Monoclonal antibodies to human fibroblast procollagenase. Inhibition of enzymatic activity, affinity purification of the enzyme, and evidence for clustering of epitopes in the amino-terminal end of the activated enzyme

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Plasminogen Activators, Tissue Degradation, and Cancer

1985 • 2,584 citations

Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System

1971 • 2,019 citations

Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates

1980 • 1,991 citations

Separation of Blood Leucocytes, Granulocytes and Lymphocytes

1974 • 667 citations

Human fibroblast collagenase. Complete primary structure and homology to an oncogene transformation-induced rat protein.

1986 • 645 citations

Comparison of human stromelysin and collagenase by cloning and sequence analysis

1986 • 443 citations

Further studies on the activation of procollagenase, the latent precursor of bone collagenase. Effects of lysosomal cathepsin B, plasmin and kallikrein, and spontaneous activation

1977 • 389 citations

Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures

1977 • 387 citations

Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution

1982 • 246 citations

Prostaglandin regulation of macrophage collagenase production.

1977 • 230 citations

Biosynthesis of tissue inhibitor of metalloproteinases by human fibroblasts in culture. Stimulation by 12-O-tetradecanoylphorbol 13-acetate and interleukin 1 in parallel with collagenase.

1985 • 220 citations

Purification of human plasma α2 macroglobulin and α1 proteinase inhibitor using zinc chelate chromatography

1979 • 213 citations

Human skin fibroblast procollagenase: mechanisms of activation by organomercurials and trypsin

1983 • 208 citations

Detergent-activation of latent collagenase and resolution of its component molecules

1982 • 202 citations

Granulocyte collagenase: selective digestion of type I relative to type III collagen.

1977 • 201 citations

Human alveolar macrophages produce a fibroblast-like collagenase and collagenase inhibitor.

1985 • 199 citations

Distribution of urokinase-type plasminogen activator immunoreactivity in the mouse.

1984 • 181 citations

The activation of human skin fibroblast procollagenase. Sequence identification of the major conversion products.

1987 • 173 citations

Secreted forms of human neutrophil collagenase.

1986 • 167 citations

[8] Catabolism and turnover of collagens: Collagenases

1987 • 151 citations

Human fibroblast collagenase: glycosylation and tissue-specific levels of enzyme synthesis.

1986 • 125 citations

Synthesis of collagenase and collagenase inhibitors by osteoblast-like cells in culture

1984 • 125 citations

Synthesis and release of procollagenase by cultured fibroblasts.

1976 • 99 citations

The temperature-sensitive bond in human alpha 2-macroglobulin is the alkylamine-reactive site.

1980 • 96 citations

Latent and Active Human Polymorphonuclear Leukocyte Collagenases

1983 • 87 citations

Collagenase is a major gene product of induced rabbit synovial fibroblasts.

1984 • 81 citations

Identification of plasma kallikrein as an activator of latent collagenase in rheumatoid synovial fluid

1982 • 73 citations

Evidence for the Presence of a Latent Form of Collagenase in Human Rheumatoid Synovial Fluid

1972 • 63 citations

Heterogeneity among human collagenases demonstrated by monoclonal antibody that selectively recognizes and inhibits human neutrophil collagenase.

1984 • 61 citations

Human fibroblast collagenase contains an amino acid sequence homologous to the zinc-binding site of Serratia protease.

1987 • 56 citations

Degradation of type I collagen by rat mucosal keratinocytes. Evidence for secretion of a specific epithelial collagenase.

1987 • 53 citations

An improved procedure for high-sensitivity microsequencing: Use of aminoethyl aminopropyl glass beads in the beckman sequencer and the Ultrasphere ODS column for PTH amino acid identification

1981 • 51 citations

A sensitive collagenase assay using [3H]collagen labeled by reaction with pyridoxal phosphate and [3H]borohydride

1981 • 43 citations

A simple modification on the vacuum system of the Beckman automated sequencer to improve the efficiency of edman degradation

1980 • 39 citations

Synovial Collagenase and Joint Diseases: The Significancy of Latent Collagenase with Special Reference to Rheumatoid Arthritis

1973 • 38 citations

Primary structure of human alpha 2-macroglobulin. I. Isolation of the 26 CNBr fragments, amino acid sequence of 13 small CNBr fragments, amino acid sequence of methionine-containing peptides, and alignment of all CNBr fragments.

1984 • 34 citations