A vector for controlled, high-yield production of specifically mutated proteins in Escherichia coll: test of a putative cytidine-binding domain in Rho factor and its Thr16→ Ala mutant

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering

1986 • 816 citations

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

1991 • 794 citations

Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.

1985 • 184 citations

Use of a chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA.

1988 • 184 citations

Structural asymmetry in the CTP-liganded form of aspartate carbamoyltransferase from Escherichia coli

1987 • 135 citations

Sequence elements essential for rho-dependent transcription termination at lambda tR1.

1987 • 87 citations

Identification and characterization of transcription termination sites in the Escherichia coli lacZ gene

1989 • 72 citations

Transcription termination factor rho has three distinct structural domains.

1990 • 66 citations

The use of native T7 DNA polymerase for site-directed mutagenesis

1989 • 61 citations

Thermodynamic and enzymological characterization of the interaction between transcription termination factor .rho. and .lambda. cro mRNA

1989 • 40 citations

Enhancement of transcription termination factor rho activity with potassium glutamate

1991 • 32 citations