purification and characterization of a trophic factor for embryonic peripheral neurons: Comparison with fibroblast growth factors

1990 • 111 citations

Recombinant Human and Rat Ciliary Neurotrophic Factors

1991 • 96 citations

High ciliary neuronotrophic specific activity in rat peripheral nerve

1984 • 67 citations

Neurite outgrowth elicited by embryonic chick heart: Partial purification of the active factor

1979 • 64 citations

Sequence and structural organization of the human gene encoding ciliary neurotrophic factor

1991 • 63 citations

Isolation and characterization of ciliary neurotrophic factor from rabbit sciatic nerves.

1990 • 52 citations

Purification of a Ciliary Neurotrophic Factor from Bovine Heart

1987 • 50 citations

Decreased synthesis of ciliary neurotrophic factor in degenerating peripheral nerves

1992 • 49 citations

Some aspects of porphyrin neurotoxicity in vitro

1982 • 38 citations

Synthesis, purification, and characterization of human ciliary neuronotrophic factor from E. coli

1991 • 37 citations

Comparative screening for ciliary neurotrophic activity in organs of the rat and chicken

1987 • 14 citations