Expression and One-Step Purification of a Fully Active Polyhistidine-Tagged Cytochromebc1Complex fromRhodobacter sphaeroides

A simplification of the protein assay method of Lowry et al. which is more generally applicable

1977 • 8,485 citations

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

Photosynthesis: from Light to Biosphere

1995 • 1,645 citations

Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria

1988 • 1,523 citations

Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc ₁ Complex

1998 • 1,214 citations

Electron transfer by domain movement in cytochrome bc1

1998 • 1,051 citations

Crystal Structure of the Cytochrome bc ₁ Complex from Bovine Heart Mitochondria

1997 • 934 citations

Isolation of ubiquinol oxidase from Paracoccus denitrificans and resolution into cytochrome bc1 and cytochrome c-aa3 complexes.

1985 • 278 citations

Molecular proportion of the fixed cytochrome components of the respiratory chain of Keilin-Hartree particles and beef heart mitochondria

1966 • 181 citations

Cytochrome bc1 complex [2Fe-2S] cluster and its interaction with ubiquinone and ubihydroquinone at the Qo site: a double-occupancy Qo site model

1992 • 171 citations

A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex

1996 • 168 citations

Purification of highly active cytochrome bc1 complexes from phylogenetically diverse species by a single chromatographic procedure

1987 • 166 citations

How rapid are the internal reactions of the ubiquinol:cytochrome c 2 oxidoreductase?

1989 • 158 citations

Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni²⁺‐NTA affinity chromatography

1995 • 155 citations

[56] Determination of contents and redox states of ubiquinone and menaquinone

1978 • 128 citations

Rapid isolation of bacterial photosynthetic reaction centers with an engineered poly-histidine tag

1996 • 118 citations

One-step purification of histidine-tagged cytochrome bo3 from Escherichia coli and demonstration that associated quinone is not required for the structural integrity of the oxidase

1997 • 87 citations

Cloning and DNA sequencing of the fbc operon encoding the cytochrome bc₁ complex from Rhodobacter sphaeroides

1990 • 86 citations

Ubiquinol-cytochrome c oxidoreductase of higher plants. Isolation and characterization of the bc1 complex from potato tuber mitochondria

1991 • 84 citations

Large-scale purification and characterization of a highly active four-subunit cytochrome bc1 complex from Rhodobacter sphaeroides

1990 • 82 citations

Reaction of cytochrome c with imidazole

1969 • 70 citations

Resolved difference spectra of redox centers involved in photosynthetic electron flow in Rhodopseudomonas capsulata and rhodopseudomonas sphaeroides

1981 • 65 citations

Rapid and discrete isolation of oxygen‐evolving His‐tagged photosystem II core complex from Chlamydomonas reinhardtii by Ni²⁺ affinity column chromatography

1998 • 53 citations

Identification of mitochondrial proteins in membrane preparations from Chlamydomonas reinhardtii.

1992 • 48 citations

Thermodynamic and spectroscopic characteristics of the cytochrome bc1 complex

1989 • 44 citations

How Replacements of the 12 Conserved Histidines of Subunit I Affect Assembly, Cofactor Binding, and Enzymatic Activity of the Bradyrhizobium japonicum cbb 3-type Oxidase

1998 • 43 citations

Essentiality of the molecular weight 15,000 protein (subunit IV) in the cytochrome b-c1 complex of Rhodobacter sphaeroides

1991 • 40 citations

Characterization of cytochrome b in the isolated ubiquinol‐cytochrome c₂ oxidoreductase from Rhodopseudomonas sphaeroides GA

1983 • 39 citations

Purification of a Histidine-Tagged Plant Plasma Membrane H+-ATPase Expressed in Yeast

1998 • 35 citations

Ubiquinol:Cytochrome c Oxidoreductase

1997 • 26 citations

Effects of axial ligand replacement on the redox potential of cytochrome c

1995 • 18 citations

Structure and Function in the bc1-Complex of Rhodobacter Sphaeroides

1995 • 9 citations

Reconstitution of F₁‐ATPase activity from Escherichia coli subunits α, β and subunit γ tagged with six histidine residues at the C‐terminus

1998 • 6 citations