A highly sensitive cytotoxicity assay based on the release of reporter enzymes, from stably transfected cell lines

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

1992 • 4,885 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.

1982 • 1,371 citations

Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.

1968 • 1,363 citations

An enzyme-release assay for natural cytotoxicity

1983 • 1,063 citations

Luciferase reporter gene vectors for analysis of promoters and enhancers.

1988 • 1,016 citations

The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity

1993 • 886 citations

Electroporation for the efficient transfection of mammalian cells with DNA

1987 • 856 citations

Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.

1994 • 777 citations

A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

1987 • 767 citations

A new dominant hybrid selective marker for higher eukaryotic cells

1981 • 662 citations

Bioluminescence and chemiluminescence. Current status

1991 • 296 citations

CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity

1994 • 267 citations

A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: Application to quantitation of β-galactosidase in lacZ-transfected cells

1991 • 223 citations

Hybrid cell lines with T-cell characteristics

1977 • 146 citations

Optimization of the tetrazolium-based colorimetric assay for the measurement of cell number and cytotoxicity

1988 • 124 citations

Measurement of lymphocyte cytotoxicity by assessing endogenous alkaline phosphatase activity of the target cells

1981 • 72 citations

Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells

1988 • 58 citations

Cellular immune response against tumor cells

1973 • 53 citations

Differentiation of cytotoxicity using target cells labelled with europium and samarium by electroporation

1994 • 20 citations

Simultaneous measurement of natural killer cell cytotoxicity against each of three different target cell lines

1994 • 13 citations