Precolumn derivatization for chromatographic and electrophoretic analyses of carbohydrates

High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl5-pyrazolone derivatives

1989 • 627 citations

Demonstration of peptide:N-glycosidase F activity in endo-beta-N-acetylglucosaminidase F preparations.

1984 • 588 citations

Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.

1975 • 432 citations

Analyses of N-linked oligosaccharides using a two-dimensional mapping technique

1988 • 390 citations

The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device

1990 • 293 citations

Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound

1978 • 287 citations

Simultaneous determination of reducing monosaccharides by capillary zone electrophoresis as the borate complexes of N-2-pyridylglycamines

1989 • 255 citations

Quantitative Determination of Monosaccharides in Glycoproteins by High-Performance Liquid Chromatography with Highly Sensitive Fluorescence Detection

1994 • 242 citations

Structural characterization of oligosaccharides by high-performance liquid chromatography, fast-atom bombardment-mass spectrometry, and exoglycosidase digestion

1984 • 193 citations

High-performance liquid chromatography of mono- and oligosaccharides

1984 • 184 citations

Separation of neutral oligosaccharides by high-performance liquid chromatography

1981 • 169 citations

[13] High-performance liquid chromatography of pyridylaminated saccharides

1994 • 162 citations

Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography

1985 • 150 citations

Analysis of the oligosaccharides in ovalbumin by high-performance capillary electrophoresis

1990 • 132 citations

Polyacrylamide gel electrophoresis of reducing saccharides labeled with the fluorophore 2-aminoacridone: Subpicomolar detection using an imaging system based on a cooled charge-coupled device

1991 • 123 citations

Capillary electrophoresis of amino sugars with laser-induced fluorescence detection

1991 • 119 citations

Structure of a Sugar Chain of a Protease Inhibitor Isolated from Barbados Pride (Caesalpinia pulcherrima Sw.) Seeds

1986 • 111 citations

Derivatization Procedures for Reducing Oligosaccharides, Part 3: Preparation of Oligosaccharide Glycosylamines, and Their Conversion Into Glycosaccharide - Acrylamide Copolymers

1989 • 110 citations

The asparagine-linked sugar chains of subcomponent C1q of the first component of human complement.

1978 • 105 citations

Release of O-Linked Sugar Chains from Glycoproteins with Anhydrous Hydrazine and Pyridylamination of the Sugar Chains with Improved Reaction Conditions1

1992 • 103 citations

Carbohydrate analysis-high-performance liquid chromatography and capillary electrophoresis

1995 • 101 citations

Capillary zone electrophoresis and micellar electrokinetic chromatography of 4‐aminobenzonitrile carbohydrate derivatives

1994 • 100 citations

Separation of Complex Oligosaccharide Mixtures by Capillary Electrophoresis in the Open-Tubular Format

1994 • 96 citations

Capillary zone electrophoresis of pyridylamino derivatives of maltooligosaccharides

1990 • 93 citations

A Highly Sensitive Method for Analyses of Sugar Moieties of Glycoproteins by Fluorescence Labeling

1981 • 93 citations

Determination of amino sugars and amino acids in glycoconjugates using precolumn derivatization with o-phthalaldehyde

1992 • 89 citations

Sensitive, laser-assisted determination of complex oligosaccharide mixtures separated by capillary gel electrophoresis at high resolution

1992 • 88 citations

Carbohydrate analysis : high performance liquid chromatography and capillary electrophoresis

1995 • 86 citations

The Analysis of Fluorophore-Labeled Glycans by High-Resolution Polyacrylamide-Gel Electrophoresis

1994 • 86 citations

The Structure of the Aerobacter aerogenes A3(S1) Polysaccharide. II. Sequence Analysis and Hydrolysis Studies^*

1966 • 85 citations

Reversed-phase liquid chromatographic analysis of dns-sugars

1983 • 84 citations

Comparative study of the mucin-type sugar chains of human chorionic gonadotropin present in the urine of patients with trophoblastic diseases and healthy pregnant women.

1988 • 82 citations

[31] Glycosyltransferase assays using pyridylaminated acceptors: N-acetylglucosaminyltransferase III, IV, and V

1989 • 79 citations

Microdetermination of Individual Neutral and Amino Sugars and N-Acetylneuraminic Acid in Complex Saccharides<xref ref-type="fn" rid="fn1"><sup>*</sup></xref>

1974 • 79 citations

Determination of Monosaccharides in Glycoproteins by Reverse-Phase High-Performance Liquid Chromatography

1993 • 78 citations

Synthesis and application of Fmoc-hydrazine for the quantitative determination of saccharides by reversed-phase high-performance liquid chromatography in the low and subpicomole range

1991 • 73 citations

Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars

1977 • 73 citations

Three-dimensional mapping of N-linked oligosaccharides using anion-exchange, hydrophobic and hydrophilic interaction modes of high-performance liquid chromatography

1996 • 72 citations

A new method for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates

1991 • 70 citations

Application of 2- Aminopyridine Fluorescence Labeling to Glycosaminoglycans1

1991 • 69 citations

Parameterization of contribution of sugar units to elution volumes in reverse-phase HPLC of 2-pyridylaminated oligosaccharides

1990 • 68 citations

Structures of asparagine-linked oligosaccharides from hen egg-yolk antibody (IgY). Occurrence of unusual glucosylated oligo-mannose type oligosaccharides in a mature glycoprotein

1991 • 68 citations

Precolumn labeling of reducing carbohydrates with 1-(p-methoxy)phenyl-3-methyl-5-pyrazolone: Analysis of neutral and sialic acid-containing oligosaccharides found in glycoproteins

1991 • 66 citations

Electrophoretic resolution and fluorescence detection of N-linked glycoprotein oligosaccharides after reductive amination with 8-aminonaphthalene-1,3,6-trisulphonic acid

1992 • 66 citations

Detection of 100 molecules of product formed in a fucosyltransferase reaction

1994 • 65 citations

Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins

1987 • 60 citations

Resolution of the branched forms of oligosaccharides by high-performance capillary electrophoresis

1994 • 60 citations

Qualitative and quantitative analysis of reducing carbohydrates by radiochromatography on ion-exchange papers

1973 • 57 citations

Estimation of elution times on reverse-phase high-performance liquid chromatography of pyridylamino derivatives of sugar chains from glycoproteins

1990 • 55 citations

Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released from glycosphingolipids by endoglycoceramidase

1990 • 53 citations

New derivatization and separation procedures for reducing oligosaccharides

1986 • 52 citations

High expression of an N-acetylglucosaminyltransferase III in 3′-methyl DAB-induced hepatoma and ascites hepatoma

1988 • 51 citations

Studies on the Substrate Specificity of Neutral α-Mannosidase Purified from Japanese Quail Oviduct by Using Sugar Chains from Glycoproteins1

1991 • 48 citations

Pre-column derivatisation in high-performance liquid chromatography

1977 • 44 citations

Analysis of Sugar Chains by Pyridylamination

2003 • 44 citations

Analysis of oligosaccharides by on-line high-performance liquid chromatography and ion-spray mass spectrometry

1992 • 42 citations

Separation of aminated monosaccharides by capillary zone electrophoresis with laser-induced fluorescence detection

1994 • 41 citations

Sensitive method for carbohydrate composition analysis of glycoproteins by high-performance liquid chromatography

1987 • 41 citations

Simplified Approach to HPLC Precolumn Fluorescent Labeling of Carbohydrates: N-(2-Pyridinyl)-glycosylamnes

1987 • 37 citations

Quantitative determination of phenyl isothiocyanate-derivatized amino sugars and amino sugar alcohols by high-performance liquid chromatography

1991 • 37 citations

High-performance liquid chromatographic analysis of galactosamine, glucosamine, glucosaminitol, and galactosaminitol

1987 • 37 citations

High-performance reversed-phase chromatographic mapping of 2-pyridylamino derivatives of xyloglucan oligosaccharides

1991 • 35 citations

Two-dimensional mapping by high-performance liquid chromatography of pyridylamino oligosaccharides from various glycosphingolipids

1991 • 35 citations

The Analysis of Mixtures of Closely Related Naturally-occurring Organic Compounds Using High Performance Liquid Chromatography

1974 • 31 citations

Improved Method for the Component Sugar Analysis of Glycoproteins by Pyridylamino Sugars Purified with Immobilized Boronic Acid

1992 • 30 citations

Studies on the Sugar Chains of Interferon-γ from Human Peripheral-Blood Lymphocytes1

1989 • 28 citations

Pyridylamino Sugar Chain as an Acceptor for Galactosyltransferase1

1988 • 28 citations

Monosaccharide determination of glycoconjugates by reverse-phase high-performance liquid chromatography of their phenylthiocarbamyl derivatives

1992 • 25 citations

Identification and derivatization of (oligosaccharyl)amines obtained by treatment of asparagine-linked glycopeptides with N-GLYCANASE enzyme

1992 • 25 citations

n-Alkyl p-aminobenzoates as derivatizing agents in the isolation, separation, and characterization of submicrogram quantities of oligosaccharides by liquid secondary ion mass spectrometry

1991 • 25 citations

Electrospray ionization mass spectrometry of pyridylaminated oligosaccharide derivatives: Sensitivity and in-source fragmentation

1994 • 24 citations

Derivatization Procedures for Reducing Oligosaccharides, Part 4: Use of Glycosylamines in a Reversible Derivatization of Oligosaccharides with the 9-Fluorenylmethoxycarbonyl Group, and Hplc Separations of the Derivatives

1991 • 23 citations

Ionophoresis of Oligosaccharides as N-Benzylglycosylammonium Ions

1956 • 22 citations

A sensitive and selective method for the analysis of complex mixtures of sugars and linear oligosaccharides

1994 • 21 citations

Femtomole oligosaccharide detection using a reducing-end derivative and chemical ionization mass spectrometry

1990 • 21 citations

Tyrosine Derivatization and Preparative Purification of the Sialyl and Asialyl N-Linked Oligosaccharides from Porcine Fibrinogen

1994 • 21 citations

Conversion of Pyridylamino Sugar Chains to 1-Amino-1-Deoxy Derivatives, Intermediates for Tagging with Fluorescein and Biotin

1992 • 19 citations

¹H‐NMR analysis of the sugar structures of glycoproteins as their pyridylamino derivatives

1986 • 19 citations

Derivatization procedures for reducing oligosaccharides, part 2: Chemical transformation of 1-deoxy-1-(4-trifluoroacetamidophenyl)aminoalditols

1988 • 18 citations

Functional Fluorescence Labeling of Carbohydrates and Its Use for Preparation of Neoglycoconjugates

1994 • 16 citations

The use of polyacrylamide gel electrophoresis for the analysis of acidic glycans labeled with the fluorophore 2‐aminoacridone

1994 • 14 citations

Tritiated borohydride reduction of carbohydrates: reduction of individual monosaccharides, alone or in groups, results in highly divergent specific activities

1992 • 10 citations

High-performance liquid chromatographic separation of monosaccharides as their peracetylated ketoximes and aldononitriles

1990 • 10 citations

N,N-(2,4-Dinitrophenyl)octylamine Derivatives for the Isolation, Purification, and Mass Spectrometric Characterization of Oligosaccharides

1993 • 9 citations