A Novel Integration Signal That Is Composed of Two Transmembrane Segments Is Required to Integrate the Neurospora Plasma Membrane H+-ATPase into Microsomes

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

Intracellular protein topogenesis

1980 • 1,378 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

Location of high affinity Ca2 +-binding sites within the predicted transmembrahe domain of the sarco-plasmic reticulum Ca2+-ATPase

1989 • 569 citations

Nucleotide sequence of The yeastSUC2gene for invertase

1983 • 225 citations

Sequence analysis of mutations that prevent export of λ receptor, an Escherichia coli outer membrane protein

1980 • 195 citations

Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence

1988 • 185 citations

Conformational behavior of Escherichia coli OmpA signal peptides in membrane mimetic environments

1993 • 169 citations

Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.

1986 • 166 citations

Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.

1983 • 155 citations

Importance of secondary structure in the signal sequence for protein secretion.

1983 • 152 citations

Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase.

1986 • 124 citations

The affinity of signal recognition particle for presecretory proteins is dependent on nascent chain length.

1988 • 106 citations

Amino-terminal assembly of human P-glycoprotein at the endoplasmic reticulum is directed by cooperative actions of two internal sequences.

1993 • 86 citations

Structural requirements for membrane assembly of proteins spanning the membrane several times.

1989 • 79 citations

Solubilization and purification of the Neurospora plasma membrane H+-ATPase.

1981 • 73 citations

Construction of defined polytopic integral transmembrane proteins. The role of signal and stop transfer sequence permutations.

1988 • 71 citations

Membrane protein spanning segments as export signals

1992 • 69 citations

Peptide environment specifies conformation. Helicity of hydrophobic segments compared in aqueous, organic, and membrane environments.

1993 • 54 citations

[18] Slab gel system for the resolution of oligopeptides below molecular weight of 10,000

1983 • 38 citations

Identification of the membrane-embedded regions of the Neurospora crassa plasma membrane H(+)-ATPase

1991 • 38 citations

Requirement for conformational flexibility in the signal sequence of precursor protein.

1994 • 23 citations

Secretory protein translocation in a neurospora crassa in vitro system. Hydrolysis of a nucleoside triphosphate is required for posttranslational translocation.

1987 • 20 citations

Location of signal sequences for membrane insertion of the Na+,K+-ATPase alpha subunit.

1989 • 20 citations

Topology of the Neurospora plasma membrane H(+)-ATPase. Localization of a transmembrane segment.

1994 • 14 citations

GTP is required for the integration of a fragment of the Neurospora crassa H+-ATPase into homologous microsomal vesicles

1991 • 6 citations

Studies on the sedimentation behavior of the Neurospora crassa plasma membrane H+-ATPase synthesized in vitro and integrated into homologous microsomal membranes

1990 • 6 citations

The initial association of a truncated form of the Neurospora plasma membrane H+-ATPase and of the precursor of yeast invertase with microsomes are distinct processes

1993 • 3 citations