Cloning and expression of cDNA of human Δ⁴‐3‐oxosteroid 5β‐reductase and substrate specificity of the expressed enzyme

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES

1957 • 63,238 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

1986 • 4,200 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

λ ZAP: a bacteriophage λ expression vector within vivoexcision properties

1988 • 1,394 citations

Bile acid biosynthesis

1992 • 790 citations

The Aldo-Keto Reductase Superfamily

1989 • 455 citations

An Unlikely Sugar Substrate Site in the 1.65 Å Structure of the Human Aldose Reductase Holoenzyme Implicated in Diabetic Complications

1992 • 431 citations

Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.

1991 • 353 citations

Delta 4-3-oxosteroid 5 beta-reductase deficiency described in identical twins with neonatal hepatitis. A new inborn error in bile acid synthesis.

1988 • 246 citations

Cloning and sequencing of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase

1991 • 148 citations

Chapter 9 Mechanism of bile acid biosynthesis in mammalian liver

1985 • 128 citations

Degradation of apolipoprotein II mRNA occurs via endonucleolytic cleavage at 5′-AAU-3′/5′-UAA-3′ elements in single-stranded loop domains of the 3′-noncoding region

1989 • 118 citations

Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7α‐hydroxylase

1990 • 114 citations

Conversion of Cholesterol to Bile Acids in Rat: Purification and Properties of a Δ⁴‐3‐Ketosteroid 5β‐Reductase and a 3α‐Hydroxysteroid Dehydrogenase

1967 • 75 citations

Synthesis and Metabolism of Cholest-4-ene-7α, 12α-diol-3-one and 5β-Cholestane-7α,12α-diol-3-one

1965 • 67 citations

Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase.

1984 • 67 citations

Enzymatic Conversion of a Δ⁴‐3‐Ketosteroid into a 3α‐Hydroxy‐5β‐Steroid: Mechanism and Stereochemistry of Hydrogen Transfer from NADPH

1967 • 59 citations

Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins.

1990 • 52 citations

Molecular cloning and sequence analysis of cDNA encoding δ⁴-3-ketosteroid 5β-reductase of rat liver

1991 • 47 citations

Stereochemistry of Hydrogen Transfer from Pyridine Nucleotides Catalyzed by Δ⁴‐3‐Oxosteroid 5β‐Reductase and 3α‐Hydroxysteroid Dehydrogenase from Rat Liver

1970 • 41 citations

Stereochemistry of the Enzymatic Conversion of a Δ⁴‐3‐Oxosteroid into a 3‐Oxo‐5β‐Steroid

1969 • 36 citations

Identification of lysine 134 in the steroid-binding site of the sex steroid-binding protein of human plasma.

1990 • 34 citations

The sexually differentiated delta 4-3-ketosteroid 5 beta-reductase of rat liver. Purification, characterization, and quantitation.

1985 • 17 citations

Δ⁴-3-Oxosteroid 5β-Reductase. Structure and Function

1991 • 15 citations

Identification of cortisone 5β-reductase as Δ4-3-ketosteroid 5β-reductase

1987 • 8 citations

Identification of 7α,12α-dihydroxy-5β-cholestan-3-one 3α-reductase as 3α-hydroxysteroid dehydrogenase

1986 • 8 citations