Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility.

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules.

1990 • 509 citations

Nucleotide Sequence of Potato Virus Y (N Strain) Genomic RNA

1989 • 336 citations

A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus

1990 • 296 citations

The nucleotide sequence of tobacco vein mottling virus RNA

1986 • 254 citations

Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus.

1991 • 251 citations

Tagging of plant potyvirus replication and movement by insertion of beta-glucuronidase into the viral polyprotein.

1992 • 232 citations

Site-directed mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants

1992 • 209 citations

Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene

1993 • 134 citations

A determinant of disease symptom severity is located in the 3'-terminal noncoding region of the RNA of a plant virus.

1991 • 89 citations

Infectious in vitro transcripts from cloned cDNA of a potyvirus, tobacco vein mottling virus.

1989 • 89 citations

Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C

1990 • 87 citations

Partial purification and characterization of the potato virus Y helper component

1977 • 83 citations

Small cysteine-rich proteins of different groups of plant rna viruses are related to different families of nucleic acid-binding proteins

1991 • 66 citations

In Vitro cleavage at or near the N-terminus of the helper component protein in the tobacco vein mottling virus polyprotein

1991 • 57 citations

Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses.

1989 • 55 citations

HTLV-I tax is a zinc-binding protein: Role of zinc in tax structure and function

1992 • 38 citations

Application of genome sequence information in potyvirus taxonomy: an overview

1992 • 24 citations

Construction of in-frame chimeric plant viral genes by simplified PCR strategies

1992 • 4 citations