Denaturation behavior of antithrombin in guanidinium chloride. Irreversibility of unfolding caused by aggregation

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Simplex Method for Function Minimization

1965 • 27,535 citations

The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

1969 • 20,498 citations

Computed circular dichroism spectra for the evaluation of protein conformation

1969 • 3,654 citations

Specific Intermediates in the Folding Reactions of Small Proteins and the Mechanism of Protein Folding

1982 • 1,041 citations

The Estimation of Polypeptide Chain Molecular Weights by Gel Filtration in 6 M Guanidine Hydrochloride

1969 • 603 citations

Viscosity and Density of Aqueous Solutions of Urea and Guanidine Hydrochloride

1966 • 556 citations

[3] The preparation of guanidine hydrochloride

1972 • 540 citations

Sequence of chicken ovalbumin mRNA

1978 • 438 citations

Purification of antithrombin III by affinity chromatography

1974 • 426 citations

Anticoagulant activity of heparin: Separation of high‐activity and low‐activity heparin species by affinity chromatography on immobilized antithrombin

1976 • 420 citations

A surprising new protein superfamily containing ovalbumin, antithrombin-III, and alpha1-proteinase inhibitor

1980 • 410 citations

The Size and Shape of Human and Bovine Antithrombin III

1977 • 264 citations

Long-column meniscus depletion sedimentation equilibrium technique for the analytical ultracentrifuge

1970 • 235 citations

Folding of Protein Fragments

1981 • 215 citations

Fractionation of low molecular weight heparin species and their interaction with antithrombin.

1979 • 208 citations

Sequence homology between human .alpha.1-antichymotrypsin, .alpha.1-antitrypsin, and antithrombin III

1983 • 204 citations

Angiotensinogen Is Related to the Antitrypsin-Antithrombin-Ovalbumin Family

1983 • 194 citations

Cloning and sequence of cDNA coding for alpha 1-antitrypsin.

1981 • 176 citations

Kinetic aspects of conformational changes in proteins. I. Rate of regain of enzyme activity from denatured proteins

1971 • 156 citations

Binding of low-affinity and high-affinity heparin to antithrombin. Ultraviolet difference spectroscopy and circular dichroism studies

1978 • 140 citations

The Binding of Low-Affinity and High-Affinity Heparin to Antithrombin. Fluorescence Studies

1978 • 136 citations

The active site of antithrombin. Release of the same proteolytically cleaved form of the inhibitor from complexes with factor IXa, factor Xa, and thrombin.

1982 • 134 citations

Purine nucleoside phosphorylase cleaves the C–O bond of ribose 1-phosphate. Evidence from the 18O shift in 31P NMR

1979 • 118 citations

Binding of heparin to human antithrombin III as studied by measurements of tryptophan fluorescence

1977 • 114 citations

A computer-assisted model for estimating protein secondary structure from circular dichroic spectra: Comparison of animal lactate dehydrogenases

1980 • 109 citations

Kinetic aspects of conformational changes in proteins. II. Structural changes in renaturation of denatured proteins

1971 • 108 citations

Acceleration of the Reaction between, Thrombin and Antithrombin III by Non‐stoichiometric Amounts of Heparin

1976 • 93 citations

Release of a Two‐Chain Form of Antithrombin from the Antithrombin‐Thrombin Complex

1979 • 81 citations

Polyethylene glycol 6,000 enhancement of the clotting of fibrinogen solutions in visual and mechanical assays

1974 • 81 citations

Predictions of the secondary structure of antithrombin III and the location of the heparin-binding site.

1984 • 79 citations

Production in vitro and properties of a modified form of bovine antithrombin, cleaved at the active site by thrombin.

1982 • 78 citations

Thermal denaturation of antithrombin III. Stabilization by heparin and lyotropic anions.

1981 • 75 citations

Decreased binding of heparin to antithrombin following the interaction between antithrombin and thrombin

1977 • 73 citations

The covalent nature of the human antithrombin III–thrombin bond

1980 • 72 citations

Dissociation of complexes and their derivatives formed during inhibition of bovine thrombin and activated factor X by antithrombin III.

1979 • 71 citations

The kinetics of formation and dissociation of the bovine thrombin.antithrombin III complex.

1979 • 68 citations

Antibody as an immunological probe for studying the refolding of bovine serum albumin. II. Evidence for the independent refolding of the domains of the molecule.

1976 • 64 citations

Refolding of bovine serum albumin and its proteolytic fragments. Regain of disulfide bonds, secondary structure, and ligand-binding ability.

1981 • 63 citations

Conformation of a stable intermediate on the folding pathway of Staphylococcus aureus penicillinase

1978 • 62 citations

Influence of ionic strength and pH on the interaction between high-affinity heparin and antithrombin

1981 • 58 citations

Binding to antithrombin of heparin fractions with different molecular weights

1981 • 53 citations

Comparison of Denaturation by Guanidine Hydrochloride of the Wild Type Tryptophan Synthase α-Subunit of Escherichia coli and Two Mutant Proteins (Glu 49—Met or Gln)

1979 • 53 citations

Active site of α1-antitrypsin: Homologous site in antithrombin-III

1980 • 50 citations

Studies on the binding of heparin to prothrombin and thrombin and the effect of heparin-binding on thrombin activity

1978 • 50 citations

GUANIDINE HYDROCHLORIDE AND THE CIRCULAR DICHROISM OF RANDOM COIL POLYPEPTIDES

1973 • 46 citations

pH dependence of stability of the wild-type tryptophan synthase α-subunit and two mutant proteins (Glu49 → Met or Gln)

1980 • 45 citations

The N‐terminal sequence of human plasma histidine‐rich glycoprotein homologous to antithrombin with high affinity for herapin

1982 • 43 citations

Evidence by Chemical Modification for the Involvement of One or More Tryptophanyl Residues of Bovine Antithrombin in the Binding of High‐Affinity Heparin

1979 • 39 citations

Demonstration of a two-domain structure of antithrombin III during its denaturation in guanidinium chloride.

1983 • 39 citations

Slow, spontaneous dissociation of the antithrombin—thrombin complex produces a proteolytically modified form of the inhibitor

1980 • 37 citations

Properties of antithrombin-thrombin complex formed in the presence and in the absence of heparin

1983 • 35 citations

Mechanism of inactivation of trypsin by antithrombin

1982 • 35 citations

Neutralization of heparin activity by binding to human histidine-rich glycoprotein

1983 • 29 citations

The different forms of antithrombin II in serum.

1977 • 27 citations

Resistance of soybean trypsin inhibitor (Kunitz) to denaturation by guanidinium chloride.

1977 • 22 citations

Occurrence and characterization of stable intermediate state(s) in the unfolding of ovomucoid by guanidine hydrochloride

1978 • 22 citations

Refolding properties of antithrombin III. Mechanism of binding to heparin.

1983 • 22 citations

Reconstitution of Lactic Dehydrogenase after Acid Dissociation

1981 • 22 citations

Simplex: A Method for Spectral Deconvolution Applicable to Energy Dispersion Analysis

1979 • 17 citations

Fractionation of heparin by chromatography on immobilized thrombin. Correlation between the anticoagulant activity of the fractions and their content of heparin with high affinity for antithrombin

1980 • 15 citations

The Denaturation of Muscle Phosphorylase b by Urea

1972 • 9 citations

Kinetics of thermal denaturation of antithrombin III

1982 • 7 citations