Increased analytical sensitivity of RT-PCR of PSA mRNA decreases diagnostic specificity of detection of prostatic cells in blood

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Prostate Specific Antigen: A Critical Assessment of the Most Useful Tumor Marker for Adenocarcinoma of the Prostate

1991 • 1,273 citations

Expression of the prostate-specific membrane antigen.

1994 • 582 citations

Detection of major bcr-abl gene expression at a very low level in blood cells of some healthy individuals

1995 • 481 citations

Detection of hematogenous micrometastasis in patients with prostate cancer.

1992 • 374 citations

Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay

1994 • 320 citations

Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction.

1994 • 271 citations

Sensitive nested reverse transcription polymerase chain reaction detection of circulating prostatic tumor cells: comparison of prostate-specific membrane antigen and prostate-specific antigen-based assays.

1994 • 241 citations

Haematogenous dissemination of prostatic epithelial cells during radical prostatectomy

1995 • 225 citations

Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: clinical implications.

1995 • 220 citations

Detection of circulating tumor cells in men with localized prostate cancer.

1994 • 180 citations

Detection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase chain reaction.

1993 • 152 citations

Prostate-specific antigen messenger RNA is expressed in non-prostate cells: implications for detection of micrometastases.

1995 • 142 citations

Primers are decisive for sensitivity of PCR.

1994 • 124 citations

Molecular Detection of Prostate Epithelial Cells from the Surgical Field and Peripheral Circulation during Radical Prostatectomy

1996 • 120 citations

Characterization of the gene for prostate-specific antigen, a human glandular kallikrein

1989 • 117 citations

Preoperative Reverse Transcriptase Polymerase Chain Reaction for Prostate Specific Antigen Predicts Treatment Failure Following Radical Prostatectomy

1996 • 103 citations

Enhanced detection of hematogenous circulating prostatic cells in patients with prostate adenocarcinoma by using nested reverse transcription polymerase chain reaction assay based on prostate-specific membrane antigen

1995 • 96 citations

Original Articles: Prostate Cancer: Measurement of Serum Prostate Specific Antigen Levels in Women and in Prostatectomized Men With an Ultrasensitive Immunoassay Technique

1995 • 90 citations

Molecular staging of prostate cancer. II. A comparison of the application of an enhanced reverse transcriptase polymerase chain reaction assay for prostate specific antigen versus prostate specific membrane antigen.

1995 • 83 citations

Sensitive Detection of Prostatic Hematogenous Tumor Cell Dissemination Using Prostate Specific Antigen and Prostate Specific Membrane-Derived Primers in the Polymerase Chain Reaction

1995 • 82 citations

Detection of prostatic cells in peripheral blood: correlation with serum concentrations of prostate-specific antigen

1995 • 77 citations

Sensitivity of immunohistochemistry and polymerase chain reaction in detecting prostate cancer cells in bone marrow.

1994 • 66 citations

Molecular Stating of Prostate Cancer. II. A Comparison of the Application of an Enhanced Reverse Transcriptase Polymerase Chain Reaction Assay for Prostate Specific Antigen Versus Prostate Specific Membrane Antigen

1995 • 63 citations

Detection of prostate-specific antigen mRNA and protein in breast tumors

1996 • 38 citations

New diagnostic applications and physiological functions of prostate specific antigen

1995 • 32 citations

Serum prostate-specific antigen measured in children from birth to age 18 years

1996 • 26 citations

Detection of prostate-specific antigen mRNA by reverse transcription polymerase chain reaction and time-resolved fluorometry

1995 • 23 citations