pEXPRESS: A family of expression vectors containing a single transcription unit active in prokaryotes, eukaryotes and in vitro

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.

1982 • 1,371 citations

The c-erb-A protein is a high-affinity receptor for thyroid hormone

1986 • 1,340 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Expression cloning of the murine erythropoietin receptor

1989 • 776 citations

Molecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNA

1988 • 677 citations

Retinoic acid and thyroid hormone induce gene expression through a common responsive element

1988 • 586 citations

In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.

1988 • 535 citations

MINIREVIEW: Interactions Among a Subfamily of Nuclear Hormone Receptors: The Regulatory Zipper Model

1990 • 428 citations

‘ATG vectors’ for regulated high-level expression of cloned genes in Escherichia coli

1985 • 392 citations

Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.

1983 • 370 citations

A Domain Containing Leucine-Zipper-Like Motifs Mediate Novelin VivoInteractions between the Thyroid Hormone and Retinoic Acid Receptors

1989 • 293 citations

Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly.

1990 • 245 citations

Mutations of the Rat Growth Hormone Promoter which Increase and Decrease Response to Thyroid Hormone Define a Consensus Thyroid Hormone Response Element

1989 • 240 citations

Direct cloning of leucine zipper proteins: Jun binds cooperatively to the CRE with CRE-BP1

1990 • 221 citations

Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments.

1989 • 210 citations

Maturation of Escherichia coli tryptophan operon mRNA: evidence for 3′ exonucleolytic processing after rho-dependent termination.

1985 • 188 citations

Insertional mutagenesis of the lon gene in Escherichia coli: lon is dispensable

1985 • 168 citations

cis-acting elements of the rat growth hormone gene which mediate basal and regulated expression by thyroid hormone.

1987 • 159 citations

Removal of a terminator structure by RNA processing regulates int gene expression

1984 • 140 citations

c-erbA Protooncogenes Mediate Thyroid Hormone-Dependent and Independent Regulation of the Rat Growth Hormone and Prolactin Genes

1988 • 133 citations

Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis.

1989 • 132 citations

Dimerization among nuclear hormone receptors.

1990 • 71 citations

Hormone and DNA binding activity of a purified human thyroid hormone nuclear receptor expressed in Escherichia coli.

1990 • 56 citations

Characterization of the Domain Structure of Chick c-erbA by Deletion Mutation:in VitroTranslation and Cell Transfection Studies

1989 • 52 citations

Monoclonal antibody to simian virus 40 small t

1984 • 42 citations

Efficient expression of cloned complementary DNAs for secretory proteins after injection into Xenopus oocytes

1984 • 41 citations

Chemical synthesis and molecular cloning of a STOP oligonucleotide encoding an UGA translation terminator in all three reading frames

1983 • 34 citations

Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: Both Fos and Jun contact DNA directly

1990 • 24 citations

Monoclonal antibody analysis of simian virus 40 small t-antigen expression in infected and transformed cells

1989 • 7 citations