Approach to stationary two‐dimensional pattern: Influence of focusing time and Immobiline/carrier ampholytes concentrations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid Proteins

1981 • 2,972 citations

Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues

1975 • 1,213 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

Analytical techniques for cell fractions

1980 • 624 citations

Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient

1968 • 413 citations

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

1987 • 271 citations

Analytical techniques for cell fractions

1979 • 177 citations

Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polycrylamide layers

1980 • 174 citations

Improved horizontal two‐dimensional electrophoresis with hybrid isoelectric focusing in immobilized pH gradients in the first dimension and laying‐on transfer to the second dimension

1985 • 124 citations

Membrane protein analysis by isoelectric focusing in immobilized pH gradients

1985 • 118 citations

High resolution two‐dimensional polyacrylamide gel electrophoresis. I. Methodological procedures

1983 • 108 citations

Preparation of immobilized pH gradients spanning 2–6 pH units with two‐chamber mixers: Evaluation of two experimental approaches

1984 • 103 citations

Hybridisoelectric focusing in rehydrated immobilized pH gradients with added carrier ampholytes: Demonstration of human globins

1985 • 92 citations

High-resolution two-dimensional electrophoresis with isoelectric focusing in immobilized pH gradients

1983 • 74 citations

Two-dimensional electrophoresis of membrane and cytosol proteins of mouse liver and brain

1981 • 67 citations

Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergent

1987 • 59 citations

Pore-limit electrophoresis on a gradient of polyacrylamide gel

1968 • 52 citations

Protein precipitation induced by alkaline Immobilines for isoelectric focusing in immobilized pH gradients: Causes and remedies

1987 • 42 citations

Immobilized pH gradients in capillary tubes and two‐dimensional gel electrophoresis

1986 • 41 citations

High resolution two‐dimensional polyacrylamide gel electrophoresis of basic myeloid polypeptides: Use of immobilized pH gradients in the first dimension

1987 • 36 citations

Electrofocusing in Wide pH Range Immobiline Gels Containing Carrier Ampholytes

1985 • 35 citations

Hybrid isoelectric focusing: Adsorption of proteins onto immobilized pH gradient matrices and desorption by carrier ampholytes

1987 • 29 citations

Avoiding liquid exudation on the surface of rehydrated gels used for hybrid isoelectric focusing in carrier ampholyte supplemented immobilized pH gradients

1986 • 27 citations

Two‐dimensional electrophoresis with immobilized pH gradients in the first dimension: Protein focusing as a function of time

1987 • 24 citations

The voltage across wide pH range immobilized pH gradient gels and its modulation through the addition of carrier ampholytes

1986 • 23 citations