The Sialyltransferase “Sialylmotif” Participates in Binding the Donor Substrate CMP-NeuAc

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

Glycosyltransferases

1989 • 904 citations

Sialic acids : chemistry, metabolism and function

1982 • 419 citations

Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor.

1987 • 408 citations

Alteration of Terminal Glycosylation Sequences on N-linked Oligosaccharides of Chinese Hamster Ovary Cells by Expression of β-Galactosideα2,6-Sialyltransferase

1989 • 272 citations

Purification of a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase and a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase to homogeneity from rat liver.

1982 • 248 citations

Cloning of a novel alpha 2,3-sialyltransferase that sialylates glycoprotein and glycolipid carbohydrate groups.

1994 • 223 citations

Sialylation of glycoprotein oligosaccharides N-linked to asparagine. Enzymatic characterization of a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase and a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase from rat liver.

1982 • 218 citations

Isolation of GD3 synthase gene by expression cloning of GM3 alpha-2,8-sialyltransferase cDNA using anti-GD2 monoclonal antibody.

1994 • 200 citations

Primary structure of Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase determined by mass spectrometry sequence analysis and molecular cloning. Evidence for a protein motif in the sialyltransferase gene family.

1992 • 191 citations

Expression cloning of a novel Gal beta (1-3/1-4) GlcNAc alpha 2,3-sialyltransferase using lectin resistance selection.

1993 • 187 citations

Polymerase chain reaction cloning of a developmentally regulated member of the sialyltransferase gene family

1993 • 180 citations

Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose.

1977 • 179 citations

Enzymatic properties of beta-D-galactoside alpha2 leads to 6 sialytransferase from bovine colostrum.

1977 • 176 citations

Cloning and expression of the Gal beta 1, 3GalNAc alpha 2,3-sialyltransferase.

1992 • 163 citations

Expression cloning of a GM3-specific alpha-2,8-sialyltransferase (GD3 synthase).

1994 • 145 citations

Complete cDNA sequence encoding human β-galactoside α-2, 6-sialyltransferase

1990 • 140 citations

The signal anchor and stem regions of the beta-galactoside alpha 2,6-sialyltransferase may each act to localize the enzyme to the Golgi apparatus.

1992 • 134 citations

Conversion of a Golgi apparatus sialyltransferase to a secretory protein by replacement of the NH2-terminal signal anchor with a signal peptide

1989 • 126 citations

Expression cloning of a CMP-NeuAc:NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1'Cer alpha 2,8-sialyltransferase (GD3 synthase) from human melanoma cells.

1994 • 116 citations

Cloning and expression of cDNA for a new type of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase

1994 • 113 citations

The HB-6, CDw75, and CD76 differentiation antigens are unique cell-surface carbohydrate determinants generated by the beta-galactoside alpha 2,6-sialyltransferase.

1992 • 112 citations

Molecular cloning and expression of GalNAc alpha 2,6-sialyltransferase.

1994 • 101 citations

Molecular cloning and expression of Galβ1,3GalNAcα2,3‐sialyltransferase from mouse brain

1993 • 98 citations

Transfer of synthetic sialic acid analogs to N- and O-linked glycoprotein glycans using four different mammalian sialyltransferases

1989 • 70 citations

Both potential dolichol recognition sequences of hamster GlcNAc-1-phosphate transferase are necessary for normal enzyme function

1993 • 41 citations

Two step single primer mediated polymerase chain reaction. Application to cloning of putative mouse, β-galactoside α2,6-sialyltransferase cDNA

1993 • 40 citations

Molecular cloning and expression of chick embryo Galβ1,4GlcNAcα2,6‐sialyltransferase

1994 • 39 citations

Textbook of Biochemistry

1952 • 11 citations