Production in Escherichia coli, purification and immunogenicity of acrosomal protein SP-10, a candidate contraceptive vaccine

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.

1987 • 263 citations

Chelating peptide-immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins.

1988 • 237 citations

Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.

1986 • 182 citations

Biochemical and Morphological Characterization of the Intra-Acrosomal Antigen SP-1O from Human Sperm1

1990 • 143 citations

Cloning and Sequencing of cDNAs Coding for the Human Intra-Acrosomal Antigen SP-101

1990 • 88 citations

Characterization of a Sperm-specific Nuclear Autoantigenic Protein. I. Complete Sequence and Homology with the Xenopus Protein, N1/N21

1990 • 62 citations

Cloning and sequencing of baboon and cynomolgus monkey intraacrosomal protein SP‐10: Homology with human SP‐10 and a mouse sperm antigen (MSA‐63)

1993 • 59 citations

Purification and Microsequencing of the Intra-Acrosomal Protein SP-10. Evidence that SP-10 Heterogeneity Results from Endoproteolytic Processes1

1992 • 36 citations