Both the 25-kDa and 50-kDa domains in myosin subfragment 1 are close to the reactive thiols.
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Abstract
References (28)
Studies on the chymotryptic digestion of myosin. Effects of divalent cations on proteolytic susceptibility
1977 • 632 citations
The characterization of myosin–product complexes and of product-release steps during the magnesium ion-dependent adenosine triphosphatase reaction
1974 • 364 citations
Structure of the actin–myosin interface
1981 • 349 citations
Fluorescence studies on heavy meromyosin-substrate interaction
1972 • 222 citations
Location of SH-1 and SH-2 in the heavy chain segment of heavy meromyosin
1978 • 186 citations
Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment 1.
1979 • 139 citations
Interaction of Heavy Meromyosin with Substrate
1967 • 137 citations
Sulfhydryl Groups Involved in the Active Site of Myosin A Adenosine Triphosphatase*
1966 • 135 citations
Cooperative role of two sulfhydryl groups in myosin adenosine triphosphatase
1974 • 120 citations
The substructure of heavy meromyosin. The effect of Ca2+ and Mg2+ on the tryptic fragmentation of heavy meromyosin.
1975 • 119 citations
Photoaffinity labelling with an ATP analog of the N-terminal peptide of myosin
1979 • 117 citations
Molecular movements promoted by metal nucleotides in the heavy-chain regions of myosin heads from skeletal muscle
1985 • 102 citations
Effect of nucleotide binding on the proximity of the essential sulfhydryl groups of myosin. Chemical probing of movement of residues during conformational transitions
1977 • 99 citations
Identification of an active site peptide of skeletal myosin after photoaffinity labeling with N-(4-azido-2-nitrophenyl)-2-aminoethyl diphosphate.
1985 • 99 citations
Effect of nucleotides and pyrophosphate on spin labels bound to S1 thiol groups of myosin
1970 • 92 citations
Photochemical probes of the active site of myosin. Irradiation of trapped 3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate labels the 50-kilodalton heavy chain tryptic peptide.
1984 • 78 citations
The conformation of myosin during the steady state of ATP hydrolysis: Studies with myosin spin labeled at the S1 thiol groups
1971 • 77 citations
Electron microscopic visualization of the SH1 thiol of myosin by the use of an avidin-biotin system
1984 • 74 citations
Cross-linking of myosin subfragment 1. Nucleotide-enhanced modification by a variety of bifunctional reagents.
1980 • 57 citations
Excitation energy transfer studies on the proximity between SH1 and the adenosinetriphosphatase site in myosin subfragment 1
1981 • 43 citations
Substructure of Myosin Subfragment-1 as Revealed by Digestion with Proteolytic Enzymes
1980 • 41 citations
Circular dichroism of the adenine and 6-mercaptopurine nucleotide complexes of heavy meromyosin
1974 • 40 citations
Effect of nucleotides, divalent cations and temperature on the tryptic susceptibility of myosin subfragment 1
1984 • 38 citations
Tryptic digestion as a probe of myosin S-1 conformation.
1982 • 37 citations
Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.
1985 • 36 citations
Crosslinking of actin to myosin subfragment 1 in the presence of nucleotides
1985 • 25 citations
Introduction of a donor-acceptor pair by a single protein modification. Förster energy transfer distance measurements from trapped 1,N6-ethenoadenosine diphosphate to chromophoric cross-linking reagents on the critical thiols of myosin subfragment.
1984 • 21 citations
Photosensitized Direct Cross-Linking of Fluorescent Analogs of ATP to the Adenine Recognition Domain in Myosin ATPase
1985 • 19 citations