Biomedical and biotechnological applications of PEG- and PM-modified proteins

Detection and molecular weight determination of polyethylene glycol-modified hirudin by staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

1992 • 208 citations

Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins.“

1992 • 121 citations

Analysis of micelle formation of an adriamycin-conjugated poly(ethylene glycol)-poly(aspartic acid) block copolymer by gel permeation chromatography.

1993 • 99 citations

Prolonged immunostimulatory effect of low-dose polyethylene glycol interleukin 2 in patients with human immunodeficiency virus type 1 infection.

1993 • 82 citations

MODIFICATION OF E. COLI ASPARAGINASE WITH 2,4-BIS(O-METHOXYPOLYETHYLENE GLYCOL)-6-CHLORO-S-TRIAZINE(ACTIVATED PEG₂); DISAPPEARANCE OF BINDING ABILITY TOWARDS ANTI-SERUM AND RETENTION OF ENZYMIC ACTIVITY

1980 • 78 citations

Cellulase immobilization on Fe₃O₄ and characterization

1989 • 68 citations

Preparation and enzymic activity of poly[(N-acylimino)ethylene]-modified catalase

1990 • 60 citations

On-line characterization of polyethylene glycol-modified proteins

1991 • 56 citations

Ester synthesis from ?-substituted carboxylic acid catalyzed by polyethylene glycol-modified lipase fromCandida cylindracea in benzene

1986 • 48 citations

Electron-transfer reaction of polyethylene oxide-modified myoglobin in polyethylene oxide oligomers

1992 • 46 citations

Biochemical and biopharmaceutical properties of macromolecular conjugates of uricase with dextran and polyethylene glycol.

1990 • 43 citations

Polyethylene glycol-conjugated superoxide dismutase protects rats against oxygen toxicity

1993 • 42 citations

Uptake, intracellular transport, and degradation of polyethylene glycol-modified asialofetuin in hepatocytes

1992 • 41 citations

Effects of Coupling Chemistry on the Activity of Poly(ethylene glycol)-Modified Alkaline Phosphatase

1989 • 40 citations

Subtilisin-catalysed peptide synthesis and transesterification in organic solvents

1990 • 39 citations

Glycosidases in organic solvents: II. Transgalactosylation catalysed by polyethylene glycol-modified β-galactosidase

1990 • 39 citations

Active Site Protection of Proteolytic Enzymes by Poly(ethylene glycol) Surface Modification

1993 • 39 citations

Biological activities of polyethylene-glycol immunoglobulin conjugates resistance to enzymatic degradation

1992 • 38 citations

Polyethylene glycol (PEG) modification of granulocyte‐macrophage colony stimulating factor (GM‐CSF) enhances neutrophil priming activity but not colony stimulating activity

1992 • 35 citations

Selective synthesis of 2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine as a protein modifier

1991 • 35 citations

Polyethylene glycol-modified hemin having peroxidase activity in organic solvents

1986 • 33 citations

Glucose-sensing carbon paste electrode containing polyethylene glycol-modified glucose oxidase

1992 • 31 citations

Stabilization of L-Asparaginase Modified with Comb-Shaped Poly(ethylene glycol) Derivatives, in vivo and in vitro

1994 • 31 citations

Applications of polymer electrolytes: Electrochromics, sensors and biology

1992 • 29 citations

Photo-stable chlorophylls conjugated with montmorillonite

1992 • 28 citations

Lipase made active in hydrophobic media by coupling with polyethylene glycol

1994 • 27 citations

Solid phase synthesis of peptides with polyethylene glycol-modified protease in organic solvents

1990 • 24 citations

Chemical modification of lipase with a comb-shaped synthetic copolymer of polyoxyethylene allyl methyl diether and maleic anhydride

1992 • 23 citations

Catalytic Activity of Administered Gulonolactone Oxidase Polyethylene Glycol Conjugates

1989 • 22 citations

Reactions Catalyzed by Peg-Modified α-Chymotrypsin in Organic Solvents. Influence of Water Content and Degree of Modification

1993 • 21 citations

Catabolism of streptokinase and polyethylene glycol-streptokinase: evidence for transport of intact forms through the biliary system in the mouse

1990 • 21 citations

Chemical modification of Vibrio vulnificus metalloprotease with activated polyethylene glycol

1993 • 18 citations

Analysis of T-cell receptor alpha beta chains of CD8+ suppressor T cells induced by tolerogenic conjugates of antigen and monomethoxypolyethylene glycol. Involvement of TCR alpha-CDR3 domain in immunosuppression.

1997 • 15 citations

Characterization of a polyethylene glycol conjugate of recombinant human interferon-gamma.

1990 • 15 citations

Mitigation of Pulmonary Oxygen Toxicity in Rats by Intratracheal Instillation of Polyethylene Glycol-Conjugated Antioxidant Enzymes

1993 • 15 citations

Epoxy-oxirane activation of PEG for protein ligand coupling

1989 • 14 citations

Southern blot analysis with synthetic oligonucleotides. Application to prostatic protein genes

1990 • 14 citations

Reduction of Immunoreactivity of Bovine Serum Albumin Conjugated with Comb-Shaped Polyethylene Glycol Derivatives

1993 • 13 citations

Preparation of (Mrg-Gly-Asp)-(amino-poly(ethylene glycol)) hybrids and their inhibitory effect on experimental metastasis.

1991 • 13 citations

Alcoholysis of ϵ-Decalactone with Polyethylene Glycol-Modified Lipase in 1,1,1-Trichloroethane

1994 • 12 citations

Lactone synthesis from 16-hydroxyhexadecanoic acid ethyl ester in organic solvents catalyzed with polyethylene glycol-modified lipase

1993 • 12 citations

Targeting of urokinase by magnetic force.

1989 • 11 citations

Analysis of T-cell receptor alpha beta chains of CD8+ suppressor T cells induced by tolerogenic conjugates of antigen and monomethoxypolyethylene glycol. Involvement of TCR alpha-CDR3 domain in immunosuppression.

1993 • 11 citations

Protein–protein interaction in low water organic media

1990 • 10 citations

Modification of two amino groups with polyethylene glycol causes a unique activity change of an alkaline proteinase from alkalophilicBacillus sp.

1989 • 10 citations

POLYETHYLENE GLYCOL‐MODIFIED HEMATOPORPHYRIN AS PHOTOSENSITIZER

1988 • 9 citations

Synthesis of gefarnate with reusable lipase modified with polyethylene glycol

1989 • 9 citations

Changes in Enzymatic and Membrane-Adsorbing Activities of Sphingomyelinase from Bacillus cereus by Modification with a Polyethylene Glycol Derivative.

1992 • 8 citations

Synthesis of N?,N?-dicarbobenzoxy-L-ornithyl-?-alanine benzyl ester, a derivative of salty peptide, in 1,1,1-trichloroethane with polyethylene glycol-modified papain

1989 • 5 citations

Kinetic Basis for Artificially Designing Enzymes

1990 • 2 citations

PEG-papain catalyzed synthesis of a Kyotorphin derivative in aqueous organic media

1993 • 2 citations

Synthesis of Enkephalins Using Modified Proteases in Organic Media

1992 • 2 citations

Deleted Work

1955 • 0 citations

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