A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES

1957 • 63,238 citations

The plasma lecithin:cholesterol acyltransferase reaction

1968 • 2,415 citations

Fertilizing Capacity of Spermatozoa deposited into the Fallopian Tubes

1951 • 1,451 citations

The function of sterols in membranes

1976 • 1,330 citations

Methods in Mammalian Embryology

1972 • 1,295 citations

The culture of mouse embryos in vitro.

1971 • 965 citations

Observations on the Penetration of the Sperm into the Mammalian Egg

1951 • 907 citations

Mechanisms of Fertilization in Mammals

1981 • 782 citations

A protein cofactor of lecithin:Cholesterol acyltransferase

1972 • 722 citations

Receptor-mediated endocytosis: insights from the lipoprotein receptor system.

1979 • 701 citations

Cytolytic and membrane-perturbing properties of lysophosphatidylcholine

1979 • 594 citations

Human semen and fertility regulation in men

1976 • 589 citations

Calcium dependence of the acrosome reaction and activation of guinea pig spermatozoa

1974 • 587 citations

Determination of Lecithin: Cholesterol Acyltransfer in Human Blood Plasma

1971 • 559 citations

Sperm Capacitation and Fertilization in Mammals

1970 • 513 citations

Mechanism and control of animal fertilization

1983 • 478 citations

Localization of red cell membrane constituents

1973 • 431 citations

The Fusion of Biological Membranes

1970 • 397 citations

The effect of sterol structure on the permeability of lipomes to glucose, glycerol and Rb+

1972 • 354 citations

Modification of red cell membrane structure by cholesterol-rich lipid dispersions. A model for the primary spur cell defect.

1975 • 354 citations

Biochemistry of Mammalian Fertilization

1974 • 353 citations

Lysolecithin and Cell Fusion

1970 • 350 citations

MEMBRANE VESICULATION AS A FEATURE OF THE MAMMALIAN ACROSOME REACTION

1967 • 348 citations

The Effects of Estrogen Administration on Plasma Lipoprotein Metabolism in Premenopausal Females

1983 • 340 citations

The properties of polyunsaturated lecithins in monolayers and liposomes and the interactions of these lecithins with cholesterol

1972 • 339 citations

Mouse gamete interactions during fertilization in vitro. Chlortetracycline as a fluorescent probe for the mouse sperm acrosome reaction.

1979 • 331 citations

Observations on the penetration of sperm into the mammalian egg

1951 • 329 citations

Hepatic catabolism of rat and human lipoproteins in rats treated with 17 alpha-ethinyl estradiol.

1979 • 322 citations

The effect of partial replacements of membrane cholesterol by other steroids on the osmotic fragility and glycerol permeability of erythrocytes

1969 • 321 citations

Mouse gamete interactions: The zona pellucida is the site of the acrosome reaction leading to fertilization in vitro

1982 • 313 citations

Timing of fertilization in mammals: sperm cholesterol/phospholipid ratio as a determinant of the capacitation interval.

1981 • 310 citations

Analysis of Human Spermatozoal Fertilizing Ability Using Zona-Free Ova

1979 • 299 citations

High density lipoprotein distribution

1978 • 290 citations

Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock

1977 • 278 citations

Albumin-Deficient Rat Mutant

1979 • 277 citations

A Detrimental Effect of Seminal Plasma on the Fertilizing Capacity of Sperm

1957 • 270 citations

Familial Plasma Lecithin: Cholesterol Acyltransferase Deficiency Biochemical Study of a New Inborn Error of Metabolism

1967 • 267 citations

Ultrastructural changes in the sperm head during fertilization in the rabbit

1968 • 229 citations

An ultrastructural study of epididymal mouse spermatozoa binding to zonae pellucidae in vitro: Sequential relationship to the acrosome reaction

1979 • 224 citations

LYSOSOMAL ENZYMES IN THE ACROSOME AND THEIR POSSIBLE ROLE IN FERTILIZATION

1970 • 224 citations

Recent progress in the endocrinology of reproduction

1960 • 217 citations

Increased calcium-ion influx is a component of capacitation of spermatozoa

1978 • 214 citations

The mechanism of activation of lecithin:cholesterol acyltransferase by apolipoprotein A-I and an amphiphilic peptide.

1980 • 204 citations

The effects of subfractions of high density lipoprotein on cholesterol efflux from cultured fibroblasts. Regulation of low density lipoprotein receptor activity.

1981 • 201 citations

Proteins of human follicular fluid: the blood-follicle barrier.

1973 • 200 citations

The role of calcium in the acrosome reaction: An analysis using ionophore A23187

1976 • 197 citations

Evidence for a lipoprotein carrier in human plasma catalyzing sterol efflux from cultured fibroblasts and its relationship to lecithin:cholesterol acyltransferase.

1981 • 197 citations

Purification and properties of an anionic zymogen of phospholipase a from porcine pancreas

1968 • 189 citations

An electron microscopic study of sperm penetration into the rabbit egg after natural mating

1972 • 189 citations

Membrane particle changes attending the acrosome reaction in guinea pig spermatozoa

1977 • 187 citations

Effects of various lipids on the acrosome reaction and fertilizing capacity of guinea pig spermatozoa with special reference to the possible involvement of lysophospholipids in the acrosome reaction

1981 • 187 citations

Studies on the mechanism of capacitation II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albumin during capacitation in vitro

1979 • 185 citations

Lectin-binding sites on the plasma membranes of rabbit spermatozoa: Changes in surface receptors during epididymal maturation and after ejaculation

1977 • 183 citations

THE IMPORTANCE OF HYDROLYTIC ENZYMES TO AN EXOCYTOTIC EVENT, THE MAMMALIAN SPERM ACROSOME REACTION

1984 • 178 citations

OVIDUCAL AND UTERINE FLUIDS

1974 • 176 citations

The selective loss of lysophospholipids in some commonly used lipid-extraction procedures

1974 • 173 citations

Human Serum Lipoproteins and Their Interrelationships

1967 • 172 citations

Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide.

1976 • 170 citations

Proteins of Human Follicular Fluid: The Blood-Follicle Barrier

1973 • 169 citations

STUDIES ON THE ACROSOME. III. EFFECT OF CALCIUM DEFICIENCY

1954 • 168 citations

Lipids of the Red Cell Membrane

1974 • 168 citations

Production, characterization, and use of ionophore‐induced, calcium‐dependent acrosome reaction in ram spermatozoa

1981 • 166 citations

Studies on the mechanism of capacitation: albumin-mediated changes in plasma membrane lipids during in vitro incubation of rat sperm cells.

1980 • 164 citations

Chondroitin Sulfate Facilitates an Acrosome Reaction in Bovine Spermatozoa as Evidenced by Light Microscopy, Electron Microscopy and in Vitro Fertilization

1983 • 163 citations

Changes in the Electrophoretic Properties of Rabbit Spermatozoa During Passage Through the Epididymis

1963 • 154 citations

The status of acrosomal caps of hamster spermatozoa immediately before fertilization in vivo

1984 • 151 citations

Regional assignment of the steroid sulfatase—X-linked ichthyosis locus: Implications for a noninactivated region on the short arm of human X chromosome

1979 • 150 citations

Cholesterol, lipoproteins, apoproteins, and heart disease: present status and future prospects.

1981 • 148 citations

Membrane—membrane interactions

1980 • 146 citations

In vitro acrosome reaction and capacitation of golden hamster spermatozoa by bovine follicular fluid and its fractions

1969 • 144 citations

Changes of membrane permeability due to extensive cholesterol depletion in mammalian erythrocytes

1974 • 143 citations

The removal of cholesterol from landschütz ascites cells by high-density apolipoprotein

1973 • 142 citations

Analysis of membrane halves: cholesterol.

1976 • 141 citations

Radioimmunoassay of human plasma lecithin-cholesterol acyltransferase.

1981 • 141 citations

Localization of cholesteryl sulfate in human spermatozoa in support of a hypothesis for the mechanism of capacitation.

1981 • 135 citations

Polyunsaturated Fatty Acids in Relation to Sperm Motility

2009 • 135 citations

Studies on membrane fusion. 1. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide

1976 • 135 citations

Structural comparisons among glycosaminoglycans to promote an acrosome reaction in bovine spermatozoa

1982 • 129 citations

Influence of gonadal hormones on protein-lipid relationships in human plasma

1955 • 128 citations

Fertilization studies in the hamster *1The role of cell-surface carbohydrates

1982 • 126 citations

Evidence for the role of a trypsin‐like enzyme in the hamster sperm acrosome reaction

1976 • 124 citations

Familial Plasma Lecithin:Cholesterol Acyltransferase Deficiency

1969 • 122 citations

Thermodynamics of the salting effect; free energies, enthalpies, entropies, heat capacities, and volumes of the ternary systems electrolyte–alcohol–water at 25 °C

1978 • 121 citations

Autoradiographic localization of the sites of uptake, cellular transport, and catabolism of low density lipoproteins in the liver of normal and estrogen-treated rats

1981 • 118 citations

THE PHOSPHOLIPID COMPOSITION OF HUMAN SPERMATOZOA AND SEMINAL PLASMA

1973 • 118 citations

Jelly coat and induction of the acrosome reaction in echinoid sperm

1981 • 117 citations

Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin

1971 • 116 citations

Further evidence in support of a role for hamster sperm hydrolytic enzymes in the acrosome reaction

1979 • 116 citations

Phospholipids in ram semen: metabolism of plasmalogen and fatty acids

1961 • 116 citations

Evidence that Steroid Sulfates Serve as Biosynthetic Intermediates.^* IV. Conversion of Cholesterol Sulfate in vivo to Urinary C₁₉ and C₂₁ Steroidal Sulfates^†

1964 • 114 citations

Quantitation of the in vitro free cholesterol exchange of human red cells and lipoproteins

1970 • 109 citations

Inhibition of cation contransport by cholesterol enrichment of human red cell membranes

1975 • 108 citations

PLASMA LIPOPROTEINS IN FOLLICULAR FLUID OF HUMAN OVARIES

1980 • 99 citations

In vitro Fertilization of Mouse Eggs in the Presence of Bovine Follicular Fluid

1969 • 99 citations

Ca²⁺ Is Required for Mouse Sperm Capacitation and Fertilization In Vitro

1982 • 97 citations

Lysolecithin and Cholesterol Interact Stoichiometrically Forming Bimolecular Lamellar Structures in the Presence of Excess Water, or Lysolecithin or Cholesterol

1975 • 93 citations

p-Aminobenzamidine, an acrosin inhibitor, inhibits mouse sperm penetration of the zona pellucida but not the acrosome reaction

1982 • 91 citations

Studies of phospholipase A₂ related to the hamster sperm acrosome reaction

1982 • 91 citations

Cholesterol sulfate. I. Occurrence and possible biological functions as an amphipathic lipid in the membrane of the human erythrocyte

1974 • 90 citations

Capacitation of rabbit spermatozoa in the fallopian tube and in the uterus

1962 • 89 citations

Isolation and Characterization of a Novel Sulfoglycolipid, ‘ Seminolipid,’ from Boar Testis and Spermatozoa<xref ref-type="fn" rid="fn1"><sup>*</sup></xref>

1973 • 87 citations

Mechanism of induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in human leukocytes.

1977 • 86 citations

Acceleration of the Acrosome Reaction and Activation of Guinea Pig Spermatozoa by Detergents and Other Reagents

1975 • 86 citations

Interaction of Lipids with the Plasma Membrane of Sperm Cells. I. The Antifertilization Action of Cholesterol

1980 • 85 citations

An efficient method for modulation of cholesterol level in cell membranes

1978 • 83 citations

CAPACITATION OF HAMSTER SPERMATOZOA IN VITRO: THE ROLE OF CUMULUS COMPONENTS

1972 • 82 citations

Evidence for a Role of Post‐Ovulatory Cumulus Components in Supporting Fertilizing Ability of Hamster Spermatozoa

1982 • 78 citations

Cholesteryl sulfate and sterol sulfatase in the human reproductive tract

1976 • 74 citations

Biology of Mammalian Fertilization and Implantation.

1972 • 71 citations

Modulation of phospholipase A2 activity associated with human sperm membranes by divalent cations and calcium antagonists

1984 • 70 citations

Role of sperm-surface glycoproteins in gamete recognition in two mouse species

1984 • 69 citations

Mechanism of Capacitation of Rabbit Spermatozoa

1964 • 67 citations

Acceleration of acrosome reaction in hamster spermatozoa by lysolecithin

1982 • 65 citations

Removal of Sperm-Bound Seminal Plasma Components as a Prerequisite to Induction of the Rabbit Acrosome Reaction

1976 • 65 citations

CHARACTERIZATION AND ISOLATION OF A SPERM-COATING ANTIGEN FROM RABBIT SEMINAL PLASMA WITH CAPACITY TO BLOCK FERTILIZATION

1969 • 65 citations

Ionophore A23187 induces acrosome reactions in sea urchin and guinea pig spermatozoa

1976 • 64 citations

FATTY ACID COMPOSITION OF LIPIDS OF BULL, BOAR, RABBIT AND HUMAN SEMEN

1969 • 64 citations

Phospholipid Methylation Increases During Capacitation of Golden Hamster Sperm in Vitro

1983 • 62 citations

ULTRASTRUCTURAL LOCALIZATION OF SURFACE RECEPTORS FOR CONCANAVALIN A ON RABBIT SPERMATOZOA

1974 • 62 citations

Cell surface changes associated with in Vitro capacitation of hamster sperm

1978 • 62 citations

Inhibitory Effect of Synthetic Phospholipid Vesicles Containing Cholesterol on the Fertilizing Ability of Rabbit Spermatozoa

1976 • 62 citations

Structure of 1-acyl lysophosphatidylcholine and fatty acid complex in bilayers

1981 • 62 citations

A Spin Label Study of the Influence of Cholesterol on Egg Lecithin Multibilayers

1972 • 62 citations

FLUCTUATIONS IN HUMAN SERUM LIPOPROTEINS DURING THE NORMAL MENSTRUAL CYCLE

1965 • 60 citations

Phospholipase activity of sea urchin sperm: Its possible involvement in membrane fusion

1976 • 59 citations

Enzymatic and morphological events in mammalian fertilization

1978 • 59 citations

Stimulation of an exocytotic event, the hamster sperm acrosome reaction, by cis‐unsaturated fatty acids

1983 • 59 citations

Capacitation of Hamster Spermatozoa by Bovine Follicular Fluid

1969 • 55 citations

Activation of phospholipase A2 by freshly added lysophospholipids

1983 • 55 citations

Phospholipases in human seminal plasma

1974 • 54 citations

Effect of Trypsin Inhibitors on Acrosome Reaction of Guinea Pig Spermatozoa

1982 • 53 citations

FINE STRUCTURE OF THE SPERM HEAD IN EJACULATE AND UTERINE SPERMATOZOA OF THE RABBIT

1964 • 53 citations

Phospholipase A of Guinea Pig Spermatozoa: Its Preliminary Characterization and Possible Involvement in the Acrosome Reaction. (guinea pig/spermatozoa/acrosome reaction/phospholipase A)

1982 • 52 citations

Effect of cholesterol and water on the rigidity and order of phosphatidylcholine bilayers

1972 • 52 citations

Cell Polarography. II. Effect of Neuraminidase and Follicular Fluid Upon the Surface Characteristics of Human Spermatozoa

1973 • 52 citations

Cholesterol distribution between the two halves of the lipid bilayer of human erythrocyte ghost membranes.

1978 • 51 citations

DECREASE IN THE ELECTROPHORETIC MOBILITY OF RABBIT SPERMATOZOA FOLLOWING INTRA-UTERINE INCUBATION

1971 • 49 citations

Freeze-fracture alterations in guinea pig sperm membranes preceding gamete fusion.

1980 • 49 citations

Uterine fluid proteins bind sperm cholesterol during capacitation in the rabbit

1982 • 48 citations

Synthesis and turnover of sulfogalactoglycerolipid, a membrane lipid, during spermatogenesis

1979 • 47 citations

Capacitation of spermatozoa.

1967 • 47 citations

Metabolism of lipid and carbohydrate in sea urchin spermatozoa

1983 • 46 citations

The Sterol and Sterol Sulfate Content of the Male Hamster Reproductive Tract

1979 • 45 citations

Red cell surface structure: Stabilization by cholesterol sulfate as evidenced by scanning electron microscopy

1975 • 44 citations

Effects of ethynyl estradiol on serum lipoprotein lipids in male and female rats

1978 • 44 citations

CAPACITATION OF HAMSTER SPERMATOZOA WITH BLOOD SERA

1970 • 43 citations

DNA synthesis in rabbit spermatozoa after treatment with lysolecithin and fusion with somatic cells

1972 • 43 citations

Evidence suggesting the importance of fatty acids and the fatty acid moieties of sperm membrane phospholipids in the acrosome reaction of guinea pig spermatozoa

1984 • 43 citations

Phospholipase a and lysophospholipase activities in isolated fat cells: Effect of cyclic 3'.5'-AMP

1972 • 40 citations

A Study of Phospholipase in Albumin and its Role in Inducing the Acrosome Reaction of Guinea Pig Spermatozoa in Vitro

1983 • 40 citations

Ultrastructural Observations on the Penetration of Human Sperm into the Zona Pellucida of the Human Egg In Vitro

1982 • 40 citations

Morphological aspects of sperm capacitation in mammals.

1970 • 39 citations

Report of a Workshop. Maturation of the Fertilizing Ability of Mammalian Spermatozoa in the Male and Female Reproductive Tract12

1974 • 38 citations

The inhibition of acrosin by sterol sulphates

1980 • 36 citations

Destabilization of Human Sperm Membranes by Albumin, EDTA and Histidine

1978 • 36 citations

Cytochemical studies of uterine and trophoblastic surface coats during blastocyst attachment in the ewe

1982 • 35 citations

Kinetics of hydrolysis of dispersions of saturated phosphatidylcholines by Crotalus atrox phospholipase A₂

1978 • 33 citations

The Effect of Inhibitors of Trypsin and Phospholipase A₂ on the Penetration of Zona Pellucida‐free Hamster Eggs by Acrosome‐reacted Hamster Sperm

1982 • 33 citations

DESMOSTERYL SULPHATE OF HAMSTER SPERMATOZOA, A POTENT INHIBITOR OF CAPACITATION IN VITRO

1975 • 33 citations

Biosynthesis of lipids by human and fish spermatozoa

1966 • 33 citations

EFFECT OF HUMAN FOLLICULAR AND TUBAL FLUIDS ON HUMAN, MOUSE AND RAT SPERMATOZOA IN VITRO

1972 • 30 citations

FERTILIZATION OF BOVINE FOLLICULAR OOCYTES IN BOVINE AND PORCINE OVIDUCTS

1975 • 29 citations

Calcium binding to the acrosomal membrane of human spermatozoa

1975 • 29 citations

Lecithin: Cholesterol acyl transferase (LCAT)

1982 • 29 citations

CAPACITATION OF MOUSE SPERMATOZOA IN VITRO: INVOLVEMENT OF EPIDIDYMAL SECRETIONS AND CUMULUS OOPHORUS

1974 • 28 citations

Steroid Sulfatase Activity of the Hamster Reproductive Tract During the Estrous Cycle

1980 • 27 citations

Biochemistry of sperm capacitation

1983 • 26 citations

THE PHYSICAL PROPERTIES OF LYSOLECITHIN AND ITS SOLS: Part II. Refractive Indices and Densities of Sols. Micelle Formation

1958 • 26 citations

Fertilization of Squirrel Monkey and Hamster Ova in the Rabbit Oviduct (Xenogenous Fertilization)

1980 • 26 citations

Distribution of Steroid Sulfotransferase in the Male Hamster Reproductive Tract

1984 • 26 citations

The binding of sterol sulfates to hamster spermatozoa

1979 • 25 citations

Cholesteryl sulfate‐phosphatidylcholine interactions

1984 • 25 citations

Determination of critical micelle concentration of aqueous lysolecithin solutions

1971 • 25 citations

Studies on Neuraminidase Activity of the Rabbit Endometrium

1980 • 25 citations

A RE-EXAMINATION OF THE ROLE OF PHOSPHOLIPIDS AS ENERGY SUBSTRATES DURING INCUBATION OF RAM SPERMATOZOA

1973 • 24 citations

THE FERTILIZING CAPACITY OF SPERMATOZOA

1955 • 24 citations

The exchangeability of human erythrocyte membrane cholesterol

1978 • 23 citations

N^α-Benzoyl-L-arginine Ethyl Ester Splitting Activity (Acrosin) in Human Spermatozoa and Seminal Plasma during Aging in Vitro

1975 • 23 citations

Absence of species specificity for mammalian sperm capacitation in vivo

1981 • 23 citations

Studies on the mechanism of capacitation: Changes in plasma membrane proteins of rat spermatozoa during incubation invitro

1979 • 21 citations

The target sizes of the in situ and solubilized forms of human placental steroid sulfatase as measured by radiation inactivation☆

1983 • 20 citations

Ultrastructure of Rabbit Spermatozoa After Treatment with Lysolecithin and in the Presence of Hamster Somatic Cells

1973 • 19 citations

Antifertility action of a sterol sulphate in the rabbit

1982 • 19 citations

Inhibition of the hamster sperm acrosome reaction by transmethylation inhibitors

1981 • 19 citations

Reversible attachment and localization of the acid-stable seminal plasma acrosin-trypsin inhibitors on boar spermatozoa as revealed by the indirect immunofluorescent staining technique.

1975 • 19 citations

Metabolism and role of sulpholipids in mammalian tissues

1978 • 19 citations

Lysolecithin–Cholenterol Interaction. A Spin-Resonance and Electron-Micrographic Study

1975 • 18 citations

Steroid hormones and the fertilizing capacity of spermatozoa

1973 • 18 citations

ρ as a quantitative measure of transition state structure

1979 • 16 citations

Sperm glycoproteins of the boar, bull, rabbit, and ram: II. Surface glycoproteins and free acidic groups

1979 • 15 citations

Some experiments pertaining to the chemical mechanisms of the cortical reaction in fertilization of sea urchin eggs

1955 • 14 citations

Capacitation and the acrosome reaction in guinea pig spermatazoa increase the availability of surface aminophospholipids for labelling by trinitrobenzene sulphonate

1983 • 14 citations

Association of [14C] Phosphatidylcholine with Rat Epididymal Sperm and Its Conversion to [14C] Glycerylphosphorylcholine by Sperm and Principal Cells

1981 • 12 citations

Phospholipids in Guinea Pig Spermatozoa Before and After Capacitation In Vitro

2009 • 11 citations

Steroid sulfotransferase in hamster epididymis

1981 • 10 citations

Accumulation, Nature, and Possible Functions of the Malachite Green Affinity Material in Ejaculated Human Spermatozoa

1979 • 10 citations

The Physical Properties of Lysolecithin and Its Sols

1959 • 7 citations

A model for studying LCAT reaction: In vitro cholesterol esterification in pig ovarian follicular fluid

1978 • 6 citations

The haemolytic activity of sols containing progesterone solubilized by phosphatidylcholine and lysophosphatidylcholine

1969 • 6 citations

The Interaction of Lysophosphatidylcholine with Protein-Containing Liposomes1

1981 • 5 citations

Sperm Capacitation

1977 • 4 citations

Transfer of cholesterol and lecithin between erythrocytes and serum in vitro.

1976 • 3 citations

Deleted Work

1955 • 0 citations