High-level expression in Escherichia coli and rapid purification of enzymatically active honey bee venom phospholipase A2

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAP

1991 • 1,709 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response.

1983 • 727 citations

Structure and Properties of a Human Non-pancreatic Phospholipase A2

1989 • 668 citations

Cloning and Recombinant Expression of Phospholipase A2 Present in Rheumatoid Arthritic Synovial Fluid

1989 • 542 citations

Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2

1991 • 447 citations

Crystal Structure of Bee-Venom Phospholipase A ₂ in a Complex with a Transition-State Analogue

1990 • 442 citations

A comparison of the crystal structures of phospholipase A2 from bovine pancreas and Crotalus atrox venom.

1985 • 396 citations

Phospholipase A2 — a mediator between proximal and distal effectors of inflammation

1991 • 384 citations

New Fluorogenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase1

1977 • 383 citations

[33] High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors

1991 • 350 citations

Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins

1984 • 270 citations

Structures of Free and Inhibited Human Secretory Phospholipase A ₂ from Inflammatory Exudate

1991 • 251 citations

X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor

1990 • 241 citations

A sensitive and continuous fluorometric assay for phospholipase A2 using pyrene-labeled phospholipids in the presence of serum albumin

1989 • 241 citations

Structure of recombinant human rheumatoid arthritic synovial fluid phospholipase A2 at 2.2 Å resolution

1991 • 201 citations

Analysis of the cDNA for phospholipase A₂ from honeybee venom glands

1989 • 153 citations

Interfacial catalysis by phospholipase A2: determination of the interfacial kinetic rate constants

1991 • 149 citations

Phospholipase A₂ at the bilayer interface

1991 • 138 citations

Primary structure requirements for the binding of human high molecular weight kininogen to plasma prekallikrein and factor XI.

1987 • 125 citations

The role of Asp‐49 and other conserved amino acids in phospholipases A2 and their importance for enzymatic activity

1989 • 115 citations

The crystal structure of a lysine 49 phospholipase A2 from the venom of the cottonmouth snake at 2.0-A resolution.

1990 • 113 citations

The crystal structure of a lysine 49 phospholipase A2 from the venom of the cottonmouth snake at 2.0-A resolution.

1990 • 99 citations

Construction and Properties of Escherichia coli Strains Exhibiting α-Complementation of β-Galactosidase Fragments In Vivo

1972 • 91 citations

Role of the N-terminus in the interaction of pancreatic phospholipase A2 with aggregated substrates. Properties and crystal structure of transaminated phospholipase A2

1984 • 81 citations

Immunological Methods

1989 • 77 citations

[10] Phospholipase A2-catalyzed hydrolysis of vesicles: Uses of interfacial catalysis in the scooting mode

1991 • 75 citations

Interfacial catalysis by phospholipase A2: monomeric enzyme is fully catalytically active at the bilayer interface

1991 • 72 citations

Interfacial catalysis by phospholipase A2: activation by substrate replenishment

1991 • 72 citations

Phospholipase A2 engineering. X-ray structural and functional evidence for the interaction of lysine-56 with substrates

1991 • 68 citations

Kinetic and inhibition studies of phospholipase A2 with short-chain substrates and inhibitors

1990 • 68 citations

Maximizing gene expression

1986 • 62 citations

Reversible blocking of half-cystine residues of proteins and an irreversible specific deamidation of asparagine-67 of S-sulforibonuclease under mild conditions

1985 • 61 citations

Expression of porcine pancreatic phospholipase A₂. Generation of active enzyme by sequence-specific cleavage of a hybrid protein fromEscherichia coli

1987 • 61 citations

Biochemical characterization of the phospholipase A2 purified from the venom of the Mexican beaded lizard (Heloderma horridum horridum Wiegmann)

1986 • 46 citations

Purification and characterization of five variants of phospholipase A₂ and complete primary structure of the main phospholipase A₂ variant in Heloderma suspectum (Gila Monster) venom

1989 • 37 citations

[14] Bovine and human plasma prekallikrein

1981 • 35 citations

The immunochemistry of sandwich elisas—III. The stoichiometry and efficacy of the protein-avidin-biotin capture (PABC) system

1989 • 31 citations

Purification and characterization of a mutant human platelet phospholipase A₂ expressed in Escherichia coli

1992 • 27 citations

Kinetic characterization of phospholipase A2 modified by manoalogue

1991 • 19 citations

Production of Human Antibodies to Bee Venom Phospholipase A₂ in Vitro

1989 • 15 citations