Solvational Tuning of the Unfolding, Aggregation and Amyloidogenesis of Insulin

Principles of Fluorescence Spectroscopy

1999 • 27,045 citations

Seeding “one-dimensional crystallization” of amyloid: A pathogenic mechanism in Alzheimer's disease and scrapie?

1993 • 2,146 citations

Protein Denaturation

1968 • 2,108 citations

Protein misfolding, evolution and disease

1999 • 1,948 citations

Surface and Interfacial Aspects of Biomedical Polymers

1985 • 1,272 citations

Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavine T

1989 • 1,133 citations

Effect of Environmental Factors on the Kinetics of Insulin Fibril Formation: Elucidation of the Molecular Mechanism

2001 • 1,132 citations

The Control of Protein Stability and Association by Weak Interactions with Water: How Do Solvents Affect These Processes?

1993 • 1,076 citations

The Use of Circular Dichroism in the Investigation of Protein Structure and Function

2000 • 1,070 citations

Designing conditions for in vitro formation of amyloid protofilaments and fibrils

1999 • 1,016 citations

Conformational constraints for amyloid fibrillation: the importance of being unfolded

2004 • 1,000 citations

The alternative conformations of amyloidogenic proteins and their multi-step assembly pathways

1998 • 996 citations

Trifluoroethanol and colleagues: cosolvents come of age. Recent studies with peptides and proteins

1998 • 826 citations

The protofilament structure of insulin amyloid fibrils

2002 • 811 citations

Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism

1980 • 777 citations

Protein-solvent preferential interactions, protein hydration, and the modulation of biochemical reactions by solvent components

2002 • 664 citations

Methods to Estimate the Conformation of Proteins and Polypeptides from Circular Dichroism Data

1996 • 607 citations

Rotational Brownian Motion and Polarization of the Fluorescence of Solutions

1953 • 575 citations

A brief survey of methods for preparing protein conjugates with dyes, haptens and crosslinking reagents

1992 • 561 citations

Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces.

1991 • 507 citations

Spectrofluorimetric assessment of the surface hydrophobicity of proteins

1992 • 465 citations

Analysis of the circular dichroism spectrum of proteins using the convex constraint algorithm: A practical guide

1992 • 436 citations

Clustering of Fluorine-Substituted Alcohols as a Factor Responsible for Their Marked Effects on Proteins and Peptides

1999 • 367 citations

Kinetic Studies of Amyloid β-Protein Fibril Assembly

2002 • 338 citations

Convex constraint analysis: a natural deconvolution of circular dichroism curves of proteins

1991 • 329 citations

Emerging ideas on the molecular basis of protein and peptide aggregation

2003 • 316 citations

A General Model for Amyloid Fibril Assembly Based on Morphological Studies Using Atomic Force Microscopy

2003 • 295 citations

Osmolyte-driven contraction of a random coil protein

1998 • 283 citations

Amyloidogenic Self-Assembly of Insulin Aggregates Probed by High Resolution Atomic Force Microscopy

2004 • 265 citations

Manipulating the Amyloid-β Aggregation Pathway with Chemical Chaperones

1999 • 264 citations

Local and nonlocal interactions in globular proteins and mechanisms of alcohol denaturation

1993 • 256 citations

Group additive contributions to the alcohol-induced α-helix formation of melittin: implication for the mechanism of the alcohol effects on proteins

1998 • 245 citations

Conformational Behavior and Aggregation of α-Synuclein in Organic Solvents: Modeling the Effects of Membranes

2003 • 243 citations

Mechanism of Insulin Fibrillation

2004 • 236 citations

Partially Folded Intermediates in Insulin Fibrillation

2003 • 227 citations

Spectral Properties of Thioflavin T and Its Complexes with Amyloid Fibrils

2003 • 222 citations

Insulin at pH 2: Structural Analysis of the Conditions Promoting Insulin Fibre Formation

2002 • 214 citations

Kosmotropes and chaotropes: modelling preferential exclusion, binding and aggregate stability

2004 • 204 citations

Studies of the Structure of Insulin Fibrils by Fourier Transform Infrared (FTIR) Spectroscopy and Electron Microscopy

2001 • 186 citations

Solvation Phenomena of a Tetrapeptide in Water/Trifluoroethanol and Water/Ethanol Mixtures: A Diffusion NMR, Intermolecular NOE, and Molecular Dynamics Study

2002 • 174 citations

Aggregation of Bovine Insulin Probed by DSC/PPC Calorimetry and FTIR Spectroscopy

2003 • 174 citations

Protein folding in the cell: reshaping the folding funnel

2004 • 174 citations

Trifluoroethanol Promotes Helix Formation by Destabilizing Backbone Exposure: Desolvation Rather than Native Hydrogen Bonding Defines the Kinetic Pathway of Dimeric Coiled Coil Folding

1998 • 163 citations

ISOLATION AND PROPERTIES OF SECRETORY GRANULES FROM RAT ISLETS OF LANGERHANS

1969 • 153 citations

Galenics of Insulin: The Physico-chemical and Pharmaceutical Aspects of Insulin and Insulin Preparations

1987 • 148 citations

Landolt-Börnstein Zahlenwerte und Funktionen aus Physik, Chemie, Astronomie, Geophysik und Technik

1950 • 131 citations

Insulin forms amyloid in a strain‐dependent manner: An FT‐IR spectroscopic study

2004 • 126 citations

Stimulation of Insulin Fibrillation by Urea-induced Intermediates

2004 • 106 citations

An Indirect Chaotropic Mechanism for the Stabilization of Helix Conformation of Peptides in Aqueous Trifluoroethanol and Hexafluoro-2-propanol

1998 • 101 citations

Interaction of alcohols with proteins

1978 • 99 citations

Insulin Assembly Damps Conformational Fluctuations: Raman Analysis of Amide I Linewidths in Native States and Fibrils

2003 • 93 citations

Annular Oligomeric Amyloid Intermediates Observed by in Situ Atomic Force Microscopy

2004 • 88 citations

Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein–protein and protein–nucleic acid interactions

2002 • 87 citations

Acceleration of the folding of hen lysozyme by trifluoroethanol

1997 • 84 citations

Prediction of the association state of insulin using spectral parameters

2003 • 82 citations

Near-ultraviolet tyrosyl circular dichroism of pig insulin monomers, dimers, and hexamers. Dipole-dipole coupling calculations in the monopole approximation

1976 • 77 citations

High Pressure Promotes Circularly Shaped Insulin Amyloid

2004 • 76 citations

Equilibrium denaturation of insulin and proinsulin

1990 • 70 citations

The Cellular Prion Protein PrPc Is Expressed in Human Enterocytes in Cell-Cell Junctional Domains

2004 • 66 citations

Amyloid-like Fibril Formation in an All β-Barrel Protein

2003 • 62 citations

Effect of Aqueous Alcohol Solutions on the Thermal Transition of Lysozyme: A Calorimetric Study

1997 • 57 citations

Fluorescence Anisotropy: A Method for Early Detection of Alzheimer β-Peptide (Aβ) Aggregation

2001 • 55 citations

Hydration and structure—the two sides of the insulin aggregation process

2004 • 46 citations

Solvation properties and stability of ribonuclease A in normal and deuterated water studied by dielectric relaxation and differential scanning/pressure perturbation calorimetry

2004 • 46 citations

Pressure Perturbation Calorimetic Studies of the Solvation Properties and the Thermal Unfolding of Proteins in Solution

2003 • 26 citations

Prediction of density and excess volume for the ternary mixture: (water + 2,2,2-trifluoroethanol + 2,5,8,11,14-pentaoxapentadecane) from experimental binary values at temperatures from 283.15 K to 333.15 K

1995 • 22 citations

Crystallization and preliminary crystallographic investigation of a low-pH native insulin monomer with flexible behaviour

2001 • 17 citations

Deleted Work

1955 • 0 citations