The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A plant DNA minipreparation: Version II

1983 • 7,510 citations

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

A Simple and General Method for Transferring Genes into Plants

1985 • 4,589 citations

A complementation analysis of the restriction and modification of DNA in Escherichia coli

1969 • 3,866 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

1979 • 3,210 citations

Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

1980 • 2,669 citations

BinaryAgrobacteriumvectors for plant transformation

1984 • 2,178 citations

A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid

1983 • 1,758 citations

Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter

1985 • 1,387 citations

Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens

1985 • 1,011 citations

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5

1982 • 936 citations

Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants

1982 • 857 citations

A small cosmid for efficient cloning of large DNA fragments

1980 • 829 citations

Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules

1978 • 752 citations

Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability

1974 • 736 citations

Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plants

1985 • 712 citations

Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

1983 • 581 citations

Chimeric genes as dominant selectable markers in plant cells

1983 • 487 citations

The functional organization of the nopaline A. tumefaciens plasmid pTiC58

1980 • 447 citations

A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.

1985 • 421 citations

New cloning vehicles for transformation of higher plants

1985 • 392 citations

Expression of foreign genes in regenerated plants and in their progeny

1984 • 389 citations

Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955

1983 • 388 citations

The T‐region of Ti plasmids codes for an enzyme synthesizing indole‐3‐acetic acid

1984 • 367 citations

Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens.

1983 • 339 citations

A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities

1978 • 333 citations

Genetic Identification of functions of TL-DNA transcripts in octopine crown galls

1982 • 294 citations

Right 25 by terminus sequence of the nopaline t-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome

1984 • 288 citations

In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens

1979 • 261 citations

Genetic analysis of T-DNA transcripts in nopaline crown galls

1983 • 236 citations

Phaseolin Gene from Bean Is Expressed After Transfer to Sunflower Via Tumor-Inducing Plasmid Vectors

1983 • 234 citations

The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts

1985 • 233 citations

Plasmid pKC7: A vector containing ten restriction endonuclease sites suitable for cloning DNA segments

1979 • 230 citations

Purification of genomic sequences from bacteriophage libraries by recombination and selectionin vivo

1983 • 221 citations

Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria

1985 • 213 citations

The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts

1982 • 208 citations

Mini–Ti: A New Vector Strategy for Plant Genetic Engineering

1983 • 181 citations

Introduction of Genetic Material into Plant Cells

1983 • 180 citations

Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid

1983 • 168 citations

Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; Common transcripts in octopine and nopaline tumors

1983 • 163 citations

Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens

1981 • 161 citations

The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5.

1984 • 150 citations

Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens

1981 • 149 citations

Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5′-flanking sequence of a pea chlorophyll a/b -binding protein gene

1985 • 136 citations

Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco

1985 • 128 citations

Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells

1986 • 123 citations

Vectors for Transformation of Higher Plants

1985 • 120 citations

Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells.

1981 • 116 citations

Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells

1985 • 114 citations

Genes Involved in Microbe-Plant Interactions

1984 • 113 citations

Cell fusion : gene transfer and transformation

1984 • 113 citations

Complementation analysis of replication and maintenance functions of broad host range plasmids RK2 and RP1

1981 • 91 citations

Plasmid ColE1 conjugal mobility: The nature of bom, a region required in cis for transfer

1982 • 84 citations

Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria

1983 • 83 citations

A simple method to transfer, integrate and study expression of foreign genes, such as chicken ovalbumin and alpha-actin in plant tumors.

1984 • 82 citations

Simple binary vectors for DNA transfer to plant cells

1985 • 77 citations

Organ-specific expression of three leaf/stem specific cDNAs from potato is regulated by light and correlated with chloroplast development

1985 • 77 citations

Broad-host-range cloning vectors derived from the W-plasmid Sa

1982 • 76 citations

trans-Acting virulence functions of the octopine Ti plasmid from Agrobacterium tumefaciens

1984 • 72 citations

Delivery of T-DNA from the Agrobacterium tumefaciens chromosome into plant cells

1984 • 66 citations

Construction of a heat-inducible gene for plants. Demonstration of heat-inducible activity of the Drosophila hsp70 promoter in plants

1985 • 66 citations

The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.

1983 • 66 citations

T-DNA-encoded auxin formation in crown-gall cells

1985 • 65 citations

Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium

1985 • 59 citations

Isolation of a recombination deficient Agrobacterium tumefaciens mutant

1979 • 58 citations

IS-like element IS8 in RP4 plasmid and its involvement in cointegration

1980 • 49 citations

Molecular biology of plant tumours

1983 • 29 citations

Lysopine dehydrogenase activity as an early marker in crown gall transformation

1982 • 17 citations

Instability of a high-copy-number mutant of a miniplasmid derived from broad host range IncP plasmid RK2

1983 • 16 citations

Transformation of Tobacco Cells by Coculture with Agrobacterium tumefaciens

1984 • 5 citations

Transfer of foreign genes to plants and its use to study developmental processes

1984 • 4 citations