Characterization of membrane proteins in detergent solutions

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

The Fluid Mosaic Model of the Structure of Cell Membranes

1972 • 8,835 citations

A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures

1961 • 5,561 citations

Physical chemistry of macromolecules

2007 • 3,839 citations

Computed circular dichroism spectra for the evaluation of protein conformation

1969 • 3,654 citations

Solubilization of membranes by detergents

1975 • 2,909 citations

Free Volume and Entropy in Condensed Systems III. Entropy in Binary Liquid Mixtures; Partial Molal Entropy in Dilute Solutions; Structure and Thermodynamics in Aqueous Electrolytes

1945 • 2,766 citations

Three-dimensional model of purple membrane obtained by electron microscopy

1975 • 2,259 citations

Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases

1966 • 2,239 citations

Proteins, amino acids and peptides

1943 • 2,189 citations

Physical chemistry of macromolecules

1962 • 2,105 citations

Rhodopsin-like Protein from the Purple Membrane of Halobacterium halobium

1971 • 2,075 citations

Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System

1971 • 2,019 citations

Ion-solvent interaction. Structural aspects of ion-solvent interaction in aqueous solutions: a suggested picture of water structure

1957 • 1,801 citations

Linked Functions and Reciprocal Effects in Hemoglobin: A Second Look

1964 • 1,613 citations

Phosphatidylcholine vesicles. Formation and physical characteristics

1969 • 1,522 citations

Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation

1971 • 1,364 citations

Classification of surface-active agents by "HLB"

1949 • 1,204 citations

The Molecular Organization of Membranes

1974 • 1,074 citations

Substructure of the myosin molecule

1971 • 1,062 citations

Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium

1975 • 994 citations

Purification and characterization of (Na+ + K+)-ATPase III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate

1974 • 936 citations

The Gross Conformation of Protein-Sodium Dodecyl Sulfate Complexes

1970 • 935 citations

Estimation of molecular radius, free mobility, and valence using polyacrylamide gel electrophoresis

1971 • 737 citations

Thermodynamic Analysis of Multicomponent Solutions

1964 • 717 citations

Reconstitution of Purple Membrane Vesicles Catalyzing Light-driven Proton Uptake and Adenosine Triphosphate Formation

1974 • 714 citations

Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents

1973 • 687 citations

Structure of crystalline α-chymotrypsin

1972 • 675 citations

Membrane Receptors

1974 • 636 citations

Selective solubilization of proteins from red blood cell membranes by protein perturbants

1973 • 611 citations

The Estimation of Polypeptide Chain Molecular Weights by Gel Filtration in 6 M Guanidine Hydrochloride

1969 • 603 citations

Theory of micelle formation in aqueous solutions

1974 • 553 citations

Human erythrocyte membrane glycoprotein: A re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis

1971 • 552 citations

Molecular characterization of proteins in detergent solutions

1974 • 492 citations

Multiple Equilibria in Proteins

1969 • 475 citations

Isolation of sarcoplasmic reticulum by zonal centrifugation and purification of Ca2+-pump and Ca2+-binding proteins

1973 • 474 citations

The size and detergent binding of membrane proteins.

1975 • 449 citations

Tables for estimating sedimentation through linear concentration gradients of sucrose solution

1967 • 445 citations

Membrane-bound enzymes and membrane ultrastructure

1973 • 444 citations

Cholesterol is excluded from the phospholipid annulus surrounding an active calcium transport protein

1975 • 391 citations

The Simultaneous Determination of Partial Specific Volumes and Molecular Weights with Microgram Quantities

1967 • 388 citations

INTRAMEMBRANE PARTICLE AGGREGATION IN ERYTHROCYTE GHOSTS

1974 • 348 citations

A Form of Reduced Nicotinamide Adenine Dinucleotide-Cytochrome b5 Reductase Containing Both the Catalytic Site and an Additional Hydrophobic Membrane-binding Segment

1973 • 344 citations

Cytochrome c Oxidase from Bakers' Yeast

1973 • 331 citations

Membrane Proteins in Transport and Phosphorylation

1975 • 321 citations

Morphology of the isolated hemagglutinin and neuraminidase subunits of influenza virus

1969 • 321 citations

Purification of the Sodium- and Potassium-dependent Adenosine Triphosphatase from Canine Renal Medulla

1971 • 319 citations

ANIONIC SITES OF HUMAN ERYTHROCYTE MEMBRANES

1973 • 307 citations

Studies on the Characterization of the Sodium-Potassium Transport Adenosine Triphosphatase

1973 • 305 citations

Reversible lipid titrations of the activity of pure adenosine triphosphatase-lipid complexes

1974 • 296 citations

The Binding of Divers Detergent Anions to Bovine Serum Albumin^*

1967 • 291 citations

Sugar Transport

1971 • 290 citations

Binding of deoxycholate, Triton X-100, sodium dodecyl sulfate, and phosphatidylcholine vesicles to cytochrome b₅

1975 • 287 citations

The Binding of Cytochrome b5 to Liver Microsomes

1972 • 286 citations

ATPase of Escherichia coli: purification, dissociation, and reconstitution of the active complex from the isolated subunits

1976 • 270 citations

Properties of the Two Polypeptides of Sodium- and Potassium-dependent Adenosine Triphosphatase

1972 • 267 citations

Structure of visual pigments. I. Purification, molecular weight, and composition of bovine visual pigment500

1968 • 266 citations

Measurement of Free Electrophoretic Mobility and Retardation Coefficient of Protein-Sodium Dodecyl Sulfate Complexes by Gel Electrophoresis

1972 • 256 citations

Lipid requirements for rhodopsin regenerability

1973 • 250 citations

Freeze-etch localization of concanavalin A receptors to the membrane intercalated particles of human erythrocyte ghost membranes

1974 • 246 citations

Purification and Properties of the Cholinergic Receptor Protein from Electrophorus electricus Electric Tissue

1974 • 238 citations

Subunit composition, function, and spatial arrangement in the Ca2+- and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium

1975 • 228 citations

Hydrophobic free energy, micelle formation and the association of proteins with amphiphiles

1972 • 219 citations

Mitochondrial adenosine triphosphatase

1975 • 218 citations

Three-Dimensional Structure of Immunoglobulins

1975 • 218 citations

The Interaction of a Cationic Detergent with Bovine Serum Albumin and Other Proteins

1974 • 216 citations

THE MOLECULAR WEIGHT OF RHODOPSIN AND THE NATURE OF THE RHODOPSIN-DIGITONIN COMPLEX

1954 • 214 citations

Rhodopsin. Purification and recombination with phospholipids assayed by the metarhodopsin I .far. metarhodopsin II transition

1974 • 214 citations

Gross Conformation of Rabbit 7 S γ-Immunoglobulin and Its Papain-cleaved Fragments

1965 • 212 citations

Similarity of the Active Site of Phosphorylation of the Adenosine Triphosphatase for Transport of Sodium and Potassium Ions in Kidney to That for Transport of Calcium Ions in the Sarcoplasmic Reticulum of Muscle

1973 • 206 citations

Isolation and characterization of the components of the sodium pump

1974 • 205 citations

Cytochrome c oxidase from bakers' yeast. III. Physical characterization of isolated subunits and chemical evidence for two different classes of polypeptides

1975 • 198 citations

Lipid Requirement of Membrane‐Bound ATPase

1973 • 192 citations

Solubilization of the membrane proteins from Semliki Forest virus with Triton X100

1973 • 189 citations

Studies on Glycopeptides Released by Trysin from Intact Human Erythrocytes^*

1967 • 185 citations

Purification by Means of Detergents and Properties of Cytochrome b5 from Liver Microsomes

1968 • 185 citations

Use of gel chromatography for the determination of the Stokes radii of proteins in the presence and absence of detergents. A reexamination

1976 • 180 citations

Studies on Phosphatidylcholine Vesicles

1971 • 180 citations

Retention of enzyme activity by detergent-solubilized sarcoplasmic Ca²⁺ion-activated ATPase

1976 • 178 citations

Purification of acetylcholine receptors from Torpedo claifornica electroplax by affinity chromatography

1973 • 176 citations

Studies on the cholinergic receptor protein from Electrophorus electricus Effect of detergents on some hydrodynamic properties of the receptor protein in solution

1972 • 172 citations

Studies on the Location and Orientation of Proteins in the Sarcoplasmic Reticulum

1973 • 171 citations

Intensity fluctuation spectroscopy of laser light scattered by solutions of spherical viruses. R17, Q.beta., BSV, PM2, and T7. I. Light-scattering technique

1974 • 170 citations

Arrangement of the subunits in solubilized and membrane-bound cytochrome c oxidase from bovine heart.

1975 • 158 citations

Phosphorylation of endogenous substrates by erythrocyte membrane protein kinases. I. Monovalent cation-stimulated reaction

1974 • 155 citations

Characterization of the Membrane Protein Component of the Lactose Transport System of Escherichia coli

1969 • 154 citations

Reconstitution of cytochrome oxidase vesicles and conferral of sensitivity to energy transfer inhibitors

1972 • 144 citations

Reconstitution of Active Transport Catalyzed by the Purified Sodium and Potassium Ion-stimulated Adenosine Triphosphatase from Canine Renal Medulla

1974 • 143 citations

A cholate‐dilution procedure for the reconstitution of the Ca⁺⁺ pump, ³²P_i—ATP exchange, and oxidative phosphorylation

1975 • 141 citations

Assembly of the Mitochondrial Membrane System

1973 • 137 citations

Reinvestigation of a region of the fd bacteriophage coat protein sequence

1974 • 136 citations

Isolation and characterization of tryptic fragments of the adenosine triphosphatase of sarcoplasmic reticulum.

1976 • 136 citations

Stepwise dissociation of the semliki forest virus membrane with triton-X-100

1973 • 132 citations

Rhodopsin is the Predominant Protein of Rod Outer Segment Membranes

1972 • 131 citations

Light dissociates enzymatically-cleaved rhodopsin into two different fragments

1975 • 130 citations

Sodium Transport by Phospholipid Vesicles Containing Purified Sodium and Potassium Ion-activated Adenosine Triphosphatase

1974 • 127 citations

The interaction of tubulin and other proteins with structure-stabilizing solvents

1976 • 124 citations

Tryptic cleavage of sarcoplasmic reticulum protein

1974 • 117 citations

Limitations of the detergent-polyacrylamide gel electrophoresis niethod for molecular weight determination of proteins

1971 • 117 citations

Physical state of cytochrome oxidase: Relationship between membrane formation and ionic strength

1968 • 113 citations

A coupling factor from sarcoplasmic reticulum required for the translocation of Ca2+ ions in a reconstituted Ca2+ATPase pump.

1975 • 110 citations

Interaction of deoxycholate and of detergents with the coat protein of bacteriophage f1

1975 • 107 citations

Interaction of L-alpha-palmitoyl lysophosphatidylcholine with the AI polypeptide of high density lipoprotein.

1975 • 100 citations

Molecular weights and hydrophobicity of the polypeptide chain of sarcoplasmic reticulum calcium(II) adenosine triphosphatase and of its primary tryptic fragments

1976 • 100 citations

Biological membrane structure. III. The lattice structure of membranous cytochrome oxidase

1972 • 99 citations

Evidence for a major conformational change of coat protein in assembly of fl bacteriophage

1976 • 99 citations

Molecular aggregation in aqueous dispersions of phosphatidyl and lysophosphatidyl cholines

1966 • 96 citations

Bovine Rhodopsin: Characterization of the Complex Formed with Triton X-100

1974 • 92 citations

Physical chemical studies of short-chain lecithin homologues. II

1974 • 92 citations

Cytochrome c oxidase from bakers' yeast. IV. Immunological evidence for the participation of a mitochondrially synthesized subunit in enzymatic activity

1975 • 92 citations

Lipid specificity of beta-hydroxybutyrate dehydrogenase activation.

1975 • 91 citations

The interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes.

1975 • 88 citations

Studies on the characterization of the sodium—Potassium transport adenosine triphosphatase

1974 • 86 citations

Biochemical aspects of the visual process XX. The molecular weight of rhodopsin

1972 • 82 citations

A coat protein from bacteriophage fd

1966 • 82 citations

Two-domain structure of cytochrome b₅ in deoxycholate solution

1975 • 80 citations

Solubility of Membrane Proteins in Aqueous Media

1971 • 76 citations

Isolation of a Bacterial Membrane Protein, Nectin, Essential for the Attachment of Adenosine Triphosphatase

1971 • 72 citations

The protein composition of sarcoplasmic reticulum membranes

1969 • 70 citations

Acetylcholinesterase of the human erythrocyte membrane

1970 • 69 citations

Studies on the electron-transfer system LX. Molecular weights of some components of the electron transfer chain in beef-heart mitochondria

1965 • 68 citations

Separation of phospholipids from glucose-6-phosphatase by gel chromatography. Specificity of phospholipid reactivation

1972 • 66 citations

MEMBRANE PROTEINS: A PERSPECTIVE

1972 • 63 citations

Lipid–protein and lipid–lipid interactions in cytochrome oxidase model membranes

1973 • 63 citations

Molecular Weight of Two States of Cytochrome c Oxidase

1970 • 63 citations

Separation and Characterisation of Tryptic Fragments from the Adenosine Triphosphatase of Sarcoplasmic Reticulum

1975 • 63 citations

The major human erythrocyte membrane protein. Evidence for an S-shaped structure which traverses the membrane twice and contains a duplicated set of sites.

1975 • 62 citations

The Interaction of Polypeptide Components of Human High Density Lipoprotein with Sodium Dodecyl Sulfate

1974 • 61 citations

Erythrocyte spectrin. Purification in deoxycholate and preliminary characterization

1976 • 60 citations

The molecular weight of an acetylcholine receptor isolated from Torpedo californica

1975 • 60 citations

Some molecular properties of an isolated acetylcholine receptor ion-translocation protein

1974 • 57 citations

Subunit structure of the cytochrome c oxidase complex

1975 • 57 citations

Two-dimensional separation of erythrocyte membrane proteins

1975 • 57 citations

Solubilization of the Semliki Forest virus membrane with sodium dodecyl sulfate

1975 • 55 citations

Molecular weight of the acetylcholine receptors of electric organs and the effect of Triton X-100.

1975 • 55 citations

Purification and Some Properties of Na, K-transport ATPase

1973 • 54 citations

Molecular sieve studies of interacting protein systems

1971 • 54 citations

Extraction and properties of aldehyde dehydrogenase from Pseudomonas aeruginosa

1963 • 54 citations

Electric and nonelectric interactions of a nonionic-cationic micelle

1974 • 48 citations

Light scattering and diffusion studies in nonionic micellar systems

1972 • 45 citations

Restoration of coupling factor activity to Escherichia coli ATPase missing the delta subunit

1975 • 42 citations

The Interaction of Polypeptide Components of Human High Density Serum Lipoprotein with Detergents

1974 • 42 citations

Micellar aggregation properties of some Zwitterionic N-alkyl betaines

1970 • 38 citations

f1 Coat protein synthesis and altered phospholipid metabolism in f1 infected Escherichia coli

1974 • 37 citations

Ca2+ uptake in reconstituted sarcoplasmic reticulum vesicles

1973 • 36 citations

Effect of cytochrome b5 on the size, density, and permeability of phosphatidylcholine vesicles

1975 • 31 citations

Structure and synthesis of a lipid-containing bacteriophage XIX. Reconstitution of bacteriophage PM2 In Vitro

1975 • 30 citations

Extraction, regeneration after bleaching, and ion-exchange chromatography of rhodopsin in Tween 80

1973 • 28 citations

Hydrophobic interactions of the apo-Gln-I polypeptide component of human high density serum lipoprotein.

1975 • 28 citations

Absorption Spectra and Enzymatic Properties of Monomer and Dimer States of Cytochrome c Oxidase

1970 • 26 citations

A simplified procedure for the preparation of cytochrome oxidase by non-ionic detergent

1967 • 16 citations