Coupling between local structure and global stability of a protein: mutants of staphylococcal nuclease

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

[6] Statistical analysis of enzyme kinetic data

1979 • 2,002 citations

A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study

1974 • 1,384 citations

Consideration of the possibility that the slow step in protein denaturation reactions is due to cis-trans isomerism of proline residues

1975 • 1,108 citations

Protein stability curves

1987 • 990 citations

On the use of sequence homologies to predict protein structure: identical pentapeptides can have completely different conformations.

1984 • 386 citations

Staphylococcal nuclease: Proposed mechanism of action based on structure of enzyme—thymidine 3′,5′-bisphosphate—calcium ion complex at 1.5-Å resolution

1979 • 375 citations

How Do Enzymes Work?

1988 • 359 citations

Metachromatic Agar-Diffusion Methods for Detecting Staphylococcal Nuclease Activity

1971 • 328 citations

The Thermodynamics of Protein Denaturation. I. The Denaturation of Chymotrypsinogen

1964 • 328 citations

GENETIC ANALYSIS OF STAPHYLOCOCCAL NUCLEASE: IDENTIFICATION OF THREE INTRAGENIC "GLOBAL" SUPPRESSORS OF NUCLEASE-MINUS MUTATIONS

1985 • 189 citations

Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis

1987 • 168 citations

Proline isomerism in staphylococcal nuclease characterized by NMR and site-directed mutagenesis

1987 • 167 citations

Location of proline derivatives in conformational space. I. Conformational calculations; optical activity and NMR experiments

1970 • 153 citations

Stability mutants of staphylococcal nuclease: large compensating enthalpy-entropy changes for the reversible denaturation reaction

1988 • 149 citations

Cold denaturation of staphylococcal nuclease.

1988 • 147 citations

A magnetization-transfer nuclear magnetic resonance study of the folding of staphylococcal nuclease

1989 • 133 citations

Kinetic and magnetic resonance studies of effects of genetic substitution of a calcium-liganding amino acid in staphylococcal nuclease

1986 • 122 citations

Staphylococcal nuclease reviewed: A prototypic study in contemporary enzymology

1979 • 65 citations

Two cis‐prolines in the Bence‐Jones protein Rei and the cis‐pro‐bend

1974 • 64 citations

Thermal denaturation of staphylococcal nuclease

1985 • 57 citations

Hydrogen-1 NMR evidence for three interconverting forms of staphylococcal nuclease: effects of mutations and solution conditions on their distribution

1989 • 48 citations

Staphylococcal Nuclease: Size and Specificity of the Active Site

1968 • 47 citations

Staphylococcal Nuclease (Foggi Strain)

1971 • 43 citations

Evidence for cis peptide bonds in copolypeptides of glycine and proline

1972 • 43 citations

Two-dimensional NMR studies of Staphylococcal nuclease. 1. Sequence-specific assignments of hydrogen-1 signals and solution structure of the nuclease H124L-thymidine 3',5'-bisphosphate-calcium ternary complex

1990 • 42 citations

NMR assignments of the four histidines of staphylococcal nuclease in native and denatured states

1988 • 40 citations

Identification of residues involved in a conformational change accompanying substitutions for glutamate-43 in staphylococcal nuclease

1988 • 38 citations

Chymotrypsinogen family of proteins. XI. Heat-capacity changes accompanying reversible thermal unfolding of proteins

1971 • 33 citations

Introduction of a Metal-dependent Regulatory Switch into an Enzyme

1989 • 28 citations

Staphylococcal nuclease active-site amino acids: pH dependence of tyrosines and arginines by carbon-13 NMR and correlation with kinetic studies

1989 • 23 citations