Evidence from two-protonic-state reactivity probe kinetics that chymopapain in fresh nonfruit latex ofCarica papaya consists of multiple forms of chymopapain A. The value of catalytic site characteristics in the identification, classification, and characterization of the papaya cysteine proteinases papain, the chymopapains, and papaya proteinase Ω

Kinetics and Mechanism

1961 • 2,376 citations

ON PROTEOLYTIC ENZYMES

1936 • 57 citations

[24] Two-protonic-state electrophiles as probes of enzyme mechanism

1982 • 52 citations

Chymopapain. Chromatographic purification and immunological characterization

1984 • 47 citations

Chymopapain

1967 • 35 citations

Isolation of highly active papaya peptidases A and B from commercial chymopapain

1981 • 30 citations

A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification

1983 • 26 citations

Isolation and characterization of the four major cysteine-proteinase components of the latex of carica papaya L. reactivity characteristics towards 2,2′-dipyridyl disulfide of the thiol groups of papain, chymopapains A and B, and papaya peptidase A

1982 • 21 citations

N-terminal homology in three cysteinyl proteases from Papaya Latex

1979 • 21 citations

Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5

1983 • 15 citations

The three states criterion of nucleophilic reactivity in the conjugate acid of an anionic nucleophile and some problems of proton partitioning: implications for the reactions of 2,2′-dipyridyl disulphide with papain and with L-ergothioneine

1974 • 14 citations

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (βnuc.) for the reactions of thiolate anions with 2,2′-dipyridyl disulphide may be decreased by reagent protonation

1980 • 13 citations