Factors affecting heterologous gene expression in Saccharomyces cerevisiae

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Experiments in molecular genetics

1972 • 28,260 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

Transformation of yeast.

1978 • 2,267 citations

Codon selection in yeast.

1982 • 1,580 citations

Trans-complementable copy-number mutants of plasmid ColE1

1980 • 975 citations

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

1982 • 854 citations

Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons in protein genes

1982 • 604 citations

Chapter 3 Isolation of Yeast DNA

1975 • 520 citations

The structure of eight distinct cloned human leukocyte interferon cDNAs

1981 • 509 citations

Conservation of high efficiency promoter sequences inSaccharomyces cerevisiae

1982 • 418 citations

Sequence and structure of yeast phosphoglycerate kinase.

1982 • 364 citations

Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae

1983 • 337 citations

Expression of a human gene for interferon in yeast

1981 • 332 citations

CHROMOSOME MAPPING IN SACCHAROMYCES: CENTROMERE-LINKED GENES

1960 • 300 citations

A method for the recovery of DNA from agarose gels

1978 • 262 citations

Expression of hepatitis B surface antigen gene in yeast.

1983 • 239 citations

Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.

1981 • 232 citations

The primary structure of theSaccharomyces cerevisiaegene for 3-phosphogrycerate kinase

1982 • 175 citations

General Method for the Detection of Cells producing Antibodies against Haptens and Proteins

1970 • 155 citations

Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase

1978 • 147 citations

Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region

1979 • 141 citations

Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

1982 • 139 citations

Characterization of two types of yeast ribosomal DNA genes

1978 • 119 citations

Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.

1983 • 115 citations

Overlap hybridization screening: Isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III

1979 • 113 citations

Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

1984 • 85 citations

Expression of the human interferon–γ cDNA in yeast

1983 • 65 citations

Expression inSaccharomyces cerevisiaeof human interferon-alpha directed by theTRP1 5′region

1983 • 54 citations

Expression of hepatitis B surface antigen in yeast.

1983 • 53 citations

The identification and high level expression of a protein encoded by the yeast Ty element.

1984 • 44 citations

Synthesis of a human insulin gene VI. expression of the synthetic proinsulin gene in yeast

1983 • 43 citations

High-level expression of an interferon alpha 2 gene cloned in phage M13mp7 and subsequent purification with a monoclonal antibody.

1982 • 42 citations

Interferon Induction by Viruses and Polynucleotides: A Differential Effect of Camptothecin

1975 • 35 citations

The complete amino acid sequence of yeast phosphoglycerate kinase

1983 • 29 citations

Construction and characterization of three yeast-Escherichia coli shuttle vectors designed for rapid subcloning of yeast genes on small DNA fragments

1981 • 29 citations

Expression of Ty-lacZfusions inSaccharomyces cerevisiae

1984 • 18 citations