Reconstitution in vitro of RNase H activity by using purified N-terminal and C-terminal domains of human immunodeficiency virus type 1 reverse transcriptase.

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Complete nucleotide sequence of the AIDS virus, HTLV-III

1985 • 2,236 citations

Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus

1970 • 2,061 citations

Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of RNA Tumour Viruses

1970 • 1,992 citations

A detailed model of reverse transcription and tests of crucial aspects

1979 • 621 citations

Characterization of Highly Immunogenic p66/p51 as the Reverse Transcriptase of HTLV-III/LAV

1986 • 543 citations

Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.

1986 • 483 citations

Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus

1986 • 308 citations

Expression of soluble, enzymatically active, human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants.

1988 • 228 citations

Domain structure of the Moloney murine leukemia virus reverse transcriptase: mutational analysis and separate expression of the DNA polymerase and RNase H activities.

1988 • 206 citations

Identification and characterization of HIV-specific RNase H by monoclonal antibody.

1988 • 191 citations

Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity

1988 • 190 citations

Activity of purified biosynthetic proteinase of human immunodeficiency virus on natural substrates and synthetic peptides.

1989 • 166 citations

Point mutations in conserved amino acid residues within the C‐terminal domain of HIV‐1 reverse transcriptase specifically repress RNase H function

1989 • 165 citations

HIV-1 RT-associated ribonuclease H displays both endonuclease and 3′----5′ exonuclease activity.

1990 • 154 citations

Processing protease and reverse transcriptase from human immunodeficiency virus type I polyprotein in Escherichia coli

1988 • 151 citations

HIV-1 reverse transcriptase: crystallization and analysis of domain structure by limited proteolysis

1988 • 145 citations

Co-expression of the Subunits of the Heterodimer of HIV-1 Reverse Transcriptase in Escherichia coli

1989 • 127 citations

AIDS virus reverse transcriptase defined by high level expression in Escherichia coli.

1987 • 126 citations

Linker insertion mutagenesis of the human immunodeficiency virus reverse transcriptase expressed in bacteria: definition of the minimal polymerase domain.

1989 • 117 citations

Involvement of retrovirus reverse transcriptase-associated RNase H in the initiation of strong-stop (+) DNA synthesis and the generation of the long terminal repeat

1984 • 95 citations

Recombinant HIV-1 reverse transcriptase: Purification, primary structure, and polymerase/ribonuclease H activities

1989 • 92 citations

Characterization of human immunodeficiency virus type 1 reverse transcriptase by using monoclonal antibodies: role of the C terminus in antibody reactivity and enzyme function

1988 • 91 citations

Intrinsic properties of reverse transcriptase in reverse transcription

1989 • 90 citations

Mechanism of release of the avian retrovirus tRNATrp primer molecule from viral DNA by ribonuclease H during reverse transcription

1982 • 88 citations

Mutational analysis of the ribonuclease H activity of human immunodeficiency virus 1 reverse transcriptase

1990 • 81 citations

Mechanism of RNA primer removal by the RNase H activity of avian myeloblastosis virus reverse transcriptase

1984 • 77 citations

Ribonuclease H activities associated with viral reverse transcriptases are endonucleases.

1989 • 75 citations

Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase

1989 • 74 citations

Specificity of initiation of plus-strand DNA by Rous sarcoma virus

1984 • 65 citations

Immunologic and proteolytic analysis of HIV-1 reverse transcriptase structure

1990 • 62 citations

[5] Polymers coupled to agarose as stable and high capacity spacers

1974 • 55 citations

Identification of a human immunodeficiency virus-1 protease cleavage site within the 66,000 dalton subunit of reverse transcriptase

1990 • 53 citations

RNase H hydrolysis of the 5′ terminus of the avian sarcoma virus genome during reverse transcription

1978 • 49 citations

Comparison of the Human Immunodeficiency Virus Type 1 and 2 Proteases by Hybrid Gene Construction and trans-Complementation

1989 • 48 citations

Effects of small insertions on the RNA-dependent DNA polymerase activity of HIV-1 reverse transcriptase

1989 • 48 citations

High-level expression of self-processed HIV-1 protease in Escherichia coli using a synthetic gene

1989 • 47 citations

Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity

1988 • 41 citations

Analysis of the ribonuclease H activity of HIV-1 reverse transcriptase using RNA.cntdot.DNA hybrid substrates derived from the gag region of HIV-1

1989 • 27 citations

Endoribonucleolytic cleavage of RNA: Oligodeoxynucleotide hybrids by the ribonuclease H activity of HIV-1 reverse transcriptase

1990 • 14 citations