Characterization of phosphate binding in the active site of barnase by site-directed mutagenesis and NMR

Fundamentals of Enzyme Kinetics

1979 • 2,137 citations

Electrostatic Interactions in Macromolecules: Theory and Applications

1990 • 1,324 citations

The α-helix dipole and the properties of proteins

1978 • 985 citations

Three-Dimensional Structure of an Oncogene Protein: Catalytic Domain of Human c-H- ras P21

1988 • 532 citations

Energetics of complementary side chain packing in a protein hydrophobic core

1989 • 346 citations

Molecular structure of a new family of ribonucleases

1982 • 222 citations

Prediction of electrostatic effects of engineering of protein charges

1987 • 220 citations

Crystallographic analysis of the complex between triosephosphate isomerase and 2-phosphoglycolate at 2.5-.ANG. resolution: implications for catalysis

1990 • 216 citations

Relationships between apparent binding energies measured in site-directed mutagenesis experiments and energetics of binding and catalysis

1988 • 172 citations

Dipoles localized at helix termini of proteins stabilize charges.

1991 • 170 citations

Binding of phosphate ligands to ribonuclease A

1968 • 159 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

The structural and sequence homology of a family of microbial ribonucleases

1983 • 141 citations

Kinetic characterization of the recombinant ribonuclease from Bacillus amyloliquefaciens (barnase) and investigation of key residues in catalysis by site-directed mutagenesis

1989 • 130 citations

Fluorescence spectrum of barnase: contributions of three tryptophan residues and a histidine-related pH dependence

1991 • 129 citations

Determination of the three-dimensional solution structure of barnase using nuclear magnetic resonance spectroscopy

1991 • 122 citations

Anion binding and pH-dependent electrostatic effects in ribonuclease

1982 • 117 citations

Ribonuclease T1 is stabilized by cation and anion binding

1988 • 115 citations

Histidine-40 of ribonuclease T1 acts as base catalyst when the true catalytic base, glutamic acid-58, is replaced by alanine

1990 • 111 citations

Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A

1987 • 101 citations

Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation

1987 • 67 citations

NMR study of the phosphoryl binding loop in purine nucleotide proteins: evidence for strong hydrogen bonding in human N-ras p21

1990 • 49 citations

Salt-dependent structure change and ion binding in cytochrome c studied by two-dimensional proton NMR

1990 • 47 citations

Sequential assignment of the proton nuclear magnetic resonance spectrum of barnase

1990 • 37 citations

Metabolism and Enzymology of Nucleic Acids: Including Gene Manipulations

2011 • 37 citations

Sequence-specific proton NMR assignments and secondary structure of eglin c

1990 • 23 citations

Structural and dynamic differences between normal and transforming N-ras gene products: a phosphorus-31 and isotope-edited proton NMR study

1989 • 20 citations

Inhibitors and activators

1979 • 16 citations

Structure Bases for Nucleotide Recognition by Guanyl-Specific Ribonucleases

1988 • 4 citations