Secretion of glycosylated human erythropoietin from yeast directed by the α-factor leader region

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Correction of the Anemia of End-Stage Renal Disease with Recombinant Human Erythropoietin

1987 • 2,006 citations

EFFECT OF HUMAN ERYTHROPOIETIN DERIVED FROM RECOMBINANT DNA ON THE ANAEMIA OF PATIENTS MAINTAINED BY CHRONIC HAEMODIALYSIS

1986 • 1,412 citations

Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes

1986 • 1,148 citations

Cloning and expression of the human erythropoietin gene.

1985 • 1,144 citations

Isolation and characterization of genomic and cDNA clones of human erythropoietin

1985 • 1,101 citations

Isolation of a yeast centromere and construction of functional small circular chromosomes

1980 • 788 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

1982 • 575 citations

The Release of Intact Oligosaccharides from Specific Glycoproteins by Endo-β-N-acetylglucosaminidase H

1974 • 499 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Structural characterization of human erythropoietin.

1986 • 375 citations

Structure and Biosynthesis of the Mannan Component of the Yeast Cell Envelope

1976 • 333 citations

Heterologous Protein Secretion from Yeast

1985 • 299 citations

Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae

1984 • 190 citations

Chemical and enzymatic synthesis of gene fragments

1983 • 174 citations

Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter

1984 • 174 citations

Protein secretion from Saccharomyces cerevisiae directed by the prepro-alpha-factor leader region.

1986 • 144 citations

Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.

1984 • 126 citations

Erythropoietin: Gene Cloning, Protein Structure, and Biological Properties

1986 • 110 citations

An Assay for Erythropoietin in Vitro at the Milliunit Level

1975 • 94 citations

Isolation and characterization of the structural gene for secreted acid phosphatase from Schizosaccharomyces pombe.

1986 • 91 citations

Synthesis, secretion and processing of α-factor-interferon fusion proteins Id yeast

1984 • 81 citations

Controlled expression and purification of human immune interferon from high‐cell‐density fermentations of Saccharomyces cerevisiae

1987 • 74 citations

Structural Analysis and Sequence Organization of Yeast Centromeres

1983 • 70 citations

Improved Secretion of Heterologous Proteins bySaccharomyces cerevisiae: Effects of Promoter Substitution in Alpha-Factor Fusions

1986 • 58 citations

[33] Expression and secretion vectors for yeast

1987 • 57 citations

Secretion of Heterologous Proteins from Saccharomyces Cerevisiae

1987 • 46 citations

Expression of interferon-gamma from hybrid yeast GPD promoters containing upstream regulatory sequences from the GAL1-GAL10 intergenic region

1988 • 34 citations

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