Nucleic acid selection as a tool for drug discovery

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

1990 • 9,690 citations

In vitro selection of RNA molecules that bind specific ligands

1990 • 9,093 citations

Selection of single-stranded DNA molecules that bind and inhibit human thrombin

1992 • 2,361 citations

High-Resolution Molecular Discrimination by RNA

1994 • 1,126 citations

Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures

1992 • 798 citations

DIVERSITY OF OLIGONUCLEOTIDE FUNCTIONS

1995 • 794 citations

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

1990 • 527 citations

Directed Evolution of an RNA Enzyme

1992 • 451 citations

RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase.

1992 • 422 citations

Mirror-image RNA that binds D-adenosine

1996 • 378 citations

HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA

1991 • 376 citations

RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity

1996 • 361 citations

High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I.

1996 • 350 citations

An enzyme-linked oligonucleotide assay

1996 • 330 citations

Molecular Recognition of Amino Acids by RNA-Aptamers: An L-Citrulline Binding RNA Motif and Its Evolution into an L-Arginine Binder

1994 • 280 citations

Nuclease-resistant nucleic acid ligands to vascular permeability factor/vascular endothelial growth factor

1995 • 276 citations

Oligonucleotides as Research, Diagnostic, and Therapeutic Agents

1995 • 262 citations

Selexion

1991 • 252 citations

In vitro selection from protein and peptide libraries

1994 • 242 citations

Expression of small, therapeutic RNAs in human cell nuclei

1997 • 225 citations

Specific binding of aminoglycoside antibiotics to RNA

1995 • 213 citations

RNA aptamers specifically interact with the prion protein PrP

1997 • 212 citations

Potent 2'-Amino-2'-deoxypyrimidine RNA Inhibitors of Basic Fibroblast Growth Factor

1995 • 210 citations

Bioactive and nuclease-resistant <scp>l</scp> -DNA ligand of vasopressin

1997 • 202 citations

Mirror-design of L-oligonucleotide ligands binding to L-arginine

1996 • 194 citations

In vitro selection of RNA aptamers specific for cyanocobalamin

1994 • 193 citations

The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing -(1 -pentynyl)-2′-deoxyuridine

1994 • 192 citations

Evolution in vitro of an RNA enzyme with altered metal dependence

1993 • 191 citations

A novel RNA motif for neomycin recognition

1995 • 182 citations

Inhibition of receptor binding by high-affinity RNA ligands to vascular endothelial growth factor

1994 • 179 citations

Modified RNA sequence pools forin vitroselection

1994 • 175 citations

High-affinity ssDNA inhibitors of the reverse transcriptase of type 1 human immunodeficiency virus

1995 • 165 citations

Use of a High Affinity DNA Ligand in Flow Cytometry

1996 • 161 citations

In vivo imaging of inflammation using an aptamer inhibitor of human neutrophil elastase

1997 • 157 citations

High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding.

1993 • 145 citations

From oligonucleotide shapes to genomic SELEX: Novel biological regulatory loops

1997 • 137 citations

Libraries for genomic SELEX

1997 • 136 citations

Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope.

1996 • 133 citations

DNA aptamers block L-selectin function in vivo. Inhibition of human lymphocyte trafficking in SCID mice.

1996 • 131 citations

Using in vitro selection to direct the covalent attachment of human immunodeficiency virus type 1 Rev protein to high-affinity RNA ligands.

1995 • 130 citations

Isolation of virus-neutralizing RNAs from a large pool of random sequences.

1995 • 119 citations

In vitro selection of RNA lectins: using combinatorial chemistry to interpret ribozyme evolution

1995 • 119 citations

The secondary structure and sequence optimization of an RNA ligase ribozyme

1995 • 118 citations

In Vitro Selection of RNA Ligands to Substance P

1995 • 117 citations

An RNA pocket for an aliphatic hydrophobe

1994 • 117 citations

Selection of an RNA domain that binds Zn2+.

1995 • 107 citations

RNA Selection: Aptamers achieve the desired recognition

1994 • 106 citations

Tagged versus untagged libraries: methods for the generation and screening of combinatorial chemical libraries.

1994 • 106 citations

Isozyme-specific inhibition of protein kinase C by RNA aptamers.

1994 • 106 citations

Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies

1997 • 96 citations

Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor.

1995 • 87 citations

Localization of the single-stranded DNA binding site in the thrombin anion-binding exosite.

1992 • 79 citations

In vitro and in vivo characterization of novel mRNA motifs that bind special elongation factor SelB

1997 • 76 citations

RNA aptamers selected to bind human immunodeficiency virus type 1 Rev in vitro are Rev responsive in vivo

1996 • 74 citations

Inhibition of cell proliferation by an RNA ligand that selectively blocks E2F function

1996 • 72 citations

In vitro selection of RNA-based irreversible inhibitors of human neutrophil elastase

1995 • 71 citations

Protective effects of an aptamer inhibitor of neutrophil elastase in lung inflammatory injury

1997 • 66 citations

Peptide conjugation to an in vitro-selected DNA ligand improves enzyme inhibition.

1995 • 60 citations

Selection of a Suppressor Mutation That Restores Affinity of an Oligonucleotide Inhibitor for Thrombin Using in Vitro Genetics

1995 • 60 citations

Small RNA-divalent domains.

1996 • 58 citations

Comprehensive Chemical Modification Interference and Nucleotide Substitution Analysis of an RNA Pseudoknot Inhibitor to HIV-1 Reverse Transcrip tase

1995 • 56 citations

Highly Potent Irreversible Inhibitors of Neutrophil Elastase Generated by Selection from a Randomized DNA−Valine Phosphonate Library

1997 • 56 citations

genetic analysis: Selection and amplification of rare functional nucleic acids

1991 • 54 citations

Dissecting protein:protein interactions between transcription factors with an RNA aptamer.

1995 • 40 citations

Detecting Immobilized Protein Kinase C Isozymes with RNA Aptamers

1996 • 39 citations

Isolation of a nuclease-resistant decoy RNA that selectively blocks autoantibody binding to insulin receptors on human lymphocytes.

1996 • 39 citations

The use of phage display in neurobiology

1995 • 27 citations

Defining a Smaller RNA Substrate for Elongation Factor Tu

1995 • 22 citations

Screening chemical libraries for nucleic-acid-binding drugs by in vitro selection: A test case with lividomycin

1996 • 19 citations

Natural and unnatural answers to evolutionary questions

1997 • 11 citations

Using in vitro nucleic acid selections for conventional drug design

1994 • 8 citations