ß-Lactamases: Molecular Studies

The Journal of General Microbiology

1985 • 2,446 citations

Novel Method for Detection of β-Lactamases by Using a Chromogenic Cephalosporin Substrate

1972 • 1,857 citations

The structure of β-lactamases

1980 • 1,705 citations

Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.

1965 • 943 citations

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

1978 • 791 citations

The Use of Analytical Isoelectric Focusing for Detection and Identification of -Lactamases

1975 • 705 citations

PENICILLIN-BINDING PROTEINS AND THE MECHANISM OF ACTION OF BETA-LACTAM ANTIBIOTICS

1983 • 698 citations

Clavulanic Acid: a Beta-Lactamase-Inhibiting Beta-Lactam from Streptomyces clavuligerus

1977 • 695 citations

Estimation of Molecular Size and Molecular Weights of Biological Compounds by Gel Filtration

1970 • 635 citations

The β-lactamases of Gram-negative bacteria and their rôle in resistance to β-lactam antibiotics

1976 • 539 citations

The chemistry of penicillin

1949 • 408 citations

ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.

1981 • 384 citations

Organization of transcriptional signals in plasmids pBR322 and pACYC184.

1981 • 365 citations

Antimicrobial Agents and Chemotherapy-1961

1962 • 314 citations

The generation of a colE1-Apr cloning vehicle which allows detection of inserted DNA

1975 • 288 citations

Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia

1982 • 234 citations

Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

1982 • 234 citations

Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

1975 • 231 citations

.beta.-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin

1980 • 222 citations

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

1982 • 211 citations

Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro.

1982 • 210 citations

Chemistry of Penicillin

1949 • 203 citations

Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

1980 • 203 citations

Expression of human fibroblast interferon gene in Escherichia coli

1980 • 190 citations

Genome Construction Between Bacterial Species In Vitro: Replication and Expression of Staphylococcus Plasmid Genes in Escherichia coli

1974 • 176 citations

Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility

1976 • 172 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

Clavulanic acid, a novel β-lactam isolated from Streptomyces clavuligerus; X-ray crystal structure analysis

1976 • 160 citations

Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium

1981 • 160 citations

Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.

1978 • 159 citations

Trapping of nonhydrolyzable cephalosporins by cephalosporinases in Enterobacter cloacae and Pseudomonas aeruginosa as a possible resistance mechanism

1982 • 157 citations

Chromosomal beta-lactamases of Enterobacter cloacae are responsible for resistance to third-generation cephalosporins

1983 • 154 citations

Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis

1981 • 154 citations

Evidence for posttranslational translocation of β-lactamase across the bacterial inner membrane

1982 • 153 citations

Identification of -Lactamases by Analytical Isoelectric Focusing: Correlation with Bacterial Taxonomy

1976 • 145 citations

Membrane-bound penicillinases in Gram-positive bacteria.

1982 • 142 citations

Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence.

1982 • 131 citations

Penicillinase active sites: Labelling of serine‐44 in β‐lactamase I by 6β‐bromopenicillanic acid

1979 • 129 citations

The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.

1984 • 128 citations

Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria

1982 • 124 citations

On the process of cellular division in Escherichia coli: Nucleotide sequence of the gene for penicillin-binding protein 3

1983 • 123 citations

Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 Å resolution

1982 • 123 citations

Diverse effects of mutations in the signal sequence on the secretion of β-lactamase in Salmonella typhimurium

1982 • 121 citations

Molecular cloning and expression of Bacillus licheniformis beta-lactamase gene in Escherichia coli and Bacillus subtilis.

1981 • 120 citations

Staphylococcal penicillinase and the new penicillins

1962 • 111 citations

Sequence elements determining ampC promoter strength in E. coli.

1982 • 110 citations

Inactivation of the RTEM .beta.-lactamase from Escherichia coli. Interaction of penam sulfones with the enzyme

1981 • 110 citations

The nucleotide sequences of the ponA and ponB genes encoding penicillin‐binding proteins 1A and 1B of Escherichia coli K12

1985 • 110 citations

Occurrence of a serine residue in the penicillin‐binding site of the exocellular DD‐carboxy‐peptidase‐transpeptidase from Streptomyces R61

1976 • 108 citations

Penicillanic acid sulfone: an unexpected isotope effect in the interaction of 6.alpha.- and 6.beta.-monodeuterio and of 6,6-dideuterio derivatives with RTEM .beta.-lactamase from Escherichia coli. Crystal structure of penicillanic acid sulfone

1981 • 107 citations

Use of Model Enzymes in the Determination of the Mode of Action of Penicillins and Delta3-Cephalosporins

1979 • 106 citations

Inactivation of Bacillus cereus .beta.-lactamase I by 6.beta.-bromopenicillanic acid: kinetics

1980 • 105 citations

The acyl-enzyme mechanism of β-lactamase action. The evidence for class C β-lactamases

1982 • 96 citations

Purification and properties of the cellulases from the thermophilic fungus Thermoascus aurantiacus

1980 • 94 citations

Products of aminolysis and enzymic hydrolysis of the cephalosporins

1970 • 94 citations

Sequence of active site peptides from the penicillin-sensitive D-alanine carboxypeptidase of Bacillus subtilis. Mechanism of penicillin action and sequence homology to beta-lactamases.

1980 • 89 citations

Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae.

1981 • 87 citations

Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.

1982 • 84 citations

The production and molecular properties of the zinc β-lactamase of Pseudomonas maltophilia IID 1275

1985 • 82 citations

Penicillin Target Enzyme and the Antibiotic Binding Site

1982 • 81 citations

Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coli.

1981 • 81 citations

Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

1982 • 81 citations

Substituent effects upon the base hydrolysis of penicillins and cephalosporins. Competitive intramolecular nucleophilic amino attack in cephalosporins

1974 • 80 citations

β-Lactamase inactivation by mechanism-based reagents

1980 • 79 citations

Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.

1981 • 76 citations

R-Factor-Mediated β-Lactamases That Hydrolyze Oxacillin: Evidence for Two Distinct Groups

1974 • 75 citations

Diffusion-limited component of reactions catalyzed by Bacillus cereus .beta.-lactamase I

1984 • 74 citations

Active sites of β-lactamases. The chromosomal β-lactamases of Pseudomonas aeruginosa and Escherichia coli

1982 • 72 citations

Conformation of penicillin as a transition-state analog of the substrate of peptidoglycan transpeptidase

1971 • 70 citations

Inhibition of the RTEM .beta.-lactamase from Escherichia coli. Interaction of the enzyme with derivatives of olivanic acid

1982 • 70 citations

Directed selective pressure on a β-lactamase to analyse molecular changes involved in development of enzyme function

1976 • 69 citations

Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule.

1980 • 67 citations

Purification and Some Properties of a Cephalosporinase from Proteus vulgaris

1981 • 67 citations

Inactivation of radiolabeled RTEM .beta.-lactamase from Escherichia coli by clavulanic acid and 9-deoxyclavulanic acid

1981 • 67 citations

Elimination of a good leaving group from the 3'-position of a cephalosporin need not be concerted with .beta.-lactam ring opening: TEM-2 .beta.-lactamase-catalyzed hydrolysis of pyridine-2-azo-4'-(N',N'-dimethylaniline) cephalosporin (PADAC) and of cephaloridine

1984 • 66 citations

Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus beta-lactamase gene, in Bacillus subtilis

1984 • 63 citations

Single amino acid mutations block a late step in the folding of β-lactamase from Staphylococcus aureus

1985 • 62 citations

Identification of a novel ampC beta-lactamase promoter in a clinical isolate of Escherichia coli.

1982 • 60 citations

beta-Lactamase-catalyzed hydrolysis of acyclic depsipeptides and acyl transfer to specific amino acid acceptors.

1984 • 60 citations

Characterization of eight beta-lactamases of Gram-negative bacteria

1982 • 59 citations

Interaction of clavulanate with the β-lactamases of Streptomyces albus G and Actinomadura R39

1982 • 56 citations

Comparison of the overlapping frd and ampC operons of Escherichia coli with the corresponding DNA sequences in other gram-negative bacteria

1983 • 56 citations

Inactivation of Bacillus cereus .beta.-lactamase I by 6.beta.-bromopenicillanic acid: mechanism

1980 • 55 citations

Penicillanic acid sulfone: interaction with RTEM .beta.-lactamase from Escherichia coli at different pH values

1981 • 53 citations

Molecular cloning and nucleotide sequence of the type I β-lactamase gene fromBacillus cereus

1983 • 53 citations

The inhibition of β-lactamases from Gram-negative bacteria by clavulanic acid

1981 • 52 citations

The pH-dependence and group modification of β-lactamase I

1975 • 51 citations

Common evolutionary origin of chromosomal beta-lactamase genes in enterobacteria

1982 • 50 citations

A mecillinam-sensitive peptidoglycan crosslinking reaction in

1982 • 50 citations

Properties of three carbenicillin-hydrolysing β-lactamases (CARB) from Pseudomonas aeruginosa: identification of a new enzyme

1981 • 50 citations

Molecular cloning of the gene for the β-lactamase of Bacillus licheniformis and its expression in Escherichia coli

1980 • 49 citations

The Complete Amino Acid Sequence of the Zn²⁺‐Containing <scp>d</scp>‐Alanyl‐<scp>d</scp>‐Alanine‐Cleaving Carboxypeptidase of Streptomyces albus G

1983 • 49 citations

Model catalysts which stimulate penicillinase. V. The cycloheptaamylose-catalyzed hydrolysis of penicillins

1971 • 46 citations

STAPHYLOCOCCAL PENICILLINASE I

1961 • 45 citations

Cloning arg3, the gene for ornithine carbamoyltransferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid beta-lactamase in yeast.

1981 • 45 citations

The Classification and Terminology of Enzymes That Hydrolyze -Lactam Antibiotics

1982 • 45 citations

Interaction of β-iodopenicillanate with the β-lactamases of Streptomyces albus G and Actinomadura R39

1982 • 44 citations

A comparison of the antibacterial and β-lactamase inhibiting properties of penam and (2,3)-β-methylenepenam derivatives

1983 • 44 citations

.beta.-Lactamase III of Bacillus cereus 569: membrane lipoprotein and secreted protein

1983 • 43 citations

Purification and Properties of Chromosomally Mediated -Lactamase from Citrobacter freundii GN7391

1980 • 42 citations

Identification of the signal peptidase cleavage site in Bacillus licheniformis prepenicillinase.

1982 • 39 citations

The dimeric nature of an R-factor mediated β-lactamase

1976 • 39 citations

Pre-steady state beta-lactamase kinetics. Observation of a covalent intermediate during turnover of a fluorescent cephalosporin by the beta-lactamase of STaphylococcus aureus PC1.

1981 • 38 citations

Correlation Between Hydrolysis of the β-Lactam Bond of the Cephalosporin Nucleus and Expulsion of the 3-Substituent

1972 • 37 citations

Molecular Cloning and Expression of Penicillinase Genes from Bacillus licheniformis in the Thermophile Bacillus stearothermophilus

1982 • 36 citations

Identification of histidine residues that act as zinc ligands in β-lactamase II by differential tritium exchange

1979 • 35 citations

Construction of penP delta 1, Bacillus licheniformis 749/C beta-lactamase lacking site for lipoprotein modification. Expression in Escherichia coli and Bacillus subtilis.

1983 • 35 citations

Plasmid-directed expression of Staphylococcus aureus beta-lactamase by Bacillus subtilis in vitro.

1981 • 34 citations

Pre-steady state beta-lactamase kinetics. The trapping of a covalent intermediate and the interpretation of pH rate profiles.

1983 • 33 citations

5.5 Å crystallographic structure of penicillin β-lactamase and radius of gyration in solution

1976 • 33 citations

Transcription of the bacterial β-lactamase gene in Saccharomyces cerevisiae

1982 • 32 citations

Crystallographic data for the β-lactamase from Enterobacter cloacae P99

1983 • 32 citations

Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive <scp>d</scp>-alanyl-<scp>d</scp>-alanine-cleaving transpeptidase

1982 • 31 citations

A Model System to Demonstrate That -Lactamase-Associated Antibiotic Trapping Could Be a Potential Means of Resistance

1983 • 30 citations

Transcriptional analyses of theBacillus licheniformis penPgene

1982 • 30 citations

Mechanism of inactivation of TEM-1 β-lactamase by 6-acetylmethylenepenicillanic acid

1983 • 29 citations

Isolation of a covalent intermediate in β‐lactamase I catalysis

1982 • 28 citations

Comparison of transcription of beta-lactamase genes specified by various ampicillin transposons

1982 • 27 citations

Characterization of the membrane .beta.-lactamase in Bacillus cereus 569/H/9

1983 • 27 citations

Production of a variant of β-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9

1980 • 26 citations

Bacillus cereus 569/H β‐lactamase I: Cloning in Escherichia coli and signal sequence determination

1983 • 26 citations

Some Properties of the Clostridium butyricum Group -Lactamase

1981 • 24 citations

Properties of cephalosporinase from Proteus morganii.

1981 • 24 citations

The exocellular β-lactamase of Streptomyces albus G. Purification, properties and comparison with the exocellular <scp>dd</scp>-carboxypeptidase

1981 • 24 citations

Catalysis of hydrolysis and aminolysis of benzylpenicillin mediated by a ternary complex with zinc ion and Tris(hydroxymethyl)aminomethane

1982 • 24 citations

On the chemistry of β-lactamase inhibition by 6β-bromopenicillanic acid

1980 • 24 citations

SEQUENCE OF PENICILLIN-BINDING PROTEIN 5 OF ESCHERICHIA COLI

1983 • 24 citations

Preliminary crystallographic data for β-lactamase I from Bacillus cereus 569

1978 • 23 citations

Purification and biochemical properties of beta-lactamase produced by Proteus rettgeri

1980 • 22 citations

THE RANGE AND SIGNIFICANCE OF VARIATIONS AMONGST BACTERIAL PENICILLINASES

1968 • 22 citations

The penicillin-binding site in the exocellular <scp>dd</scp>-carboxypeptidase-transpeptidase of Actinomadura R39

1981 • 21 citations

The crystal structure of β-lactamase from Staphylococcus aureus at 0.5 nm resolution

1985 • 21 citations

Expression of antibiotic resistance genes from Escherichia coli in Bacillus subtilis

1983 • 20 citations

pH Dependence and solvent deuterium oxide kinetic isotope effects on Bacillus cereus .beta.-lactamase I catalyzed reactions

1984 • 19 citations

6 beta-Iodopenicillanic acid (UI-38,006), a beta-lactamase inhibitor that extends the antibacterial spectrum of beta-lactam compounds: initial bacteriological characterization

1981 • 18 citations

Active site of staphylococcal beta-lactamase.

1980 • 18 citations

.BETA.-Lactamase inhibitory activities and synergistic effects of 5,6-cis-carbapenem antibiotics.

1982 • 16 citations

Phenylpropynal, a specific, irreversible, non-beta-lactam inhibitor of beta-lactamases.

1980 • 16 citations

Purification and properties of the exocellular β-lactamase of a Actinomadura strain R39

1982 • 16 citations

Inactivation of Bacillus cereus 569/H β-lactamase I by 6-β-(trifluoromethane sulfonyl)amidopenicillanic acid sulfone and its N-methyl derivative

1983 • 15 citations

Transfer of the chromosomal bla gene from Enterobacter cloacae to Escherichia coli by RP4::mini-Mu

1984 • 15 citations

Interactions between non-classical β-lactam compounds and the β-lactamases of Actinomadura R39 and Streptomyces albus G

1981 • 14 citations

The penicillinase of Bacillus licheniformis is an outer membrane protein in Escherichia coli

1983 • 14 citations

Improved purification and characterization of the OXA-2 β-lactamase

1984 • 12 citations

Crystallization and preliminary X-ray data for the exocellular β-lactamase of Bacillus licheniformis 749/C

1985 • 11 citations

Tertiary and secondary structure analysis of penicillin-binding proteins.

1980 • 9 citations

The phasmid as a tool for plasmid genetics: I. Fine structure of the β-lactamase gene

1982 • 9 citations

N-(functionalized alkyl) derivatives of 6-aminopenicillanic acid: A new series of specific inhibitors of .BETA.-lactamase from Enterobacter cloacae P99.

1983 • 9 citations

β-Lactamase—a human enzyme, too

1982 • 7 citations

Bacillus cereus 569/H penicillinase serine-44 acylation by diazotized 6-aminopenicillanic acid

1983 • 7 citations

Inactivation of β-lactamase I from B. cereus569H with phenylglyoxal, an arginine-selective reagent

1982 • 7 citations

Anomalous pH dependence of the reactions of carbenicillin and sulbenicillin with Bacillus cereus .beta.-lactamase I. Influence of the .alpha.-substituent charge on the kinetic parameters

1984 • 6 citations

Intra-and inter-molecular general base catalysis in the aminolysis of benzylpenicillin

1976 • 6 citations

Studies on the primary structures of the exocellular d-alanyl-d-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39

1981 • 6 citations

Products and mechanism of the oxyanion- and .beta.-lactamase-catalyzed hydrolyses of (phenoxymethyl)anhydropenicillin

1983 • 6 citations

Irreversible inactivation of yeast glucose-6-P dehydrogenase by penicillin G

1981 • 3 citations

Do intermolecular association phenomena occur in B. cereus beta-lactamase I?

1982 • 2 citations