Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning

Sequences of proteins of immunological interest

1984 • 3,427 citations

Somatic generation of antibody diversity

1983 • 3,275 citations

Predominant Autoantibody Production by Early Human B Cell Precursors

2003 • 1,966 citations

Structure of the human immunoglobulin μ locus: Characterization of embryonic and rearranged J and D genes

1981 • 959 citations

An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus

2004 • 705 citations

Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.

1993 • 691 citations

Defective B cell tolerance checkpoints in systemic lupus erythematosus

2005 • 677 citations

Energy crops: current status and future prospects

2006 • 488 citations

Autoreactivity in Human IgG+ Memory B Cells

2007 • 458 citations

Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction.

1995 • 434 citations

The Complete Nucleotide Sequence of the Human Immunoglobulin Heavy Chain Variable Region Locus

1998 • 401 citations

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

1997 • 373 citations

Unmutated and mutated chronic lymphocytic leukemias derive from self-reactive B cell precursors despite expressing different antibody reactivity

2005 • 331 citations

The use of laboratory tests in the diagnosis of SLE

2000 • 320 citations

Evolution of human immunoglobulin kappa J region genes.

1982 • 318 citations

Impaired early B cell tolerance in patients with rheumatoid arthritis

2005 • 313 citations

Contribution of Receptor Editing to the Antibody Repertoire

2001 • 312 citations

Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development

1993 • 311 citations

Sequence of the human immunoglobulin diversity (D) segment locus: a systematic analysis provides no evidence for the use of DIR segments, inverted D segments, “minor” D segments or D-D recombination 1 1Edited By J. Karn

1997 • 300 citations

High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis

1980 • 284 citations

Human monoclonal antibody production

1987 • 224 citations

A checkpoint for autoreactivity in human IgM+ memory B cell development

2006 • 209 citations

One-megabase sequence analysis of the human immunoglobulin lambda gene locus.

1997 • 209 citations

Epstein-Barr virus susceptibility of normal human B lymphocyte populations.

1984 • 193 citations

Ordering of Human Bone Marrow B Lymphocyte Precursors by Single-Cell Polymerase Chain Reaction Analyses of the Rearrangement Status of the Immunoglobulin H and L Chain Gene Loci

1996 • 186 citations

Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells using mixed primers and the polymerase chain reaction

1989 • 172 citations

Surrogate Light Chain Expressing Human Peripheral B Cells Produce Self-reactive Antibodies

2003 • 144 citations

Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes.

1992 • 142 citations

Persistent expression of autoantibodies in SLE patients in remission

2006 • 140 citations

Bruton's Tyrosine Kinase Is Essential for Human B Cell Tolerance

2004 • 138 citations

CD40 ligand and MHC class II expression are essential for human peripheral B cell tolerance

2007 • 130 citations

Human monoclonal antibodies produced by primary in vitro immunization of peripheral blood lymphocytes.

1988 • 121 citations

The variable genes of the human immunoglobulin kappa locus.

1993 • 120 citations

Immunoglobulin kappa chain receptor editing in systemic lupus erythematosus.

1998 • 111 citations

IgM Heavy Chain Complementarity-Determining Region 3 Diversity Is Constrained by Genetic and Somatic Mechanisms Until Two Months After Birth

1999 • 109 citations

Human Autoantibody Silencing by Immunoglobulin Light Chains

2004 • 101 citations

High-frequency representation of a single VH gene in the expressed human B cell repertoire.

1993 • 83 citations

Human immunoglobulin variable region gene analysis by single cell RT-PCR

2000 • 78 citations

Ig lambda and heavy chain gene usage in early untreated systemic lupus erythematosus suggests intensive B cell stimulation.

1999 • 66 citations

Ig λ and Heavy Chain Gene Usage in Early Untreated Systemic Lupus Erythematosus Suggests Intensive B Cell Stimulation

1999 • 52 citations

Monoclonal and recombinant antibodies, 30 years after …

2006 • 43 citations

Analysis of human V H gene repertoire expression in peripheral CD19+ B cells

1995 • 36 citations

Epstein-Barr virus preferentially induces proliferation of primed B cells.

1989 • 29 citations

Construction of representative immunoglobulin variable region cDNA libraries from human peripheral blood lymphocytes without in vitro stimulation

1991 • 25 citations

Use of Immunoglobulin Variable-Region Genes by Normal Subjects and Patients with Systemic Lupus Erythematosus

2000 • 21 citations

The selection and characterization of human monoclonal antibodies to human cytomegalovirus

1986 • 17 citations

VH gene-family representation in peripheral activated B cells from systemic lupus erythematosus (SLE) patients

1996 • 12 citations

Immunoglobulin heavy chain variable region gene usage in human autoimmune diseases

1995 • 7 citations