Reconstitution of ATPase activity from the isolated α, β, and γ subunits of the coupling factor, F1, of Escherichia coli
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Membranes and Energy Transduction in Bacteria1 1Abbreviations: Δψ, membrane potential; ΔpH, pH gradient; Δp, proton-motive force. These are related by: Δp = Δψ - (23RT/F) ΔpH ≅ Δψ - 60 ΔpH. ANS, l-anilino-8-naphthalene sulfonate; DCCD, N, N'-dicyclohexylcarbodiimide; CCCP, carbonylcyanide-m-chlorophenylhydrazone; HOQNO, hydroxyquinoline-N-oxide; PEP, phosphoenolpyruvic acid. EDTA, ATP, GTP, DNA, NAD(H), and NADP(H) have their usual meanings.
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