Preservation of insulin mRNA levels and insulin secretion in HIT cells by avoidance of chronic exposure to high glucose concentrations.

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1984 • 4,899 citations

Isolation of High‐Molecular‐Weight DNA from Mammalian Cells

1973 • 1,723 citations

Glucose Toxicity

1990 • 742 citations

Relationships Between Fasting Plasma Glucose Levels and Insulin Secretion During Intravenous Glucose Tolerance Tests

1976 • 493 citations

Partial pancreatectomy in the rat and subsequent defect in glucose-induced insulin release.

1983 • 466 citations

Hyperglycaemia as an inducer as well as a consequence of impaired islet cell function and insulin resistance: implications for the management of diabetes

1985 • 461 citations

Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.

1981 • 381 citations

Effect of chronic hyperglycemia on in vivo insulin secretion in partially pancreatectomized rats.

1987 • 335 citations

Increase in insulin response after treatment of overt maturity-onset diabetes is independent of the mode of treatment

1980 • 241 citations

Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase

1985 • 209 citations

Arginine-stimulated acute phase of insulin and glucagon secretion in diabetic subjects.

1976 • 208 citations

A role for alpha-adrenergic receptors in abnormal insulin secretion in diabetes mellitus.

1976 • 178 citations

Regulation of insulin gene expression by glucose and calcium in transfected primary islet cultures.

1990 • 174 citations

Minimal chronic hyperglycemia is a critical determinant of impaired insulin secretion after an incomplete pancreatectomy.

1988 • 172 citations

The Glucose Receptor A DEFECTIVE MECHANISM IN DIABETES MELLITUS DISTINCT FROM THE BETA ADRENERGIC RECEPTOR

1973 • 166 citations

Beta-cell function improved by supplementing basal insulin secretion in mild diabetes.

1976 • 150 citations

The defective glucose sensitivity of the B cell in non insulin dependent diabetes. Improvement after twenty hours of normoglycaemia

1982 • 145 citations

A Role for Prostaglandin E in Defective Insulin Secretion and Carbohydrate Intolerance in Diabetes Mellitus

1977 • 144 citations

Quantitative comparison of mRNA levels in mammalian tissues: 28S ribosomal RNA level as an accurate internal control

1989 • 119 citations

Improvement of Insulin Secretion but Not Insulin Resistance after Short Term Control of Plasma Glucose in Obese Type II Diabetics*

1982 • 114 citations

Severe Diabetes Induced in Subtotally Depancreatized Dogs by Sustained Hyperglycemia

1988 • 113 citations

Coupling of β-Cell Desensitization by Hyperglycemia to Excessive Stimulation and Circulating Insulin in Glucose-Infused Rats

1990 • 101 citations

Insulin secretory responses of a clonal cell line of simian virus 40-transformed B cells

1986 • 97 citations

Improved beta-cell function after intensive insulin treatment in severe non-insulin-dependent diabetes

1988 • 96 citations

A New Phase Of Insulin Secretion: How Will It Contribute to Our Understanding of β-Cell Function?

1989 • 93 citations

Hyperglycemia-induced B cell toxicity. The fate of pancreatic islets transplanted into diabetic mice is dependent on their genetic background.

1990 • 92 citations

Type II Diabetes, Glucose “Non-Sense,” and Islet Desensitization

1989 • 89 citations

Insulin Secretion and cAMP Metabolism in HIT Cells: Reciprocal and Serial Passage-Dependent Relationships

1989 • 78 citations

Receptor-Mediated Adenylate Cyclase—Coupled Mechanism for PGE2 Inhibition of Insulin Secretion in HIT Cells

1987 • 72 citations

Increase in Gs and cyclic AMP generation in HIT cells

1989 • 72 citations

Somatostatin inhibition of Ca2+-induced insulin secretion in permeabilized HIT-T15 cells

1990 • 60 citations

Bombesin stimulates insulin secretion by a pancreatic islet cell line.

1984 • 60 citations

Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40‐transformed B cells

1987 • 54 citations

Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, 3-Isobutyl-1-Methylxanthine, and Potassium

1985 • 49 citations

Type II diabetes, glucose "non-sense," and islet desensitization

1989 • 49 citations

Insulin Response to Glucagon: The Opposing Effects of Diabetes and Obesity

1969 • 43 citations

Pertussis Toxin–Sensitive G Protein Mediation of PGE2 Inhibition of cAMP Metabolism and Phasic Glucose-Induced Insulin Secretion in HIT Cells

1989 • 41 citations

Impaired Insulin Secretion in Human Diabetes Mellitus: The Effect of Naloxone-Induced Opiate Receptor Blockade

1982 • 40 citations

Fuel-Stimulated Insulin Secretion by Clonal Hamster β-Cell Line HIT T-15

1987 • 29 citations

Severe diabetes induced in subtotally depancreatized dogs by sustained hyperglycemia

1988 • 27 citations

Effect of Control of Blood Glucose on Plasma Insulin Responses to Various Stimuli in Secondary Failures to Oral Hypoglycemic Agents and in Newly Diagnosed, Maturity Onset, Ketosis-Resistant Diabetics*

1978 • 24 citations

The Desensitization of Normal B-Cells to Glucosein vitroIs Transient and not Related to High Glucose Levels*

1989 • 23 citations

Insulin secretion and cAMP metabolism in HIT cells. Reciprocal and serial passage-dependent relationships

1989 • 23 citations

Augmented Insulin Responses to Glucose after Secretin Priming in Diabetic Subjects*

1979 • 18 citations

Receptor-mediated adenylate cyclase-coupled mechanism for PGE2 inhibition of insulin secretion in HIT cells

1987 • 14 citations

Insulin release from a cloned hamster B-cell line (HIT-T15) the effects of glucose, amino acids, sulphonylureas and colchicine

1986 • 13 citations

Glycemic control with an artificial pancreas improves insulin responses to both oral and I.V. Glucose in nonobese noninsulin-dependent diabetic subjects

1985 • 8 citations