Utilization of a Recombinant Substrate rAgg1 to Study the Biochemical Properties of Aggrecanase in Cell Culture Systems

High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment

1984 • 898 citations

PCR Technology: Principles and Applications for DNA Amplification

1990 • 512 citations

Complete coding sequence and deduced primary structure of the human cartilage large aggregating proteoglycan, aggrecan. Human-specific repeats, and additional alternatively spliced forms.

1991 • 446 citations

The structure of aggrecan fragments in human synovial fluid. evidence that aggrecanase mediates cartilage degradation in inflammatory joint disease, joint injury, and osteoarthritis

1993 • 433 citations

The structure of aggrecan fragments in human synovial fluid. Evidence for the involvement in osteoarthritis of a novel proteinase which cleaves the Glu 373-Ala 374 bond of the interglobular domain.

1992 • 412 citations

Differences between sub-populations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix production

1988 • 366 citations

The interglobular domain of cartilage aggrecan is cleaved by PUMP, gelatinases, and cathepsin B.

1992 • 297 citations

Catabolism of aggrecan in cartilage explants. Identification of a major cleavage site within the interglobular domain

1991 • 285 citations

Identification of a stromelysin cleavage site within the interglobular domain of human aggrecan. Evidence for proteolysis at this site in vivo in human articular cartilage.

1992 • 273 citations

Monoclonal antibodies that specifically recognize neoepitope sequences generated by ‘aggrecanase’ and matrix metalloproteinase cleavage of aggrecan: application to catabolism in situ and in vitro

1995 • 212 citations

Aggrecan is degraded by matrix metalloproteinases in human arthritis. Evidence that matrix metalloproteinase and aggrecanase activities can be independent.

1996 • 203 citations

Cell-mediated Catabolism of Aggrecan

1995 • 197 citations

Mechanism of catabolism of aggrecan by articular cartilage

1992 • 182 citations

A calcium-dependent antibody for identification and purification of recombinant proteins.

1989 • 181 citations

Cleavage of cartilage proteoglycan between G1 and G2 domains by stromelysins

1991 • 164 citations

Chondrocyte-mediated depletion of articular cartilage proteoglycans in vitro

1985 • 150 citations

N-terminal sequence of proteoglycan fragments isolated from medium of interleukin-1-treated articular-cartilage cultures. Putative site(s) of enzymic cleavage

1992 • 131 citations

Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373–A374 in the interglobular domain of cartilage aggrecan

1994 • 118 citations

Extracellular matrix metabolism by chondrocytes III. Modulation of proteoglycan synthesis by extracellular levels of proteoglycan in cartilage cells in culture

1977 • 116 citations

Effect of Interleukin-1 and Insulin Like Growth Factor-1 on the Release of Proteoglycan Components and Hyaluronan from Pig Articular Cartilage in Explant Culture

1991 • 102 citations

Degradation of proteoglycan in articular cartilage

1978 • 92 citations

Monoclonal antibodies recognizing protease-generated neoepitopes from cartilage proteoglycan degradation. Application to studies of human link protein cleavage by stromelysin.

1992 • 75 citations

Structure of the human aggrecan gene: exon-intron organization and association with the protein domains

1995 • 71 citations

Extraction and isolation of mRNA from adult articular cartilage

1992 • 57 citations

Quantification of Vertical-Fiber Defect in Cattle Hide by Small-Angle Light Scattering

1991 • 39 citations

Extracellular matrix metabolism by chondrocytes

1978 • 34 citations

The interaction between retinoic acid and the transforming growth factors-β in calf articular cartilage organ cultures

1992 • 26 citations

Cited By (0)

Loading...