To disaggregate or not to disaggregate, injury and cell disaggregation, transient or permanent?

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS

1969 • 4,428 citations

Dye Exclusion Tests for Cell Viability

1973 • 903 citations

STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

1953 • 821 citations

AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS

1968 • 707 citations

Production of Plaques in Monolayer Tissue Cultures by Single Particles of an Animal Virus

1952 • 644 citations

Tissue culture, methods and applications

1974 • 634 citations

Cell and tissue culture

1970 • 632 citations

The dissociation and aggregation of cells from organ rudiments of the early chick embryo.

1952 • 411 citations

Cell suspensions from organ rudiments of chick embryos

1952 • 383 citations

Methods of tissue culture

1961 • 340 citations

Experimental Study of the Developing Mammalian Egg: Removal of the Zona Pellucida

1962 • 326 citations

Myosin synthesis in cultures of differentiating chicken embryo skeletal muscle

1972 • 325 citations

CELLS AND TISSUES IN CULTURE

1966 • 312 citations

DEPENDENCE OF SALIVARY EPITHELIAL MORPHOLOGY AND BRANCHING MORPHOGENESIS UPON ACID MUCOPOLYSACCHARIDE-PROTEIN (PROTEOGLYCAN) AT THE EPITHELIAL SURFACE

1972 • 304 citations

Cultivation of Human Breast Carcinomas<xref ref-type="fn" rid="FN1">2</xref>

1958 • 277 citations

The cell surface : its molecular role in morphogenesis

1967 • 271 citations

STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS

1972 • 249 citations

Cell and Tissue Culture

1971 • 246 citations

THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER

1967 • 226 citations

Collagenase: Effect on the Morphogenesis of Embryonic Salivary Epithelium in vitro

1965 • 225 citations

Purification and specificity of pancreatic elastase

1961 • 206 citations

New Complexon for Titration of Calcium in Presence of Magnesium

1957 • 199 citations

Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver

1968 • 199 citations

The Ionic Environment and Metabolic Control

1967 • 189 citations

The Mass Isolation of Whole Cells from Rat Liver

1953 • 169 citations

Interactions of embryonic and fetal neural retina cells with carbohydrate-binding phytoagglutinins: Cell surface changes with differentiation

1972 • 159 citations

Elastase and Elastase-Inhibitor

1949 • 152 citations

Sheets of Pure Epidermal Epithelium from Human Skin

1941 • 152 citations

Effects of collagenase on developing epithelia in vitro: Lung, ureteric bud, and pancreas

1968 • 150 citations

The Isolation of Living Cells from Animal Tissues

1958 • 149 citations

An improved method for the isolation and quantitative cultivation of embryonic cells

1959 • 148 citations

THE EFFECTS OF DIVALENT CATIONS ON TRYPSIN

1953 • 146 citations

THE PARTICULATE ORGANIZATION OF THE CHROMOSOME

1954 • 146 citations

A new method for the preparation of liver cell suspensions

1962 • 141 citations

A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS

1916 • 138 citations

A comparison of methods for estimating the viable count of a suspension of tumour cells

1956 • 138 citations

A Sialomucopeptide Liberated by Trypsin from the Human Erythrocyte

1960 • 137 citations

The collagenase (κ toxin) of Cl. welchii type A

1946 • 135 citations

Inductive interaction of embryonic tissues after dissociation and reaggregation

1958 • 131 citations

[7] Elastase

1970 • 125 citations

Purification and partial characterization of collagenolytic enzymes from Clostridium histolyicum

1968 • 120 citations

Intra- and Extracellular Control of Epithelial Morphogenesis

1971 • 115 citations

The localization of trypsin in cultured mammalian cells

1973 • 115 citations

Viability and growth of mouse embryos after in vitro culture and fusion

1970 • 114 citations

Tissue dissociation with proteolytic enzymes *1Adsorption and activity of enzymes at the cell surface

1971 • 104 citations

Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse

1957 • 103 citations

Production of Albumin and Other Serum Proteins by Clonal Cultures of Normal Human Liver

1971 • 101 citations

A method for the continuous cultivation of mammary epithelium

1971 • 99 citations

The biochemistry of the gas gangrene toxins

1941 • 92 citations

Cultures from adult rat liver cells I. Establishment of monolayer cell‐cultures from normal liver

1971 • 90 citations

Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions.

1956 • 84 citations

Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues

1969 • 83 citations

Cellular adhesiveness in relation to the invasiveness of cancer; electron microscopy of liver perfused with a chelating agent.

1954 • 83 citations

Ionic Permeability and Osmotic Swelling of Cells

1954 • 79 citations

Dissociation of Adult Mouse Liver by Sodium Tetraphenylboron, A Potassium Gomplexing Agent.

1966 • 79 citations

The biochemistry of the gas gangrene toxins

1948 • 75 citations

EFFECT OF ENZYMES AND ACIDITY ON THE ZONA PELLUCIDA OF THE MOUSE EGG BEFORE AND AFTER FERTILIZATION

1964 • 74 citations

CLONAL CULTURE OF DIFFERENTIATED RAT LIVER CELLS

1968 • 73 citations

Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse

1970 • 73 citations

Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse *1II. Observations on the secretory activity

1957 • 72 citations

Methods of Tissue Culture

1962 • 72 citations

Distributions of pancreatic ribonuclease, chymotrypsin, and trypsin in vertebrates

1967 • 60 citations

The role of temperature in the control of aggregation of dissociated embryonic cells

1962 • 57 citations

On the role of metal cations in cellular adhesion: Effect on cell surface charge

1966 • 54 citations

The release of radioactive nucleic acids and mucoproteins by trypsin and ethylenediaminetetra-acetate treatment of baby-hamster cells in tissue culture

1970 • 53 citations

Chemical factors in the mutual adhesiveness of epithelial cells.

1947 • 52 citations

A New Method for Dispersing the Cells of Mammalian Tissues

1964 • 49 citations

The dye-exclusion test for cell viability: Persistence of differential staining following fixation

1972 • 49 citations

EFFECT OF VIRUSES ON EARLY MAMMALIAN DEVELOPMENT, I. ACTION OF MENGO ENCEPHALITIS VIRUS ON MOUSE OVA CULTIVATED IN VITRO

1963 • 48 citations

Intact endoplasmic reticulum and albumin synthesis in rat liver cell suspensions

1971 • 47 citations

SOME EFFECTS OF EDTA AND TETRAPHENYLBORON ON THE ULTRASTRUCTURE OF MITOCHONDRIA IN MOUSE LIVER CELLS

1966 • 47 citations

A Quantitative Method for Growing Animal Cells in vitro

1954 • 47 citations

Surface morphology of trypsinized human cells in vitro

1971 • 45 citations

Mammalian Myocardial Cells11This research was supported in part by USPHS Research Grants NS-09524 from the National Institute of Neurologic Diseases and Stroke and CA-12067 from the National Cancer Institute. It has also benefited from USPHS Training Grant 5-T01-DE-00241–04 from the National Institute of Dental Research. I am pleased to acknowledge the technical assistance of Cindy Arey, Sylvia S. El Kadi, Ann Matthews, James Rodriguez, and Fredy Strasser.

1973 • 44 citations

Preparation and some properties of isolated rat liver cells

1970 • 43 citations

CLEAVAGE OF ONE- AND TWO-CELLED RABBIT EGGS IN VITRO AFTER REMOVAL OF THE ZONA PELLUCIDA

1964 • 42 citations

Incorporation of ¹⁴ C-Labeled Acetate into Lipid by Isolated Foam Cells and by Atherosclerotic Arterial Intima

1967 • 42 citations

Changes in ribosome‐polyribosome balances in chick muscle cells during tissue dissociation, development in culture, and exposure to simplified culture‐medium

1971 • 42 citations

Preparation of Cell Suspensions from Rat Livers

1956 • 41 citations

Reversible injury of L-strain mouse cells by trypsin

1960 • 41 citations

Metabolism of Isolated Liver Cells

1956 • 41 citations

Micromorphologic changes in human amnion cells during trypsinization.

1959 • 40 citations

Mass Separation of Reticuloendothelial and Parenchymal Cells of Rat's Liver

1954 • 40 citations

Chylomicron, free fatty acid and ketone body metabolism of isolated liver cells and liver homogenates

1967 • 39 citations

Some morphological and biochemical characteristics of isolated rat liver cells dissociated with sodium tetraphenylboron and cultured in suspension

1970 • 38 citations

Metabolic Behaviour of Isolated Liver and Kidney Cells

1957 • 38 citations

Dissociation of Chick Embryo Cells by Means of a Chelating Compound

1954 • 38 citations

Axenic mammalian cell reactions

1969 • 37 citations

Collagen solubilization by mammalian proteinases

1960 • 34 citations

Microextensions on Chang's liver cells as observed throughout their division cycle

1969 • 34 citations

Comparison of aggregation of embryonic cells dissociated with trypsin or versene

1967 • 34 citations

The nature of the intercellular material of adult mammalian tissues

1960 • 34 citations

The incorporation of [3H]leucine into protein by tetraphenylboron- and citrate-dispersed rat liver parenchymal cells

1967 • 33 citations

Histotypic self-organization by trypsin-dissociated and EDTA-dissociated chick embryo cells

1968 • 32 citations

Cellular injury resulting from tissue disaggregation

1965 • 31 citations

Phagocytic and Metabolic Activities of Isolated Rat Kupffer Cells

1968 • 30 citations

The use of pronase in tissue culture: A comparison with trypsin

1970 • 27 citations

Further Studies on the Dissociation of Adult Mouse Tissue.

1966 • 26 citations

The adhesion of liver cells

1961 • 26 citations

A CONTROLLED ENVIRONMENT SYSTEM FOR STUDY OF MAMMALIAN CELL PHYSIOLOGY*

1966 • 25 citations

Dissociation of single cells from lung or kidney tissue with elastase

1972 • 24 citations

Biochemical studies on cells isolated from adult rat liver

1970 • 24 citations

A method of obtaining a suspension of intact parenchymal cells from adult rat liver

1971 • 23 citations

Stabilized Suspensions of Monkey Kidney Cells Suitable for Intercontinental Shipment.

1955 • 23 citations

On the single cell state in enzymatically produced tumor cell suspensions

1966 • 23 citations

In vitro stimulation of rat liver cells by homologous partial hepatectomy serum

1971 • 18 citations

Comparison of pronase and trypsin for detachment of human cells during serial cultivation

1966 • 18 citations

Survival of pronase-treated cells in tissue culture

1966 • 18 citations

Propagation of Lactic Dehydrogenase-EIevating Virus in Cell Culture.

1966 • 18 citations

A cautionary note on the use of pronase in tissue culture

1965 • 17 citations

The reaction of the mouse blastocyst and its zona pellucida to enzymes in vitro

1970 • 16 citations

The effect of trypsin or ethylenediaminetetraacetate on the surface of cells in tissue culture

1970 • 16 citations

Human Mammary Tumors

1973 • 15 citations

Two types of long microextensions from cultivated liver cells

1968 • 15 citations

Nonviability of sodium tetraphenylboron-dissociated cells

1969 • 14 citations

Collagenase Treatment of Chick Heart and Thyroid

1973 • 14 citations

Differential effects of metal chelators on Na+, K+-ATPase activity

1973 • 14 citations

Culture of Strain L Cells in Suspension: Replacement of Polymer by Traces of Trypsin in A Defined Medium

1963 • 14 citations

Human Mammary Tumors11The techniques reported in this article were developed with the support of U. S. Public Health Service Grant CA-08515, Contract PH 43-68-1000 from the National Cancer Institute, General Research Support Grant FR-5582 from N. I. H., and Grant-in-Aid Contract M-43 from the State of New Jersey.

1973 • 14 citations

Sequential Enzyme Treatment of Mouse Mammary Gland

1973 • 13 citations

Trypsin sensitivity of mammalian cells grown in a serum-free medium

1971 • 13 citations

THE COLLAGENOLYTIC ACTIVITY OF PANCREAS*

1962 • 13 citations

CHAPTER 3 – Human Mammary Tumors1

1973 • 13 citations

Inhibition of lipid synthesis in isolated rat hepatocytes by serum lipoproteins

1971 • 12 citations

Trypsin

1973 • 12 citations

Effect of temperature on dissociation of adult mouse liver with sodium tetraphenylboron (TPB).

1966 • 12 citations

A New Synthesis of some Derivatives of 4,4′-Stilbenediol

1953 • 11 citations

Metabolism of adult rat liver cells in filter-well culture

1971 • 11 citations

Dissociation and Cultivation of Chick Embryo Cells with an Actinomycete Protease

1963 • 11 citations

A Comparison of Some Biochemical Properties of Dissociated Liver Cells and of Slices

1969 • 11 citations

Pronase

1973 • 11 citations

Effect of Temperature on Dissociation of Adult Mouse Liver with Sodium Tetraphenylboron (TPB).

1966 • 10 citations

Experimental Dissociation of Cells from Chick Embryos

1953 • 10 citations

Cell Dispersal Methods for Increasing Yield from Animal Tissues

1969 • 8 citations

Propagation of mouse hepatitis virus (MHV-3) in monolayer cell cultures from liver of new-born mice

1968 • 7 citations

Inconsistency of the Neutrino Theory of Light

1936 • 7 citations

Some Remarks on the Growth of Human Fibroblasts in Media Containing Copper

1934 • 7 citations

Rhizomorph Formation in Armillaria mellea

1953 • 7 citations

Cell Dispersal Methods for Increasing Yield from Animal Tissues

1969 • 7 citations

The turnover of the fibroblast surface

1968 • 7 citations

The use of pronase for dispersing cells

1967 • 6 citations

Pure primary cultures of adult rat liver macrophages

1971 • 6 citations

The role of oxidative and glycolytic metabolism in cation transport of the isolated rat liver perfused with an erythrocyte-free medium

1971 • 6 citations

A quantitative method of cell culture.

1954 • 6 citations

Surface features of cultured mammalian cells.

1969 • 5 citations

Metabolic and Virologic Studies in Primate Liver Organ Cultures

1970 • 5 citations

Membrane‐bounded intranuclear structures in hepatocytes after exposure to sodium tetraphenyl boron

1971 • 5 citations

Role of'Intercellular'Matrix in Aggregation of Mammalian Cells

1966 • 5 citations

Lysosomal arylsulphatases A and B of rat liver

1970 • 4 citations

Monkey Kidney Cells

1973 • 4 citations

Trypsin

1973 • 4 citations

[Isolation of cells with elastic tissue culture].

1960 • 2 citations