Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Metal chelate affinity chromatography, a new approach to protein fractionation

1975 • 2,050 citations

Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region

1985 • 1,244 citations

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1987 • 1,107 citations

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1988 • 1,030 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1988 • 662 citations

The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer.

1990 • 584 citations

Cloning of a Transcriptionally Active Human TATA Binding Factor

1990 • 332 citations

Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.

1988 • 276 citations

One-step purification of hybrid proteins which have β-galactosidase activity

1984 • 265 citations

Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertions

1989 • 256 citations

Chelating peptide-immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins.

1988 • 237 citations

Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II

1989 • 193 citations

Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis.

1989 • 132 citations

Production in Escherichia coli and one‐step purification of bifunctional hybrid proteins which bind maltose

1988 • 127 citations

The signal for the termination of protein synthesis in procaryotes

1990 • 122 citations

Production of specific antibodies against protein A fusion proteins.

1986 • 77 citations

Molecular cloning and characterization of cDNA for eukaryotic transcription factor S-II.

1988 • 76 citations

Sea urchin USF: A helix-loop-helix protein active in embryonic ectoderm cells

1991 • 34 citations