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Determination of isoelectric points in thin-layer isoelectric focusing: The importance of attaining the steady state and the role of CO2 interference

Data up to Jan 2025

Published1978
Citations56
References33

Total Citations Per Year

Abstract

References (33)

Isoelectric points and molecular weights of proteins

1976 • 520 citations

Isoelectric focusing in layers of granulated gels

1973 • 280 citations

Electrostatic effects in myoglobin. Hydrogen ion equilibriums in sperm whale ferrimyoglobin

1974 • 211 citations

Electrofocusing and Isotachophoresis

1977 • 203 citations

Progress in isoelectric focusing and isotachophoresis

1976 • 171 citations

HISTORICAL AND THEORETICAL ASPECTS OF ISOELECTRIC FOCUSING*

1973 • 150 citations

MICROHETEROGENEITY AND ALLOMORPHISM OF PROTEINS

1973 • 134 citations

Isoelectric focusing of proteins in the native and denatured states. Anomalous behaviour of plasma albumin

1971 • 126 citations

Studies on Brassica seed proteins. I. The low molecular weight proteins in rapeseed. Isolation and characterization.

1972 • 112 citations

SMALL‐SCALE FRACTIONATION OF PROTEINS AND NUCLEIC ACIDS BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS*

1973 • 111 citations

Four Isoperoxidases from Horse Radish Root.

1970 • 110 citations

Thin-layer isoelectric focusing of proteins

1969 • 108 citations

INSTABILITY OF pH GRADIENTS FORMED BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GEL

1973 • 94 citations

Studies on Brassica seed proteins

1972 • 93 citations

The isolation and characterization of the glycopeptides from horseradish peroxidase isoenzyme C

1976 • 86 citations

On the Heterogeneity of Beef Heart Cytochrome c. IV. Isoelectric Fractionation by Electrolysis in a Natural pH-Gradient.

1966 • 82 citations

Artifacts in isoelectric focusing

1970 • 80 citations

Thin-layer isoelectric focusing on Sephadex layers of horseradish peroxidase

1970 • 75 citations

Polyacrylamide gel isoelectric focusing of proteins: Determination of isoelectric points using an antimony electrode

1972 • 71 citations

PHYSICOCHEMICAL PROPERTIES OF THE CARRIER AMPHOLYTES AND SOME BIOCHEMICAL APPLICATIONS*

1973 • 61 citations

Isoelectric Focusing and Separation of the Subcomponents of Lactoperoxidase.

1967 • 55 citations

Fractionation of Horseradish Peroxidase by Preparative Isoelectric Focusing, Gel Chromatography and Ion‐Exchange Chromatography

1975 • 46 citations

The hydrogen ion equilibria of horseradish peroxidase and apoperoxidase

1971 • 43 citations

Thin-layer isoelectric focusing of multiple forms of tomato pectinesterase

1976 • 41 citations

Detection of peroxidase by the print technique in thin-layer isoelectric focusing

1972 • 34 citations

Free electrofocusing in a coil of polyethylene tubing

1970 • 26 citations

Comparison of different techniques for isoelectric focusing on polyacrylamide gel slabs using bacterial asparaginases

1972 • 24 citations

Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. VI. Isoelectric Spectrum of Bovine Carbonic Anhydrase B.

1968 • 21 citations

THE USE OF GEL ISOELECTROFOCUSING IN MONITORING CHEMICAL MODIFICATIONS OF PROTEINS*

1973 • 17 citations

Amino Acid Sequence Studies of Horseradish Peroxidase. I. Tryptic Peptides

1972 • 15 citations

Structural Damage of Gamma-irradiated Ribonuclease Revealed by Thin-layer Isoelectric Focusing

1975 • 15 citations

SEPARATION OF AUSTRALIA ANTIGEN AND SOME BACTERIAL PROTEINS BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS*

1973 • 12 citations

Charge fluctuations in isoionic protein solutions. Determination of the quantity S = (¯z2)½ by means of conductivity measurements and hydrogen ion titrations

1969 • 8 citations

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Determination of isoelectric points in thin-layer isoelectric focusing: The importance of… (1978) – Analytical Biochemistry | Metascience Observatory Explorer