Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

In vitro insertional mutagenesis with a selectable DNA fragment

1984 • 1,708 citations

Cloning and analysis of the promoter region of the erythromycin-resistance gene (ermE) of Streptomyces erythraeus

1986 • 347 citations

Intergeneric conjugation between Escherichia coli and Streptomyces species

1989 • 311 citations

Saccharopolyspora spinosa sp. nov. Isolated from Soil Collected in a Sugar Mill Rum Still

1990 • 309 citations

Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.

1983 • 285 citations

Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs.

1990 • 270 citations

A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes

1989 • 223 citations

xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter

1989 • 208 citations

Analysis of the integration function of the streptomycete bacteriophage φC31

1991 • 202 citations

Genetic Recombination in Streptomyces fradiae by Protoplast Fusion and Cell Regeneration

1978 • 153 citations

Genetics of Streptomyces Fradiae and Tylosin Biosynthesis

1988 • 136 citations

Cloning and Expression in Streptomyces lividans of Clustered Erythromycin Biosynthesis Genes from Streptomyces erythreus

1986 • 127 citations

The minimal replicon of a Streptomycete plasmid produces an ultrahigh level of plasmid DNA

1986 • 127 citations

Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.

1983 • 123 citations

Construction of a series of pSAM2-based integrative vectors for use in actinomycetes

1990 • 123 citations

[10] Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA

1987 • 98 citations

A new shuttle cosmid vector, pKC505, for streptomycetes: its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambofacienslibrary

1987 • 94 citations

Plasmid cloning vectors that integrate site-specifically in Streptomyces spp.

1991 • 91 citations

Cloning genes for the biosynthesis of a macrolide antibiotic.

1987 • 87 citations

Cloning high molecular weight DNA fragments by the bacteriophage P1 system

1992 • 78 citations

Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts

1985 • 73 citations

Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus

1991 • 63 citations

Cloning of spiramycin biosynthetic genes and their use in constructing Streptomyces ambofaciens mutants defective in spiramycin biosynthesis

1990 • 53 citations

Highly transformable mutants of Streptomyces fradiae defective in several restriction systems

1987 • 50 citations

The level of a transcript required for production of a Streptomyces coelicolor antibiotic is conditionally dependent on a tRNA gene

1990 • 47 citations

Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2

1989 • 47 citations

Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581

1984 • 41 citations

Biologically active secondary metabolites from Streptomyces

1988 • 41 citations

Phage Vectors that Allow Monitoring of Transcription of Secondary Metabolism Genes in Streptomyces

1991 • 38 citations

Antibiotics: opportunities for genetic manipulation

1989 • 29 citations

A thiostrepton-inducible expression vector for use in Streptomyces spp.

1991 • 26 citations

Site-directed transcription initiation with a mobile promoter

1989 • 6 citations

Cloning a replication function from the streptomycete bacteriophage FP43

1992 • 1 citations