Human Hepatic and Lipoprotein Lipase: The Loop Covering the Catalytic Site Mediates Lipase Substrate Specificity

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

The Metabolic Basis of Inherited Disease

1990 • 4,327 citations

A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

1967 • 1,317 citations

Structure of human pancreatic lipase

1990 • 1,203 citations

A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex

1991 • 1,112 citations

Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides

1969 • 774 citations

Interfacial activation of the lipase–procolipase complex by mixed micelles revealed by X-ray crystallography

1993 • 666 citations

A specific apoprotein activator for lipoprotein lipase

1970 • 665 citations

Catalysis at the interface: the anatomy of a conformational change in a triglyceride lipase

1992 • 503 citations

Human Lipoprotein Lipase Complementary DNA Sequence

1987 • 484 citations

High density lipoprotein2. Relationship of the plasma levels of this lipoprotein species to its composition, to the magnitude of postprandial lipemia, and to the activities of lipoprotein lipase and hepatic lipase.

1987 • 383 citations

Cofactor activity of protein components of human very low density lipoproteins in the hydrolysis of triglycerides by lipoprotein lipase from different sources

1973 • 327 citations

The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family.

1987 • 300 citations

Characterization of proteoliposomes containing apoprotein A-I: a new substrate for the measurement of lecithin: cholesterol acyltransferase activity.

1982 • 300 citations

A one-tube plasmid DNA mini-preparation suitable for sequencing

1988 • 278 citations

Structure and evolution of the lipase superfamily.

1992 • 265 citations

Lipoprotein Metabolism during Acute Inhibition of Hepatic Triglyceride Lipase in the Cynomolgus Monkey

1982 • 259 citations

On the metabolic function of heparin-releasable liver lipase

1980 • 250 citations

Hepatic endothelial lipase antiserum influences rat plasma low and high density lipoproteins in vivo

1979 • 217 citations

Hydrolysis of human plasma high density lipoprotein2-phospholipids and triglycerides by hepatic lipase

1981 • 204 citations

Purification from human plasma of a heparin-released lipase with activity against triglyceride and phospholipids.

1975 • 204 citations

Lipoprotein lipase and hepatic lipase mRNA tissue specific expression, developmental regulation, and evolution.

1989 • 196 citations

Lipoprotein lipase. Molecular model based on the pancreatic lipase x-ray structure: consequences for heparin binding and catalysis.

1994 • 188 citations

Human hepatic lipase. Cloned cDNA sequence, restriction fragment length polymorphisms, chromosomal localization, and evolutionary relationships with lipoprotein lipase and pancreatic lipase.

1988 • 184 citations

Medium-chain vs long-chain triacylglycerol emulsion hydrolysis by lipoprotein lipase and hepatic lipase: implications for the mechanisms of lipase action

1990 • 182 citations

A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase

1993 • 172 citations

Lipoprotein lipase secretion by human monocyte-derived macrophages.

1982 • 166 citations

Lipoprotein metabolism in hepatic lipase deficiency: studies on the turnover of apolipoprotein B and on the effect of hepatic lipase on high density lipoprotein.

1988 • 155 citations

Large buoyant LDL-like particles in hepatic lipase deficiency.

1989 • 142 citations

Human lipoprotein lipase: the loop covering the catalytic site is essential for interaction with lipid substrates.

1992 • 130 citations

Plasma lipoproteins in familial hepatic lipase deficiency.

1990 • 126 citations

Horse Pancreatic Lipase

1994 • 118 citations

Catalytic triad residue mutation (Asp156—-Gly) causing familial lipoprotein lipase deficiency. Co-inheritance with a nonsense mutation (Ser447—-Ter) in a Turkish family

1991 • 117 citations

Role of lipoprotein lipase in the regulation of high density lipoprotein apolipoprotein metabolism. Studies in normal and lipoprotein lipase-inhibited monkeys.

1990 • 109 citations

Chimeras of hepatic lipase and lipoprotein lipase. Domain localization of enzyme-specific properties.

1992 • 107 citations

Human lipoprotein lipase. Analysis of the catalytic triad by site-directed mutagenesis of Ser-132, Asp-156, and His-241.

1992 • 104 citations

Positional Specificity of Lipoprotein Lipase

1972 • 100 citations

Activation of the Enzymic Activity of Hepatic Lipase by Apolipoprotein A‐II

1983 • 96 citations

Degradation of high density lipoprotein by heparin-releasable liver lipase

1980 • 95 citations

Different reactivities of high density lipoprotein2 subfractions with hepatic lipase.

1992 • 92 citations

Isolation and cDNA sequence of human postheparin plasma hepatic triglyceride lipase.

1988 • 90 citations

Structural and functional roles of highly conserved serines in human lipoprotein lipase. Evidence that serine 132 is essential for enzyme catalysis

1991 • 88 citations

Human hepatic triglyceride lipase: cDNA cloning, amino acid sequence and expression in a cultured cell line

1987 • 87 citations

Lipoprotein-lipase action determining plasma high density lipoprotein cholesterol level in adult normolipaemics

1980 • 77 citations

Domain exchange: characterization of a chimeric lipase of hepatic lipase and lipoprotein lipase.

1991 • 77 citations

Triacylglycerol and phospholipid hydrolysis in human plasma lipoproteins: role of lipoprotein and hepatic lipase

1992 • 77 citations

Lipoprotein lipaseBethesda: a single amino acid substitution (Ala-176----Thr) leads to abnormal heparin binding and loss of enzymic activity.

1990 • 77 citations

Role of apolipoprotein E in hepatic lipase-catalyzed hydrolysis of phospholipid in high-density lipoproteins

1992 • 70 citations

Hepatic lipase hydrolysis of lipid monolayers. Regulation by apolipoproteins.

1991 • 70 citations

Studies on the substrate specificity of purified human milk lipoprotein lipase

1982 • 63 citations

Hepatic lipase: site-directed mutagenesis of a serine residue important for catalytic activity.

1990 • 62 citations

Functional characterization of a chimeric lipase genetically engineered from human lipoprotein lipase and human hepatic lipase

1993 • 61 citations

Chymotryptic cleavage of lipoprotein lipase

1993 • 56 citations

The role of hepatic triglyceride lipase in the metabolism of intermediate-density lipoprotein—Postheparin lipolytic activities determined by a sensitive, nonradioisotopic method in hyperlipidemic patients and normals

1986 • 46 citations

Comparison of the action of lipoprotein lipase on triacylglycerols and phospholipids when presented in mixed liposomes or in emulsion droplets

1991 • 37 citations

Metabolism of apoB-100-containing lipoproteins in familial hyperchylomicronemia.

1993 • 34 citations

Activation of hepatic lipase catalyzed phosphatidylcholine hydrolysis by apolipoprotein E

1991 • 27 citations

Post-heparin plasma hepatic triacylglycerol lipase-catalyzed tributyrin hydrolysis Effect of trypsin treatment

1984 • 25 citations

Specificity of lipoprotein lipase and hepatic lipase toward monoacylglycerols varying in the acyl composition

1981 • 24 citations

Hydrolysis of neutral lipid substrates by rat hepatic lipase

1991 • 14 citations

Hepatic triacylglycerol lipase in circulating blood of normal and tumor-bearing mice and its hydrolysis of very-low-density lipoprotein and synthetic acylglycerols

1986 • 8 citations