Proton NMR studies of T4 gene 32 protein: effects of zinc removal and reconstitution

SINGLE-STRANDED DNA BINDING PROTEINS REQUIRED FOR DNA REPLICATION

1986 • 563 citations

Gene 32 protein, the single-stranded DNA binding protein from bacteriophage T4, is a zinc metalloprotein.

1986 • 137 citations

Selectively deuterated amino acid analogues synthesis, Incorporation into proteins and NMR properties

1977 • 100 citations

Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities.

1979 • 98 citations

Proton NMR (500 MHz) of gene 32 protein-oligonucleotide complexes

1984 • 61 citations

T4 gene 32 protein trypsin-generated fragments. Fluorescence measurement of DNA-binding parameters.

1979 • 58 citations

The function of zinc in gene 32 protein from T4

1987 • 56 citations

Proton NMR (500 MHz) identification of aromatic residues of gene 32 protein involved in DNA binding by use of protein containing perdeuterated aromatic residues and by site-directed mutagenesis

1986 • 52 citations

Preparation of selectively deuterated aromatic amino acids for use in 1H NMR studies of proteins

1976 • 45 citations

Site-specific mutagenesis of T4 gene 32: the role of tyrosine residues in protein.cntdot.nucleic acid interactions

1989 • 40 citations

NMR spectroscopy of cadmium-113(II) substituted gene 32 protein

1989 • 36 citations

Cloning of T4 gene 32 and expression of the wild-type protein under lambda promoter PL regulation in Escherichia coli.

1986 • 33 citations

Thermal denaturation of T4 gene 32 protein: effects of zinc removal and substitution

1988 • 30 citations