Efficient gene transfer into human umbilical vein endothelial cells allows functional analysis of the human tissue factor gene promoter

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIA

1973 • 6,904 citations

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis.

1989 • 1,199 citations

Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1.

1986 • 1,079 citations

Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells.

1984 • 944 citations

Electroporation for the efficient transfection of mammalian cells with DNA

1987 • 856 citations

High efficiency polyoma DNA transfection of chloroquine treated cells

1983 • 711 citations

Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.

1989 • 709 citations

Cultured human endothelial cells generate tissue factor in response to endotoxin.

1983 • 516 citations

Electroporation in ‘intracellular’ buffer increases cell survival

1992 • 460 citations

Lethal E. coli septic shock is prevented by blocking tissue factor with monoclonal antibody.

1991 • 428 citations

Lipopolysaccharide-mediated transcriptional activation of the human tissue factor gene in THP-1 monocytic cells requires both activator protein 1 and nuclear factor kappa B binding sites.

1991 • 373 citations

Initiation and regulation of tissue factor-dependent blood coagulation.

1992 • 287 citations

Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade

1989 • 217 citations

Tissue-factor coagulant activity of cultured human endothelial and smooth muscle cells and fibroblasts

1977 • 205 citations

The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester.

1990 • 180 citations

Tissue-factor coagulant activity of cultured human endothelial and smooth muscle cells and fibroblasts

1977 • 161 citations

Methods for preparation of media, supplements, and substrata for serum-free animal cell culture

1984 • 146 citations

Regulation of RNA polymerase II transcription

1993 • 97 citations

Evaluation of Relative Promoter Strength in Primary Hepatocytes Using Optimized Lipofection

1991 • 86 citations

Functional analysis of the human tissue factor promoter and induction by serum.

1990 • 84 citations

Lipid A as the biologically active moiety in bacterial endotoxin (LPS)- initiated generation of procoagulant activity by peripheral blood leukocytes

1977 • 70 citations

DEAE-dextran enhances electroporation of mammalian cells

1992 • 64 citations

Cationic lipid-mediated transfection of liver cels in primary culture

1992 • 63 citations

Sequences Upstream from the Mousec-mosOncogene May Function as a Transcription Termination Signal

1986 • 54 citations

Macrophage Procoagulants

1984 • 46 citations

Ets transcription factor binding site is required for positive and TNFα-Hnduced negative promoter regulation

1993 • 39 citations

Quantitation of transient gene expression after electroporation

1992 • 22 citations

The uptake and expression of the factor VIII and reporter genes by vascular cells

1992 • 14 citations