Reconstitution of Escherichia coli 50S ribosomal subunits containing puromycin-modified L23: functional consequences

Structure, Function, and Genetics of Ribosomes

1986 • 852 citations

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites

1989 • 492 citations

Total reconstitution and assembly of 50 S subunits from Escherichia coli ribosomes in vitro

1976 • 158 citations

Determination of the location of proteins L14, L17, L18, L19, L22 and L23 on the surface of the 50S ribosomal subunit of Escherichia coli by immune electron microscopy

1974 • 144 citations

Minimal set of ribosomal components for reconstitution of the peptidyltransferase activity.

1982 • 141 citations

Ribosomal components from Escherichia coli 50 S subunits involved in the reconstitution of peptidyltransferase activity.

1981 • 133 citations

Photoincorporation of tetracycline into Escherichia coli ribosomes. Identification of the major proteins photolabeled by native tetracycline and tetracycline photoproducts and implications for the inhibitory action of tetracycline on protein synthesis

1983 • 130 citations

Immunoelectron Microscopy of Ribosomes

1984 • 122 citations

Photo-affinity labelling at the peptidyl transferase centre reveals two different positions for the A- and P-sites in domain V of 23S rRNA.

1988 • 118 citations

Electron microscopy and computer image averaging of ice-embedded large ribosomal subunits from Escherichia coli

1988 • 106 citations

[37] Total reconstitution of 50 S subunits from Escherichia coli ribosomes

1979 • 79 citations

Immunoelectron microscopic localization of puromycin binding on the large subunit of the Escherichia coli ribosome.

1982 • 69 citations

Protein estimation by the product of integrated peak area and flow rate

1989 • 68 citations

Proteins from Escherichia coli ribosomes involved in the binding of erythromycin

1978 • 58 citations

Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli.

1983 • 58 citations

[50] Ribosomal peptidyltransferase: The fragment reaction

1971 • 56 citations

Reverse phase high performance liquid chromatography of Escherichia coli ribosomal proteins: standardization of 70 S, 50 S, and 30 S protein chromatograms.

1983 • 51 citations

Protein components of the erythromycin binding site in bacterial ribosomes.

1988 • 50 citations

Structure of a protein L23-RNA complex located at the A-site domain of the ribosomal peptidyl transferase centre

1984 • 49 citations

Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site

1978 • 48 citations

Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analog of puromycin. II. On the identification of the major covalently labeled ribosomal proteins and on the mechanism of photoincorporation

1982 • 37 citations

[3H]-p-Azidopuromycin photoaffinity labeling of Escherichia coli ribosomes: evidence for site-specific interaction at U-2504 and G-2502 in domain V of 23S ribosomal RNA

1988 • 37 citations

Antibiotic effects on the photoinduced affinity labeling of Escherichia coli ribosomes by puromycin

1979 • 36 citations

Crystallography of ribosomal particles

1988 • 25 citations

Localization of sites of photoaffinity labeling of the large subunit of Escherichia coli ribosomes by arylazide derivative of puromycin.

1985 • 25 citations

Application of high-performance liquid chromatography to the reconstitution of ribosomal subunits

1984 • 24 citations

Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analog of puromycin. III. Evidence for the functional site specificity of labeling

1982 • 23 citations

Reconstitution of Escherichia coli ribosomes containing puromycin-modified S14: functional effects of the photoaffinity labeling of a protein essential for tRNA binding

1986 • 22 citations

Photoaffinity labelling of Escherichia coli ribosomes

1987 • 22 citations

On the structural specificity of puromycin binding to Escherichia coli ribosomes

1985 • 21 citations

[23] Affinity labeling of ribosomes

1988 • 18 citations

Immunoelectron microscopic localisation of ribosomal proteins from Bacillus stearothermophilus that are homologous to Escherichia coli L1, L6, L23 and L29

1988 • 16 citations