Freeze substitution after fast‐freeze fixation in preparation for immunocytochemistry

Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.

1979 • 1,408 citations

Immunochemistry on ultrathin frozen sections

1980 • 755 citations

Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

1981 • 721 citations

Resin development for electron microscopy and an analysis of embedding at low temperature*

1982 • 714 citations

Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique.

1983 • 640 citations

Application of cryoultramicrotomy to immunocytochemistry

1986 • 516 citations

On the preparation of cryosections for immunocytochemistry

1984 • 468 citations

Advances in ultrarapid freezing for the preservation of cellular ultrastructure

1986 • 432 citations

Theory and Practice of High Pressure Freezing

1987 • 430 citations

Cryotechniques in biological electron microscopy

1988 • 406 citations

Immunolabelling for electron microscopy

1983 • 335 citations

Areas of Adhesion between Wall and Membrane of Escherichia coli

1968 • 312 citations

A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope

1983 • 299 citations

Techniques in immunocytochemistry

1983 • 298 citations

Periplasmic gel: new concept resulting from the reinvestigation of bacterial cell envelope ultrastructure by new methods

1984 • 280 citations

Electron microscopy of frozen-hydrated bacteria

1983 • 244 citations

Practical Methods in ELECTRON MICROSCOPY

1977 • 232 citations

Freeze-Substitution and Freeze-Drying

1987 • 228 citations

Cartilage ultrastructure after high pressure freezing, freeze substitution, and low temperature embedding. I. Chondrocyte ultrastructure--implications for the theories of mineralization and vascular invasion.

1984 • 225 citations

The organization of myosin and actin in rapid frozen nerve growth cones.

1989 • 215 citations

Freezing in a propane jet and its application in freeze-fracturing.

1980 • 212 citations

New instruments which facilitate rapid freezing at 83 K and 6 K

1982 • 211 citations

Colloidal Gold Probes in Immunocytochemistry

1983 • 206 citations

Cryofixation: A Tool In Biological Ultrastructural Research

1982 • 198 citations

Shape and fine structure of nucleoids observed on sections of ultrarapidly frozen and cryosubstituted bacteria

1985 • 185 citations

Immunogold labeling in cell biology

1992 • 184 citations

Modern acrylics for post-embedding immunostaining techniques.

1987 • 171 citations

Low temperature embedding with Lowicryl resins: two new formulations and some applications

1985 • 170 citations

Improved Cryofixation Applicable to Freeze Etching

1971 • 162 citations

A method for rapid freeze fixation of plant cells

1986 • 151 citations

Immunolabelling for electron microscopy

1986 • 140 citations

Cartilage ultrastructure after high pressure freezing, freeze substitution, and low temperature embedding. II. Intercellular matrix ultrastructure - preservation of proteoglycans in their native state.

1984 • 138 citations

The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance.

1984 • 126 citations

Freeze-substitution and Lowicryl HM20 embedding of fixed rat brain: suitability for immunogold ultrastructural localization of neural antigens.

1991 • 124 citations

Intracellular location of the histonelike protein HU in Escherichia coli

1988 • 124 citations

Demonstration of intra- and extracellular localization of bullous pemphigoid antigen using cryofixation and freeze substitution for postembedding immunoelectron microscopy

1989 • 116 citations

Freeze‐substitution

1982 • 114 citations

Evaluation of freeze-substitution and conventional embedding protocols for routine electron microscopic processing of eubacteria

1990 • 111 citations

Rapid freezing and electron microscopy for the arrest of physiological processes

1974 • 110 citations

Cryofixation Without Pretreatment at Ambient Pressure

1987 • 108 citations

Microprobe Analysis of Biological Systems

1981 • 104 citations

Liquid nitrogen‐based quick freezing: Experiences with bounce‐free delivery of cholinergic nerve terminals to a metal surface

1984 • 103 citations

Freeze Substitution and Low-Temperature Embedding

1992 • 98 citations

A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution

1986 • 93 citations

Characterization of the bioluminescent organelles in Gonyaulax polyedra (dinoflagellates) after fast-freeze fixation and antiluciferase immunogold staining.

1987 • 93 citations

Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii‐cells for electron microscopy

1983 • 90 citations

Developments of new Lowicryl® resins for embedding biological specimens at even lower temperatures

1986 • 87 citations

Adult rat cardiomyocytes cultured in creatine-deficient medium display large mitochondria with paracrystalline inclusions, enriched for creatine kinase.

1991 • 81 citations

Extraction of proteins and membrane lipids during low temperature embedding of biological material for electron microscopy

1986 • 81 citations

Immunogold labelling of actin on sections of freeze-substituted plant cells

1989 • 80 citations

THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITY

1982 • 80 citations

Immunoelectron microscopic exploration of the Golgi complex.

1983 • 79 citations

Immunogold electron microscopy of phytochrome in Avena: identification of intracellular sites responsible for phytochrome sequestering and enhanced pelletability.

1986 • 77 citations

Extraction of lipids during freeze‐substitution of Acholeplasma laidlawii‐cells for electron microscopy

1984 • 76 citations

Freeze‐substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry

1990 • 58 citations

Advantages of fast‐freeze fixation followed by freeze‐substitution for the preservation of cell integrity

1991 • 57 citations

Immunoelectron microscopy of tissues processed by rapid freezing and freeze-substitution fixation without chemical fixatives: application to catalase in rat liver hepatocytes.

1990 • 56 citations

On the physical identity of scintillons: Bioluminescent particles in Gonyaulax polyedra

1972 • 56 citations

Use of on-section immunolabeling and cryosubstitution for studies of bacterial DNA distribution

1987 • 56 citations

Ultrastructural immunocytochemical localization of LH and FSH in the pituitary of the untreated male rat

1984 • 53 citations

Effect of chemical fixatives on accurate preservation of Escherichia coli and Bacillus subtilis structure in cells prepared by freeze-substitution

1990 • 52 citations

Cryofixation of Dynamic Processes in Cells and Organelles

1987 • 51 citations

In situ localization of cartilage extracellular matrix components by immunoelectron microscopy after cryotechnical tissue processing.

1987 • 49 citations

A gentle, bounce-free assembly for quick-freezing tissues for electron microscopy: Application to isolated torpedine ray electrocyte stacks

1979 • 48 citations

Low Temperature Embedding

1989 • 44 citations

Electron-microscopic demonstration of olfactory-marker protein with protein G-gold in freeze-substituted, Lowicryl K11M-embedded rat olfactory-receptor cells

1989 • 43 citations

Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity.

1991 • 40 citations

Experiments on freezing damage with freeze substitution using moth antennae as test objects

1982 • 40 citations

Immunogold labeling of luciferase in the luminous bacterium Vibrio harveyi after fast-freeze fixation and different freeze-substitution and embedding procedures.

1989 • 39 citations

Ultrastructural analysis ofErysiphe graminis haustoria and subcuticular stroma ofVenturia inaequalis using cryosubstitution

1986 • 36 citations

An excursion through the ultrastructural world of quick‐frozen pancreatic islets

1986 • 36 citations

Immunocytochemical localization of amylase in gerbil salivary gland acinar cells processed by rapid freezing and freeze-substitution fixation.

1989 • 34 citations

‘Lipidic particle’ systems as visualized by thin-section electron microscopy

1985 • 34 citations

Immunoelectron microscopic study of a new D-amino acid oxidase-immunoreactive subcompartment in rat liver peroxisomes.

1991 • 34 citations

An Improvement of the Device for Rapid Freezing by Use of Liquid Propane and the Application of Immunocytochemistry to the Resin Section of Rapid-Frozen, Substitution Fixed Anterior Pituitary Gland

1982 • 31 citations

Contribution of new cryomethods to a better knowledge of bacterial anatomy

1988 • 29 citations

Colocalization of luciferin binding protein and luciferase to the scintillons ofGonyaulax polyedra revealed by double immunolabeling after fast-freeze fixation

1991 • 29 citations

Lowicryl resins in microbiology

1990 • 27 citations

Cryoelectron Microscopy of Vitrified Specimens

1987 • 27 citations

Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach

1985 • 26 citations

An optional dyadic junctional complex revealed by fast-freeze fixation in the bioluminescent system of the scale worm.

1987 • 23 citations

Immunolabeling of adenohypophysial cells with protein A-colloidal gold--antibody complex for electron microscopy: use of the freeze-substitution technique in tissue preparation.

1984 • 23 citations

Optimal preparatory procedures of cryofixation for immunocytochemistry

1989 • 22 citations

Theoretical analysis of specimen cooling rate during impact freezing and liquid-jet freezing of freeze-etch specimens

1982 • 22 citations

New Electron Microscopic Data on the Structure of the Nucleoid and Their Functional Consequences

1986 • 22 citations

Cryofixation and ultra‐low‐temperature freeze‐drying as a preparative technique for TEM

1991 • 21 citations

Immunoelectron microscopy of acetylcholine receptors and 43 KD protein after rapid freezing, freeze-substitution, and low-temperature embedding in lowicryl K11M.

1991 • 19 citations

Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.

1989 • 18 citations

A new organelle related to osmoregulation in ultrarapidly frozenPelvetia embryos

1989 • 18 citations

New aspects of bacterial ultrastructure as revealed by modern acrylics for electron microscopy

1990 • 17 citations

Common sense in electron microscopy About cryofixation, freeze-substitution, low temperature embedding, and low denaturation embedding

1990 • 17 citations

The Effects of Freeze-Substitution Media on the Ultrastructure of Inclusion Bodies in Type II Pneumocytes of Mouse Lung Processed by the Cryofixation Method

1984 • 16 citations

QUICK FREEZING AND FREEZE SUBSTITUTION FOR X-RAY MICROANALYSIS OF CALCIUM

1981 • 16 citations

Cryofixation

1989 • 15 citations

Electron microscopic and immunocytochemical study of rapidly frozen, freeze-substituted neural lobes of rats

1991 • 15 citations

Ultrastructural localization of the histone-like protein HTa from the archaeon Thermoplasma acidophilum

1990 • 12 citations

An application of cryofixation to immunocytochemistry.

1987 • 12 citations

Expression and localization of bacterial luciferase determined by immunogold labeling

1989 • 11 citations

The histochemistry of asparagine-linked oligosaccharides of glycoproteins in mammalian and avian tissues as studied by digestion with almond glycopeptidase

1985 • 4 citations