Mapping Staphylococcal Nuclease Conformation Using an EDTA-Fe Derivative Attached to Genetically Engineered Cysteine Residues

Tissue sulfhydryl groups

1959 • 25,130 citations

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

The interpretation of protein structures: Estimation of static accessibility

1971 • 5,771 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Protein Oxidation and Aging

1992 • 2,687 citations

[37] Carbonyl assays for determination of oxidatively modified proteins

1994 • 2,605 citations

Formation of a Molten Globule Intermediate Early in the Kinetic Folding Pathway of Apomyoglobin

1993 • 735 citations

Reaction mechanisms in the radiolysis of peptides, polypeptides, and proteins

1987 • 712 citations

Mechanism of acid-induced folding of proteins

1990 • 560 citations

Free radicals, lipids and protein degradation

1986 • 556 citations

[3] The preparation of guanidine hydrochloride

1972 • 540 citations

Cleavage of DNA with methidiumpropyl-EDTA-iron(II): reaction conditions and product analyses

1984 • 477 citations

PATHWAYS OF PROTEIN FOLDING

1993 • 435 citations

[47] A centrifuged-column procedure for the measurement of ligand binding by beef heart F1

1979 • 365 citations

Catalytic Properties and Specificity of the Extracellular Nuclease of Staphylococcus aureus

1967 • 362 citations

Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron.

1985 • 268 citations

The crystal structure of staphylococcal nuclease refined at 1.7 Å resolution

1991 • 260 citations

Residual structure in large fragments of staphylococcal nuclease: effects of amino acid substitutions

1989 • 234 citations

Construction of new ligand binding sites in proteins of known structure

1991 • 233 citations

Three-state analysis of sperm whale apomyoglobin folding

1993 • 226 citations

Transfer of oxygen from an artificial protease to peptide carbon during proteolysis.

1991 • 167 citations

Truncated staphylococcal nuclease is compact but disordered.

1992 • 162 citations

Conformation-dependent cleavage of staphylococcal nuclease with a disulfide-linked iron chelate.

1992 • 144 citations

New strategy for selective protein cleavage

1990 • 134 citations

A magnetization-transfer nuclear magnetic resonance study of the folding of staphylococcal nuclease

1989 • 133 citations

The Amino Acid Sequence of an Extracellular Nuclease of Staphylococcus aureus

1966 • 105 citations

Staphylococcal nuclease folding intermediate characterized by hydrogen exchange and NMR spectroscopy.

1994 • 94 citations

Transfer of a β-turn structure to a new protein context

1989 • 92 citations

Oxidative polypeptide cleavage mediated by EDTA-iron covalently linked to cysteine residues

1993 • 77 citations

Nonenzymatic peptide alpha-amidation. Implications for a novel enzyme mechanism.

1985 • 66 citations

Identification of metal-isocitrate binding site of pig heart NADP-specific isocitrate dehydrogenase by affinity cleavage of the enzyme by Fe(2+)-isocitrate.

1993 • 51 citations

Hydrogen-1 NMR evidence for three interconverting forms of staphylococcal nuclease: effects of mutations and solution conditions on their distribution

1989 • 48 citations

Partially structured self-associating states of acidic fibroblast growth factor

1993 • 47 citations

Fluorescence and conformational stability studies of Staphylococcus nuclease and its mutants, including the less stable nuclease-concanavalin A hybrids

1991 • 44 citations

Reaction and Free Radical Kinetics of Prostaglandin H Synthase with Manganese Protoporphyrin IX as the Prosthetic Group

1994 • 38 citations

Oxidation of the active site of glutamine synthetase: Conversion of arginine-344 to γ-glutamyl semialdehyde

1991 • 37 citations

Effect of proline mutations on the stability and kinetics of folding of staphylococcal nuclease

1993 • 34 citations

pH-Induced folding/unfolding of staphylococcal nuclease: determination of kinetic parameters by the sequential-jump method

1992 • 29 citations

Stress and strain in staphylococcal nuclease

1993 • 27 citations

The sequence location of the actin metal

1992 • 3 citations