Expression of the cystic fibrosis transmembrane conductance regulator gene in cells of non-epithelial origin

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Genomic sequencing.

1984 • 8,343 citations

Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA

1989 • 7,327 citations

Identification of the Cystic Fibrosis Gene: Genetic Analysis

1989 • 4,105 citations

Update on Key Emerging Challenges in Cystic Fibrosis

2014 • 3,606 citations

Identification of the Cystic Fibrosis Gene: Chromosome Walking and Jumping

1989 • 3,180 citations

Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene

1984 • 3,150 citations

Therapy of CF-Patients with Amitriptyline and Placebo - a Randomised, Double-Blind, Placebo-Controlled Phase IIb Multicenter, Cohort-Study

2013 • 2,504 citations

Chromosomal Subunits in Active Genes Have an Altered Conformation

1976 • 1,842 citations

Isolation of Lymphocytes, Granulocytes and Macrophages

1976 • 1,621 citations

Isolation and Characterization of Full-Length cDNA Clones for Human α-, β-, and γ-Actin mRNAs: Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed

1983 • 1,220 citations

The Relation between Genotype and Phenotype in Cystic Fibrosis — Analysis of the Most Common Mutation (ΔF₅₀₈)

1990 • 724 citations

Illegitimate transcription: transcription of any gene in any cell type.

1989 • 647 citations

Altered Regulation of Airway Epithelial Cell Chloride Channels in Cystic Fibrosis

1986 • 568 citations

Generation of cAMP-Activated Chloride Currents by Expression of CFTR

1991 • 550 citations

Cystic fibrosis: a disease in electrolyte transport

1990 • 510 citations

Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance

1991 • 508 citations

Abnormal Ion Permeation Through Cystic Fibrosis Respiratory Epithelium

1983 • 493 citations

Cyclic AMP-dependent protein kinase opens chloride channels in normal but not cystic fibrosis airway epithelium

1988 • 419 citations

A cluster of cystic fibrosis mutations in the first nucleotide-binding fold of the cystic fibrosis conductance regulator protein

1990 • 397 citations

Chloride and potassium channels in cystic fibrosis airway epithelia

1986 • 392 citations

Separate Cl- conductances activated by cAMP and Ca2+ in Cl(-)-secreting epithelial cells.

1990 • 360 citations

Multiple mutations in highly conserved residues are found in mildly affected cystic fibrosis patients

1990 • 353 citations

Cl ^- Channels in CF: Lack of Activation by Protein Kinase C and cAMP-Dependent Protein Kinase

1989 • 267 citations

A cystic fibrosis pancreatic adenocarcinoma cell line.

1990 • 237 citations

Abnormal regulation of ion channels in cystic fibrosis epithelia

1990 • 236 citations

Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis.

1991 • 230 citations

Regulation of Chloride Channels by Protein Kinase C in Normal and Cystic Fibrosis Airway Epithelia

1989 • 229 citations

Accurate quantification of cells recovered by bronchoalveolar lavage.

1984 • 202 citations

Expression of the neutrophil elastase gene during human bone marrow cell differentiation.

1989 • 190 citations

The cystic fibrosis gene has a “housekeeping”-type promoter and is expressed at low levels in cells of epithelial origin

1991 • 170 citations

Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli.

1985 • 167 citations

A cAMP-Regulated Chloride Channel in Lymphocytes That Is Affected in Cystic Fibrosis

1989 • 163 citations

Two Patients with Cystic Fibrosis, Nonsense Mutations in Each Cystic Fibrosis Gene, and Mild Pulmonary Disease

1990 • 146 citations

Variable deletion of exon 9 coding sequences in cystic fibrosis transmembrane conductance regulator gene mRNA transcripts in normal bronchial epithelium.

1991 • 138 citations

Myelomonocytic cell lineage expression of the neutrophil elastase gene.

1988 • 119 citations

A frame-shift mutation in the cystic fibrosis gene

1990 • 107 citations

Cell Cycle Dependence of Chloride Permeability in Normal and Cystic Fibrosis Lymphocytes

1990 • 94 citations

Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester

1991 • 94 citations

Gene expression in murine erythroleukemia cells

1984 • 78 citations

Cystic fibrosis and beta-adrenergic response of airway epithelial cell cultures

1986 • 71 citations

Phosphorylation‐activated chloride channels in human skin fibroblasts

1988 • 50 citations

Increased eIF-2 alpha expression in mitogen-activated primary T lymphocytes.

1990 • 43 citations

Identification of a defective cAMP-stimulated Cl- channel in cystic fibrosis fibroblasts.

1987 • 36 citations

Complementary endeavours

1990 • 27 citations

Secretion of antileucoprotease from a human lung tumor cell line

1987 • 18 citations

Proton pumps and chloride channels in secretory vesicles.

1989 • 5 citations