Transformation by polyoma virus is drastically reduced by substitution of phenylalanine for tyrosine at residue 315 of middle-sized tumor antigen.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Rapid and efficient cosmid cloning

1981 • 1,897 citations

Preparative and analytical purification of DNA from agarose.

1979 • 1,585 citations

Agar suspension culture for the selective assay of cells transformed by polyoma virus

1964 • 1,265 citations

Protein kinase activity associated with the avian sarcoma virus src gene product.

1978 • 1,137 citations

Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein

1978 • 673 citations

An activity phosphorylating tyrosine in polyoma T antigen immunoprecipitates

1979 • 540 citations

Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine

1980 • 539 citations

Polyoma virus transforming protein associates with the product of the c-src cellular gene

1983 • 497 citations

Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein

1981 • 435 citations

The roles of individual polyoma virus early proteins in oncogenic transformation

1982 • 366 citations

[75] Assay of transforming activity of tumor virus DNA

1980 • 331 citations

Purification of polyoma virus

1963 • 298 citations

Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus

1980 • 226 citations

Local mutagenesis of Rous sarcoma virus: The major sites of tyrosine and serine phosphorylation of p60src are dispensable for transformation

1983 • 189 citations

Phosphorylation of polyoma T antigens

1979 • 186 citations

Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.

1980 • 176 citations

Tumor antigen(s) in cells productively infected by wild-type polyoma virus and mutant NG-18

1978 • 176 citations

Protein kinase activity associated with polyoma virus middle T antigen in vitro

1979 • 174 citations

Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation.

1982 • 142 citations

Tumor antigens induced by nontransforming mutants of polyoma virus

1978 • 141 citations

Phosphorylation of tyrosine-416 is not required for the transforming properties and kinase activity of pp60v-src

1983 • 138 citations

Comparison of phosphorylation of two polyoma virus middle T antigens in vivo and in vitro

1981 • 138 citations

Rous sarcoma virus transforming protein, p60src, expressed in E. coli, functions as a protein kinase

1981 • 126 citations

New classes of viable deletion mutants in the early region of polyoma virus

1979 • 116 citations

Comparisons of two early gene functions essential for transformation in polyoma virus and SV-40

1979 • 115 citations

Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues.

1982 • 99 citations

Transforming activity of polyoma virus middle-T antigen probed by site-directed mutagenesis

1983 • 82 citations

Construction and analysis of viable deletion mutants of polyoma virus

1979 • 80 citations

Analysis of host range of nontransforming polyoma virus mutants

1975 • 77 citations

Polyoma virus middle T antigen: relationship to cell membranes and apparent lack of ATP-binding activity.

1982 • 73 citations

Identification of DNA sequence changes leading to loss of transforming ability in polyoma virus.

1980 • 68 citations

Differential subcellular localization of in vivo-phosphorylated and nonphosphorylated middle-sized tumor antigen of polyoma virus and its relationship to middle-sized tumor antigen phosphorylating activity in vitro.

1982 • 56 citations

Polyoma Virus Middle T Antigen: Relationship to Cell Membranes and Apparent Lack of ATP-Binding Activity

1982 • 46 citations

Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity.

1982 • 37 citations

Deletion mapping of a short polyoma virus middle T antigen segment important for transformation

1983 • 36 citations

Requirement for the C-terminal region of middle T-antigen in cellular transformation by virus

1981 • 36 citations