Expression of recombinant soluble and membrane‐bound catechol O‐methyltransferase in eukaryotic cells and identification of the respective enzymes in rat brain

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

1987 • 4,937 citations

Solubilization of membranes by detergents

1975 • 2,909 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Enzymatic O-Methylation of Epinephrine and Other Catechols

1958 • 1,291 citations

Baculovirus Expression Vectors: the Requirements for High Level Expression of Proteins, Including Glycoproteins

1987 • 677 citations

[16] Isolation of subcellular organelles

1990 • 563 citations

Catechol-O-methyl transferase: pharmacological aspects and physiological role.

1975 • 547 citations

Chromosomal mapping of the human catechol-O-methyltransferase gene to 22q11.1→q11.2

1992 • 295 citations

Human catechol-O-methyltransferase: cloning and expression of the membrane-associated form.

1991 • 197 citations

Cloning and Characterization of Human Placental Catechol--Methyltransferase cDNA

1991 • 166 citations

Design of enzyme inhibitors as drugs

1990 • 154 citations

Rationale for Selective COMT Inhibitors as Adjuncts in the Drug Treatment of Parkinson's Disease

1990 • 137 citations

Molecular cloning and characterization of rat liver catechol-O-methyltransferase

1990 • 118 citations

Distinct Cellular Localization of Membrane‐Bound and Soluble Forms of Catechol‐O‐Methyltransferase in Brain

1983 • 111 citations

Catechol O-methyltransferase. IV. Factors affecting m- and p-methylation of substituted catechols.

1972 • 100 citations

Characterization of Membrane‐Bound and Soluble Catechol‐O‐Methyltransferase from Human Frontal Cortex

1984 • 95 citations

The human catechol-O-methyltransferase (COMT) gene maps to band q11.2 of chromosome 22 and shows a frequent RFLP with <i>Bgl</i>I

1992 • 81 citations

Catechol O-methyltransferase. 3. M- and p-O-methylation of catecholamines and their metabolites.

1970 • 80 citations

Soluble and Particulate Forms of Rat Catechol-O-Methyltransferase Distinguished by Gel Electrophoresis and Immune Fixation

1985 • 70 citations

Striatal membrane-bound and soluble catechol-O-methyl-transferase after selective neuronal lesions in the rat

1987 • 63 citations

Rapid isolation of neuroblastoma plasma membranes on Percoll gradients. Characterization and lipid composition

1985 • 55 citations

Multiple molecular forms of catechol-O-methyltransferase. Evidence for two distinct forms, and their purification and physical characterization.

1979 • 53 citations

Determination of catechol‐O‐methyltransferase activity in erythrocytes by high performance liquid chromatography with electrochemical detection

1989 • 50 citations

Design of enzyme inhibitors as drugs

1989 • 48 citations

Solubilization and partial purification of particulate catechol-O-methyltransferase from rat liver

1977 • 44 citations

Cell‐free synthesis of rat and human catechol O‐methyltransferase

1991 • 41 citations

Expression of enzymatically active rat liver and human placental catechol-O-methyltransferase in Escherichia coli; purification and partial characterization of the enzyme

1992 • 40 citations

Purification and partial characterization of rat liver soluble catechol‐ O‐methyltransferase

1990 • 40 citations

A rapid isolation procedure of plasma membranes from human neutrophils using self-generating Percoll gradients. Importance of pH in avoiding contamination by intracellular membranes

1985 • 38 citations

Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta: Comparison to the rat liver enzyme

1991 • 28 citations

Immunoaffinity purification and partial amino acid sequence analysis of catechol-O-methyltransferase from pig liver

1991 • 26 citations

Role of brain lysosomes in the development of manganese toxicity in mice

1983 • 26 citations

Crystallization and preliminary X-ray investigation of a recombinant form of rat catecholO-methyltransferase

1991 • 25 citations

Inhibition of human erythrocyte and gastroduodenal catechol-O-methyltransferase activity by nitecapone

1991 • 24 citations

The site of O-methylation by membrane-bound catechol-O-methyltransferase

1984 • 22 citations

Catechol-O-methyl transferase in mouse liver plasma membranes

1975 • 22 citations

An EBV-based mammalian cell expression vector for efficient expression of cloned coding sequences

1988 • 21 citations

Two sensitive, convenient, and widely applicable assays for marker enzyme activities specific to endoplasmic reticulum

1988 • 15 citations

The cellular location of catechol-O-methyltransferase in rat liver

1982 • 14 citations

Efficient synthesis of influenza virus hemagglutinin in mammalian cells with an extrachromosomal Epstein-Barr virus vector

1989 • 10 citations

Comparison of mammalian cell expression vectors with and without an EBV-replicon

1988 • 10 citations

Production of human tissue-type plasminogen activator in different mammalian cell lines using an Epstein-Barr virus vector

1990 • 9 citations

Monoamine Methyltransferases: Phenylethanolamine N-Methyltransferase and Catechol O-Methyltransferase

2003 • 5 citations