Role of the carboxy-terminus of polypeptide D1 in the assembly of a functional water-oxidizing manganese cluster in photosystem II of the cyanobacterium Synechocystis sp. PCC 6803:assembly requires a free carboxyl group at C-terminal position 344

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

In vitro insertional mutagenesis with a selectable DNA fragment

1984 • 1,708 citations

Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b -559

1987 • 1,044 citations

[85] Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803

1988 • 975 citations

The Topology of the Plastoquinone and Herbicide Binding Peptides of Photosystem II in the Thylakoid Membrane

1986 • 487 citations

Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803

1989 • 305 citations

Directed alteration of the D1 polypeptide of photosystem II: evidence that tyrosine-161 is the redox component, Z, connecting the oxygen-evolving complex to the primary electron donor, P680

1989 • 296 citations

Kinetics of the oxidation—reduction reactions of the photosystem II quinone acceptor complex, and the pathway for deactivation

1983 • 266 citations

Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803.

1990 • 236 citations

Nanosecond reduction kinetics of photooxidized chlorophyll-aII (P-680) in single flashes as a probe for the electron pathway, H+-release and charge accumulation in the O2-evolving complex

1984 • 226 citations

Aspartate 170 of the photosystem II reaction center polypeptide D1 is involved in the assembly of the oxygen-evolving manganese cluster

1992 • 223 citations

Kinetics of reduction of the oxidized primary electron donor of Photosystem II in spinach chloroplasts and in Chlorella cells in the microsecond and nanosecond time ranges following flash excitation

1978 • 188 citations

Molecular architecture of the rapidly metabolized 32-kilodalton protein of photosystem II. Indications for COOH-terminal processing of a chloroplast membrane polypeptide.

1984 • 179 citations

COOH-terminal processing of polypeptide D1 of the photosystem II reaction center of Scenedesmus obliquus is necessary for the assembly of the oxygen-evolving complex.

1988 • 171 citations

Light-Induced Fluorescence Changes in Chlorella , and the Primary Photoreactions for the Production of Oxygen

1972 • 165 citations

Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis strain PCC 6803

1989 • 160 citations

Construction of an Obligate Photoheterotrophic Mutant of the Cyanobacterium Synechocystis 6803

1987 • 153 citations

Protein composition of the photosystem II core complex in genetically engineered mutants of the cyanobacterium Synechocystis sp. PCC 6803

1988 • 152 citations

The topology of a membrane protein: The orientation of the 32 kd Qb-binding chloroplast thylakoid membrane protein

1986 • 143 citations

The iron‐quinone electron‐acceptor complex of photosystem II

1991 • 138 citations

Changes in electrophoretic mobility of a chloroplast membrane polypeptide associated with the loss of the oxidizing side of photosystem II in low fluorescent mutants of Scenedesmus

1980 • 119 citations

The kinetics of light-induced changes of C-550, cytochrome b559 and fluorescence yield in chloroplasts at low temperature

1973 • 112 citations

Optical characterization of the immediate electron donor to chlorophyll a⁺_II in O₂‐evolving photosystem II complexes Tyrosine as possible electron carrier between chlorophyll a_II and the water‐oxidizing manganese complex

1988 • 111 citations

Chiloroplast gene for M_r32000 polypeptide of photosystem II InEuglena gracilisis interrupted by four Introns with conserved boundary sequences

1984 • 111 citations

Molecular cloning of the genes encoding two chaperone proteins of the cyanobacterium Synechocystis sp. PCC 6803.

1991 • 106 citations

New evidence supporting energy transfer between photosynthetic units

1973 • 98 citations

Identification of psbA and psbD gene products, D1 and D2, as reaction centre proteins of photosystem 2

1987 • 98 citations

Evidence from directed mutagenesis that aspartate 170 of the D1 polypeptide influences the assembly and/or stability of the manganese cluster in the photosynthetic water-splitting complex

1992 • 97 citations

psbG Is Not a Photosystem Two Gene but May Be an ndh Gene

1989 • 96 citations

Glu-69 of the D2 protein in photosystem II is a potential ligand to Mn involved in photosynthetic oxygen evolution

1990 • 96 citations

Processing of a Chloroplast-Translated Membrane Protein in vivo. Analysis of the Rapidly Synthesized 32000-dalton Shield Protein and Its Precursor in Spivodefa oligorrhiza

1982 • 91 citations

Site-directed mutagenesis of photosynthetic reaction centers

1991 • 86 citations

COOH‐terminal residues of D1 and the 44 kDa CPa‐2 at spinach photosystem II core complex

1988 • 82 citations

Processing of the D1 polypeptide of the photosystem II reaction centre and photoactivation of a low fluorescence mutant (LF‐1) of Scenedesmus obliquus

1988 • 70 citations

Lack of photoactivation capacity in Scenedesmus obliquus LF-1 results from loss of half the high-affinity manganese-binding site

1989 • 66 citations

Expression of a higher plant psbA gene in Synechocystis 6803 yields a functional hybrid photosystem II reaction center complex.

1991 • 59 citations

Structure of the Euglena gracilis chloroplast gene (psbA) coding for the 32‐kDa protein of Photosystem II

1984 • 57 citations

The carboxyl-terminal extension of the D1 protein of photosystem II is not required for optimal photosynthetic performance under CO2- and light-saturated growth conditions.

1992 • 55 citations

The high-affinity binding site for manganese on the oxidizing side of Photosystem II

1987 • 53 citations

Effect of signal sequence alterations on export of levansucrase in Bacillus subtilis

1991 • 44 citations

Affinity Chromatography on Immobilized Anhydrotrypsin: General Utility for Selective Isolation of C-Terminal Peptides from Protease Digests of Proteins1

1987 • 41 citations

The carboxyl modifier 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC) inhibits half of the high-affinity manganese-binding site in photosystem II membrane fragments

1991 • 41 citations

Nucleotide sequence of a secondpsbAgene from the unicellular cyanobacteriumSynechocystis6803

1989 • 41 citations

The rate of reduction of oxidized redox-active tyrosine, Z+, by exogenous Mn2+ is slowed in a site-directed mutant, at aspartate 170 of polypeptide D1 of photosystem II, inactive for photosynthetic oxygen evolution

1992 • 39 citations

Truncation of the carboxy-terminal domain of the psbE gene product in Synechocystis sp. PCC 6803: requirements for photosystem II assembly and function

1992 • 34 citations

The carboxy-terminal extension of the D1-precursor protein is dispensable for a functional photosystem II complex in Chlamydomonas reinhardtii

1992 • 34 citations

Residues flanking the COOH-terminal C-region of a model eukaryotic signal peptide influence the site of its cleavage by signal peptidase and the extent of coupling of its co-translational translocation and proteolytic processing in vitro.

1990 • 33 citations

Characterisation of the D1 protein in a photosystem II mutant (LF‐1) of Scenedesmus obliquus blocked on the oxidising side Evidence supporting non‐processing of D1 as the cause of the lesion

1988 • 32 citations

Chromatographic Purification and Determination of the Carboxy-Terminal Sequences of Photosystem II Reaction Center Proteins, Dl and D2

1990 • 32 citations

Protease treatments of photosystem II membrane fragments reveal that these are four separate high-affinity manganese-binding sites

1991 • 27 citations

New Results about Chlorophyll Fluorescence “In Vivo”

1972 • 27 citations

Nucleotide sequence of thepsbA3gene from the cyanobacteriumSynechocystisPCC 6803

1990 • 27 citations

Primary structure of a 7Fe ferredoxin from Streptomyces griseus

1990 • 26 citations

Detection of a Precursor Polypeptide of the Rapidly-Synthesized 32,000-Dalton Thylakoid Protein in Spinach Chloroplasts

1985 • 22 citations

A Synechocystis PCC 6803 psbA Deletion Mutant and Its Transformation with a psbA Gene from a Higher Plant

1990 • 19 citations

Regeneration of the high-affinity manganese-binding site in the reaction center of an oxygen-evolution deficient mutant of Scenedesmus by protease action

1989 • 9 citations