Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

Histological and Histopathological Evaluation of the Testis

1993 • 2,232 citations

Conditions controlling the proliferation of haemopoietic stem cells in vitro

1977 • 2,157 citations

Primary Bioassay of Human Tumor Stem Cells

1977 • 1,625 citations

Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization

1977 • 1,377 citations

Kinetics of spermatogenesis in mammals: seminiferous epithelium cycle and spermatogonial renewal.

1972 • 1,309 citations

The Blood-Testis Barrier in the Rat and the Physiological Compartmentation of the Seminiferous Epithelium1

1970 • 1,119 citations

Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane

1989 • 700 citations

Cell-Cell Interactions in the Testis*

1991 • 660 citations

Characterization of Several Clonal Lines of Cultured Ley dig Tumor Cells: Gonadotropin Receptors and Steroidogenic Responses*

1981 • 596 citations

Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro.

1985 • 538 citations

Gonadotropin binding and stimulation of cyclic adenosine 3':5'-monophosphate and testosterone production in isolated Leydig cells.

1975 • 406 citations

Establishment and Characterization of Two Distinct Mouse Testicular Epithelial Cell Line

1980 • 383 citations

Cooperativity between Sertoli cells and testicular peritubular cells in the production and deposition of extracellular matrix components.

1985 • 331 citations

Immortalization of precursor cells from the mammalian CNS

1988 • 328 citations

Quantitative and Qualitative Characteristics of the Stages and Transitions in the Cycle of the Rat Seminiferous Epithelium: Light Microscopic Observations of Perfusion-Fixed and Plastic-Embedded Testes1

1990 • 302 citations

Immunoreactive sites and accumulation of somatomedin-C in rat Sertoli-spermatogenic cell co-cultures

1986 • 181 citations

Physiological significance of fluid secretion in the testis and blood-testis barrier.

1982 • 167 citations

Rat Testicular Endogenous Steroids and Number of Leydig Cells Between the Fetal Period and Sexual Maturity

1984 • 150 citations

Two alkaline phosphatase genes are expressed during early development in the mouse embryo

1990 • 149 citations

Steroidogenesis in Rat Leydig Cells: Changes in Activity of 5-ane and 5-ene 3β-Hydroxysteroid Dehydrogenases During Sexual Maturation¹

1976 • 145 citations

Isolation of mouse primordial germ cells

1982 • 109 citations

Separation of mouse spermatogenic cells by velocity sedimentation

1972 • 109 citations

Pachytene Spermatocyte Proteins Influence Sertoli Cell Function1

1988 • 105 citations

Cytochrome c: Immunofluorescent Localization of the Testis-Specific Form

1977 • 97 citations

Immortalization by c-myc, H-ras, and Ela oncogenes induces differential cellular gene expression and growth factor responses.

1987 • 82 citations

Identification of a non-mitogenic paracrine factor involved in mesenchymal-epithelial cell interactions between testicular peritubular cells and Sertoli cells

1986 • 81 citations

Simian virus 40-transformed adherent cells from human long-term marrow cultures: cloned cell lines produce cells with stromal and hematopoietic characteristics

1987 • 80 citations

Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse

1987 • 79 citations

Adhesion, Shape, Proliferation, and Gene Expression of Mouse Leydig Cells are Influenced by Extracellular Matrix in Vitro1

1991 • 74 citations

Transcription switch of two phosphoglycerate kinase genes during spermatogenesis as determined with mouse testis sections in situ

1990 • 72 citations

Fine structure of the seminiferous tubule of the Swiss mouse. I. The limiting membrane, sertoli cell, spermatogonia, and spermatocytes

1964 • 65 citations

Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse

1990 • 64 citations

Functional Maturation of Rat Testis Leydig Cells

1984 • 63 citations

Hormonal activation of protein kinase in isolated Leydig cells. Electrophoretic analysis of cyclic AMP receptors.

1978 • 63 citations

Cytochemical and Biochemical Characterization of Testicular Peritubular Myoid Cells1

1989 • 60 citations

Sertoli Cells in Culture: Views from the Perspectives of an in Vivoist and an in Vitroist

1989 • 60 citations

Developmental stages of fetal-type Leydig cells in prepubertal rats

1989 • 59 citations

Paracrine control of immature Sertoli cells by adult germ cells, in the rat (an in vitro study)

1988 • 56 citations

Biology of Mammalian Germ Cell Mutagenesis

1990 • 56 citations

Extracellular Matrix in Testicular Differentiation ^a

1984 • 55 citations

Molecular evolution of the human Pgk-2 retroposon

1990 • 55 citations

Transformation of human cultured fibroblasts with plasmids carrying dominant selection markers and immortalizing potential

1986 • 50 citations

SV40 immortalizes myogenic cells: DNA synthesis and mitosis in differentiating myotubes

1990 • 49 citations

The ultrastructural relationships between Sertoli cells and spermatogenic cells in the rat.

1976 • 48 citations

Vectorial secretion of transferrin and androgen binding protein in Sertoli cell cultures: effect of extracellular matrix, peritubular myoid cells and medium composition

1987 • 45 citations

Immortalization of primary cells by DNA tumor viruses

1990 • 43 citations

Effect of Substrate on the Shape of Sertoli Cells in Vitro^a

1984 • 41 citations

Establishment of two rabbit mammary epithelial cell lines with distinct oncogenic potential and differentiated phenotype after microinjection of transforming genes.

1986 • 40 citations

Basement membrane and epithelial features of fetal-type Leydig cells in rat and human testis

1989 • 36 citations

Effect of Germ Cells on Vectorial Secretion of Androgen Binding Protein and Transferrin by Immature Rat Sertoli Cells In Vitro

1988 • 33 citations

NEONATAL GONOCYTES CO-CULTURED WITH SERTOLI CELLS ON A LAMININ-CONTAINING MATRIX RESUME MITOSIS AND ELONGATE

1991 • 33 citations

Protein Secretory Patterns of Rat Sertoli and Peritubular Cells are Influenced by Culture Conditions1

1986 • 32 citations

Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture

1984 • 31 citations

Actions of Extracellular Matrix on Sertoli Cell Morphology and Function1

1989 • 30 citations

Stromal cells in long-term cultures of liver, spleen, and bone marrow at different developmental ages have different capacities to maintain GM-CFC proliferation.

1991 • 29 citations

Influence of germ cells on Sertoli cell secretory activity in direct and indirect co-culture with Sertoli cells from rats of different ages

1989 • 29 citations

Analyses of Stage-Specific Multiple Forms of Lactate Dehydrogenase and of Cytochrome c during Spermatogenesis in the Mouse

1977 • 25 citations

Cellular immortalization--an essential step or merely a risk factor in DNA virus-induced transformation?

1990 • 18 citations

Somatic and germ cell interactions during histogenetic aggregation of mouse fetal testes

1992 • 18 citations

Characterization of a New Superfusion, Two‐Compartment Culture System for Sertoli Cells: Influence of Extracellular Matrix on the Cell Permeability and Dynamics of Transferrin Secretion

1990 • 13 citations

Selected Enzyme Histochemistry of Sertoli Cells 2. Adult Rat Sertoli Cells in Co-culture with Peritubular Fibroblasts

2009 • 11 citations

Hormonal Communicating Events in the Testis

1989 • 9 citations

An active site mutant of human placental alkaline phosphatase with deficient enzymatic activity and preserved immunoreactivity

1990 • 7 citations

Isolation and Characterization of Rat Fetal Sertoli Cells*

1988 • 5 citations

Selected Enzyme Histochemistry of Sertoli Cells 1. Immature Rat Sertoli Cells in Vitro

2009 • 5 citations